FastQCFastQC Report
Sat 18 Jun 2016
SRR3551958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364067
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36841.0119016554645162No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG35680.9800393883543413No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC33090.9088986367893822No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC31210.8572597900935817Illumina PCR Primer Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC14090.38701667550203667Illumina PCR Primer Index 11 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT8360.229628062966432No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC7540.20710473621613604No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC7160.19666709699038915Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT6390.17551714382242828Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT5700.15656458838620363No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC5680.1560152389532696No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT5180.14228150312991839Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA4620.12689971900776503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA207.0269307E-445.016
TTGTGCG502.1827873E-1145.01
CACGGCC207.0269307E-445.017
AACTTCG253.885537E-545.011
CGATTCG207.0269307E-445.010
CGTTCAT207.0269307E-445.017
CCCGTTA406.7921064E-945.040
AGGTTCG207.0269307E-445.043
GGAACGA207.0269307E-445.08
GCGATTC253.885537E-545.09
CGTAAGG351.208864E-745.02
ACCCGTT351.208864E-745.039
CGCAATT253.885537E-545.020
CGGTAGT253.885537E-545.012
CGTCATA207.0269307E-445.038
CCGTTAC207.0269307E-445.041
CCGTTAA207.0269307E-445.041
CCGCAAT207.0269307E-445.019
AGGTCGG207.0269307E-445.02
CGGACCT207.0269307E-445.021