##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364067
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27572397388393	33.0	31.0	34.0	30.0	34.0
2	32.362790365509646	34.0	31.0	34.0	30.0	34.0
3	32.36820145742404	34.0	31.0	34.0	30.0	34.0
4	35.93887388859743	37.0	35.0	37.0	35.0	37.0
5	35.99261949036853	37.0	35.0	37.0	35.0	37.0
6	35.97930051336704	37.0	35.0	37.0	35.0	37.0
7	36.168639288922094	37.0	36.0	37.0	35.0	37.0
8	35.96607767251632	37.0	35.0	37.0	35.0	37.0
9	37.89854339997858	39.0	38.0	39.0	35.0	39.0
10	37.59048471847215	39.0	37.0	39.0	35.0	39.0
11	37.54540510400558	39.0	37.0	39.0	35.0	39.0
12	37.52188470803451	39.0	37.0	39.0	35.0	39.0
13	37.50708798105843	39.0	37.0	39.0	35.0	39.0
14	38.7809661408477	40.0	38.0	41.0	35.0	41.0
15	38.77075922838379	40.0	38.0	41.0	35.0	41.0
16	38.830108194370816	40.0	38.0	41.0	35.0	41.0
17	38.74911486072619	40.0	38.0	41.0	35.0	41.0
18	38.648839361985566	40.0	38.0	41.0	35.0	41.0
19	38.55804563445739	40.0	37.0	41.0	35.0	41.0
20	38.40266489409916	40.0	37.0	41.0	34.0	41.0
21	38.37082460096631	40.0	37.0	41.0	34.0	41.0
22	38.385753171806286	40.0	37.0	41.0	34.0	41.0
23	38.32351737454919	40.0	37.0	41.0	34.0	41.0
24	38.29364375238624	40.0	37.0	41.0	34.0	41.0
25	38.18028000340597	40.0	37.0	41.0	34.0	41.0
26	38.21511150420115	40.0	37.0	41.0	34.0	41.0
27	38.172168859028694	40.0	37.0	41.0	34.0	41.0
28	38.10778235874166	40.0	37.0	41.0	34.0	41.0
29	38.0532868949946	40.0	37.0	41.0	34.0	41.0
30	37.87914587150168	40.0	36.0	41.0	34.0	41.0
31	37.82803440026149	40.0	36.0	41.0	33.0	41.0
32	37.65551121084855	40.0	36.0	41.0	33.0	41.0
33	37.51450419840304	40.0	36.0	41.0	33.0	41.0
34	37.37092073711707	40.0	36.0	41.0	33.0	41.0
35	37.24675677828532	40.0	36.0	41.0	33.0	41.0
36	37.17554186454691	40.0	36.0	41.0	32.0	41.0
37	37.09840221717431	40.0	36.0	41.0	32.0	41.0
38	37.057862975770945	40.0	36.0	41.0	32.0	41.0
39	37.00383720578905	40.0	35.0	41.0	32.0	41.0
40	36.942266121345796	40.0	35.0	41.0	31.0	41.0
41	36.8277459918092	40.0	35.0	41.0	31.0	41.0
42	36.73603759747519	40.0	35.0	41.0	31.0	41.0
43	36.59476140380754	39.0	35.0	41.0	31.0	41.0
44	36.65545902265242	39.0	35.0	41.0	31.0	41.0
45	36.63000216993026	39.0	35.0	41.0	31.0	41.0
46	36.53676383742553	39.0	35.0	41.0	31.0	41.0
47	36.41018274108887	39.0	35.0	41.0	31.0	41.0
48	36.41442921220545	39.0	35.0	41.0	31.0	41.0
49	36.40381303441399	39.0	35.0	41.0	31.0	41.0
50	36.27564157146899	39.0	35.0	41.0	31.0	41.0
51	35.44256963690749	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	6.0
11	3.0
12	4.0
13	3.0
14	11.0
15	12.0
16	22.0
17	51.0
18	85.0
19	142.0
20	341.0
21	641.0
22	1006.0
23	1293.0
24	1786.0
25	2450.0
26	3316.0
27	4016.0
28	3974.0
29	4064.0
30	4329.0
31	5293.0
32	7010.0
33	9459.0
34	16673.0
35	24625.0
36	23839.0
37	35206.0
38	69011.0
39	145237.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3086052841922	20.03175239722359	24.355956458563945	14.303685860020272
2	31.89605210030022	28.26897246935317	23.6041717595937	16.23080367075291
3	29.081185605946157	26.810999074346203	28.00775681399303	16.100058505714607
4	26.84396004032225	24.219443124479834	31.54336976435656	17.393227070841355
5	27.489170949303286	27.800652077776896	25.498603279066767	19.211573693853055
6	23.99448453169334	35.88432898340141	25.94989383822209	14.171292646683165
7	75.47237184364415	5.36879200806445	14.791233481749238	4.3676026665421475
8	75.28943848247712	8.555842743231329	11.278143858136003	4.876574916155542
9	69.7822104173133	7.425556284969524	12.944870037657905	9.847363260059275
10	34.256331938901354	32.153971659062755	19.501904869158697	14.087791532877189
11	27.566079869914056	23.18062334680155	30.53833497680372	18.714961806480677
12	24.270532621742703	20.732996948363898	33.26283348944013	21.73363694045327
13	24.644639585570786	22.25222280514301	35.39842940997124	17.70470819931496
14	21.058211812660858	27.55701560427064	30.648754212823466	20.736018370245038
15	18.672387225428285	24.361999302326222	36.4465881280094	20.519025344236088
16	21.02552552140128	24.37683173701544	31.531284076832012	23.06635866475127
17	20.19683190182027	24.759453617054003	31.957853911505303	23.08586056962043
18	20.37784253997204	23.857696522892763	32.776109891860564	22.988351045274634
19	21.543562036658088	25.53815643823802	30.386714533313924	22.531566991789973
20	22.858979253818664	25.67109900100806	32.08420428108013	19.385717464093148
21	22.323088881991502	27.265311055382664	31.192610151428173	19.218989911197664
22	20.442665773058255	23.82858100294726	30.97781452315096	24.750938700843523
23	19.847720337190683	27.180436567994352	30.62705491022257	22.344788184592396
24	20.875553126210285	25.457951421029644	30.487794828973787	23.17870062378628
25	19.90705007594756	28.706254617968668	29.07074796672041	22.31594733936336
26	20.152883947185547	25.854581711608027	30.62073739174383	23.3717969494626
27	22.305235025421144	25.577984272125736	30.372980797490573	21.74379990496255
28	19.18795166823689	25.853483012742164	32.328664778735785	22.629900540285167
29	22.746362620067185	23.95493137252209	31.05966758865813	22.239038418752592
30	21.319427468020997	25.548594077463765	32.30724015085135	20.824738303663885
31	22.166524293605296	25.396149609824565	29.500339223274835	22.9369868732953
32	23.73519159934847	28.254964058813346	27.5490500374931	20.46079430434508
33	21.120288298582405	25.416475538843127	27.84899482787509	25.614241334699383
34	22.405765971648076	26.563517154809418	28.562599741256417	22.46811713228609
35	23.062238544004263	24.487525647751653	28.86419257993721	23.586043228306877
36	22.097580939772072	25.577160247976334	29.057288905613525	23.267969906638065
37	20.09520225672747	27.431214584128742	31.321981942884143	21.151601216259643
38	19.8350853002332	26.367124732535498	28.46014607201422	25.337643895217088
39	21.44440446401349	26.823908786020155	27.728961976778997	24.002724773187353
40	22.1431769427056	24.425998511263035	29.703323838743966	23.727500707287394
41	20.546767490599258	26.222371156957376	28.068734601048707	25.16212675139466
42	22.419774382187892	24.464178296851955	30.351830844322613	22.764216476637543
43	22.296170759777734	23.95136060120802	29.24461706224404	24.50785157677021
44	21.078537741679416	24.781427594371365	30.34139320509686	23.798641458852355
45	21.207360183702452	23.81924206258738	29.201493131758717	25.771904621951453
46	23.675037836442193	25.79360392455235	28.474978506703437	22.05637973230202
47	19.98066289996072	25.066814624780605	32.732436611942305	22.22008586331637
48	20.981577566766557	24.867675455342013	29.528905393787408	24.621841584104025
49	20.821442207066283	23.363282033252123	31.929013066276262	23.886262693405335
50	20.32043552423043	23.04960350704678	30.912716615348273	25.717244353374518
51	20.54841553889806	22.7054360873136	28.958680682401866	27.787467691386475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	250.0
1	484.0
2	718.0
3	3165.5
4	5613.0
5	3612.5
6	1612.0
7	1550.5
8	1489.0
9	1477.5
10	1466.0
11	1453.0
12	1440.0
13	1425.5
14	1411.0
15	1355.0
16	1299.0
17	1228.0
18	1157.0
19	1220.5
20	1284.0
21	1360.0
22	1436.0
23	1622.0
24	1808.0
25	2252.0
26	3129.0
27	3562.0
28	4189.0
29	4816.0
30	5515.5
31	6215.0
32	7026.0
33	7837.0
34	8510.5
35	9184.0
36	10150.5
37	11117.0
38	12136.0
39	13155.0
40	15003.5
41	16852.0
42	18232.0
43	19612.0
44	21287.5
45	22963.0
46	32210.0
47	41457.0
48	38630.0
49	35803.0
50	34390.5
51	32978.0
52	28252.0
53	23526.0
54	21254.0
55	18982.0
56	17306.5
57	15631.0
58	14404.5
59	13178.0
60	12365.5
61	11553.0
62	10205.0
63	8857.0
64	8031.0
65	7205.0
66	6057.0
67	4909.0
68	3991.5
69	3074.0
70	2907.5
71	2741.0
72	2471.0
73	2201.0
74	1728.0
75	941.0
76	627.0
77	538.0
78	449.0
79	435.0
80	421.0
81	257.0
82	93.0
83	91.5
84	90.0
85	52.5
86	15.0
87	20.0
88	25.0
89	14.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	364067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.02591501864329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34227539423125	25.338543902136333
2	8.670339823054698	6.073731716501451
3	3.4183081510044913	3.591881124138569
4	2.079817741986615	2.91390077940359
5	1.6558438384955174	2.899872278564405
6	1.3697606968428948	2.8786273058098115
7	1.2332564357392333	3.0237154580078105
8	1.1450980896613205	3.2086486701190546
9	0.9737354209052052	3.0695376692941716
>10	7.002325061487172	37.88690788541769
>50	0.06601514470285798	1.6094847904813907
>100	0.03379346693122492	2.08459523145469
>500	0.005501262058571498	1.2665023979827055
>1k	0.003929472898979642	4.154050790688331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3684	1.0119016554645162	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	3568	0.9800393883543413	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	3309	0.9088986367893822	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	3121	0.8572597900935817	Illumina PCR Primer Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1409	0.38701667550203667	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	836	0.229628062966432	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	754	0.20710473621613604	No Hit
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	716	0.19666709699038915	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	639	0.17551714382242828	Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	570	0.15656458838620363	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	568	0.1560152389532696	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	518	0.14228150312991839	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	462	0.12689971900776503	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.493494329340479E-4	0.0	0.0	0.18101063815176877	0.0
2	5.493494329340479E-4	0.0	0.0	0.9599881340522486	0.0
3	5.493494329340479E-4	0.0	0.0	1.3008594571878254	0.0
4	5.493494329340479E-4	0.0	0.0	1.9004743632353385	0.0
5	5.493494329340479E-4	0.0	0.0	4.017667077763159	0.0
6	5.493494329340479E-4	0.0	0.0	4.848832769792374	0.0
7	5.493494329340479E-4	0.0	0.0	5.843429918119467	0.0
8	5.493494329340479E-4	0.0	0.0	7.167911400923456	0.0
9	5.493494329340479E-4	0.0	0.0	7.691990759942538	0.0
10	5.493494329340479E-4	0.0	0.0	9.658112380413495	0.0
11	5.493494329340479E-4	0.0	0.0	11.070489772486932	0.0
12	5.493494329340479E-4	0.0	0.0	13.020405584686335	0.0
13	5.493494329340479E-4	0.0	0.0	13.485429879665007	0.0
14	5.493494329340479E-4	0.0	0.0	13.674955434027254	0.0
15	5.493494329340479E-4	0.0	0.0	14.103722666432278	0.0
16	5.493494329340479E-4	0.0	0.0	14.72641024866302	0.0
17	5.493494329340479E-4	0.0	0.0	15.502091647965896	0.0
18	5.493494329340479E-4	0.0	0.0	16.331883966412775	0.0
19	5.493494329340479E-4	0.0	0.0	16.94330988526837	0.0
20	5.493494329340479E-4	0.0	0.0	17.42975880813147	0.0
21	5.493494329340479E-4	0.0	0.0	17.9409284554766	0.0
22	5.493494329340479E-4	0.0	0.0	18.543290108688787	0.0
23	5.493494329340479E-4	0.0	0.0	19.093463565772232	0.0
24	5.493494329340479E-4	0.0	0.0	19.555741113586237	0.0
25	5.493494329340479E-4	0.0	0.0	19.949075307567014	0.0
26	5.493494329340479E-4	0.0	0.0	20.314118005751688	0.0
27	5.493494329340479E-4	0.0	0.0	20.70058533182079	0.0
28	5.493494329340479E-4	0.0	0.0	21.094194200518036	0.0
29	5.493494329340479E-4	0.0	0.0	21.513622492563183	0.0
30	5.493494329340479E-4	0.0	0.0	21.947059195148146	0.0
31	5.493494329340479E-4	0.0	0.0	22.366212812476824	0.0
32	8.240241494010718E-4	0.0	0.0	22.75158143968006	0.0
33	8.240241494010718E-4	0.0	0.0	23.12596307822461	0.0
34	8.240241494010718E-4	0.0	0.0	23.52396674238533	0.0
35	8.240241494010718E-4	0.0	0.0	23.948613854043348	0.0
36	8.240241494010718E-4	0.0	0.0	24.342772072173528	0.0
37	0.0010986988658680957	0.0	0.0	24.752586749142328	0.0
38	0.0010986988658680957	0.0	0.0	25.124770989955145	0.0
39	0.0010986988658680957	0.0	0.0	25.510688966591314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	20	7.0269307E-4	45.0	16
TTGTGCG	50	2.1827873E-11	45.0	1
CACGGCC	20	7.0269307E-4	45.0	17
AACTTCG	25	3.885537E-5	45.0	11
CGATTCG	20	7.0269307E-4	45.0	10
CGTTCAT	20	7.0269307E-4	45.0	17
CCCGTTA	40	6.7921064E-9	45.0	40
AGGTTCG	20	7.0269307E-4	45.0	43
GGAACGA	20	7.0269307E-4	45.0	8
GCGATTC	25	3.885537E-5	45.0	9
CGTAAGG	35	1.208864E-7	45.0	2
ACCCGTT	35	1.208864E-7	45.0	39
CGCAATT	25	3.885537E-5	45.0	20
CGGTAGT	25	3.885537E-5	45.0	12
CGTCATA	20	7.0269307E-4	45.0	38
CCGTTAC	20	7.0269307E-4	45.0	41
CCGTTAA	20	7.0269307E-4	45.0	41
CCGCAAT	20	7.0269307E-4	45.0	19
AGGTCGG	20	7.0269307E-4	45.0	2
CGGACCT	20	7.0269307E-4	45.0	21
>>END_MODULE