FastQCFastQC Report
Sat 18 Jun 2016
SRR3551957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629598
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146642.3291052385808086No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC26910.42741558899488247No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG26360.4186798560351208No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC21690.3445055416313267No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC10940.1737616701450767No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC8580.13627743417228136No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7500.1191236312694767No Hit
GAACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCT7010.1113408873598709No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG207.030852E-445.0000041
TAAGACG207.030852E-445.0000041
CGTTTTT55150.042.6337281
CCGTCGA650.041.53846441
CGGTCTA603.6379788E-1241.25000431
GTCGAAT556.002665E-1140.90909243
AACGGGA2050.039.5121964
TACGAAT403.4548975E-739.37500412
CGAATAT403.4548975E-739.37500414
CATACGA1100.038.8636418
GCAAACG356.2437866E-638.571431
TTAAACG356.2437866E-638.571431
ATGCGAG356.2437866E-638.571431
CGATGAA4400.038.35227619
TATTAGT1000.038.25000430
GTACGAG659.094947E-1238.0769271
CGGCGAA950.037.89473731
CGCATCG301.1393352E-437.50000421
GTTAGCG301.1393352E-437.5000041
CGTTCGG601.546141E-1037.50000445