##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629598
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32394162624405	33.0	31.0	34.0	30.0	34.0
2	32.44331144635148	34.0	31.0	34.0	31.0	34.0
3	32.398862448737134	34.0	31.0	34.0	30.0	34.0
4	35.96956152973802	37.0	35.0	37.0	35.0	37.0
5	36.01562743210747	37.0	35.0	37.0	35.0	37.0
6	35.999374203857066	37.0	35.0	37.0	35.0	37.0
7	36.15218917467972	37.0	36.0	37.0	35.0	37.0
8	35.99476491348448	37.0	36.0	37.0	35.0	37.0
9	37.89045708531476	39.0	38.0	39.0	35.0	39.0
10	37.628362860110734	39.0	37.0	39.0	35.0	39.0
11	37.5791981550132	39.0	37.0	39.0	35.0	39.0
12	37.511405690615284	39.0	37.0	39.0	35.0	39.0
13	37.50440280941172	39.0	37.0	39.0	35.0	39.0
14	38.82399721727197	40.0	38.0	41.0	35.0	41.0
15	38.866413171579325	40.0	38.0	41.0	35.0	41.0
16	38.87248371182882	40.0	38.0	41.0	35.0	41.0
17	38.802340541107185	40.0	38.0	41.0	35.0	41.0
18	38.622627454343885	40.0	38.0	41.0	35.0	41.0
19	38.442293336382896	40.0	37.0	41.0	35.0	41.0
20	38.2258997010791	40.0	36.0	41.0	34.0	41.0
21	38.138264416341855	40.0	36.0	41.0	34.0	41.0
22	38.15278002789081	40.0	36.0	41.0	34.0	41.0
23	38.09818963846772	40.0	36.0	41.0	34.0	41.0
24	38.02849913754491	40.0	35.0	41.0	34.0	41.0
25	37.858395357037345	40.0	35.0	41.0	34.0	41.0
26	37.854654875015484	40.0	35.0	41.0	34.0	41.0
27	37.83379076807741	40.0	35.0	41.0	34.0	41.0
28	37.72323450836883	40.0	35.0	41.0	33.0	41.0
29	37.63485906880263	40.0	35.0	41.0	33.0	41.0
30	37.44592581297908	40.0	35.0	41.0	33.0	41.0
31	37.31409089609561	40.0	35.0	41.0	33.0	41.0
32	37.0439312068971	40.0	35.0	41.0	32.0	41.0
33	36.77585062214302	40.0	35.0	41.0	31.0	41.0
34	36.532590637200244	40.0	35.0	41.0	31.0	41.0
35	36.32137967401421	39.0	35.0	41.0	30.0	41.0
36	36.19996886902436	39.0	35.0	41.0	29.0	41.0
37	36.107241446129116	39.0	35.0	41.0	29.0	41.0
38	35.98047801930756	39.0	35.0	41.0	28.0	41.0
39	35.90904196010788	39.0	35.0	41.0	28.0	41.0
40	35.8081283612718	39.0	35.0	41.0	27.0	41.0
41	35.70042789208352	39.0	35.0	41.0	27.0	41.0
42	35.580411627737064	39.0	35.0	41.0	26.0	41.0
43	35.46303990800479	39.0	35.0	41.0	26.0	41.0
44	35.47353390576209	39.0	35.0	41.0	26.0	41.0
45	35.438495675018025	38.0	35.0	41.0	26.0	41.0
46	35.3648185032354	38.0	35.0	41.0	26.0	41.0
47	35.30063786733757	38.0	35.0	41.0	26.0	41.0
48	35.24506907582298	38.0	35.0	40.0	26.0	41.0
49	35.18760860104384	38.0	35.0	40.0	26.0	41.0
50	35.07926327593162	37.0	35.0	40.0	26.0	41.0
51	34.29736593826537	36.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	6.0
11	13.0
12	22.0
13	27.0
14	30.0
15	33.0
16	63.0
17	115.0
18	188.0
19	359.0
20	647.0
21	1114.0
22	1776.0
23	2585.0
24	3778.0
25	5701.0
26	8802.0
27	10791.0
28	10606.0
29	9831.0
30	10052.0
31	10732.0
32	12959.0
33	17263.0
34	31571.0
35	46071.0
36	48540.0
37	66792.0
38	114343.0
39	214502.0
40	275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.600030495649605	19.023249756193636	21.86125114755765	19.51546860059911
2	37.46533502330058	24.554874697823056	21.86966921750069	16.110121061375672
3	30.01963157443321	23.932731679579668	30.38748534779335	15.660151398193767
4	26.672575198777633	23.646676133024567	32.0147141509344	17.666034517263395
5	25.440360356926167	27.479915755767966	28.66098685192774	18.41873703537813
6	24.27183694992678	33.689592406583245	27.91431993113066	14.124250712359315
7	72.59616453673614	6.205388200089581	16.798655650113247	4.399791613061033
8	73.76643509032748	7.287983761066585	14.56040203431396	4.385179114291977
9	67.9649554159956	7.68077408123914	16.419207176642875	7.935063326122382
10	38.29189419280239	23.952109123599502	22.693687082868752	15.062309600729353
11	28.73246103068943	23.06455865488772	29.2168971311853	18.98608318323756
12	24.93861162201913	20.779926238647516	33.33714528953396	20.944316849799396
13	24.27596656914412	20.73084730256449	35.37606536234232	19.617120765949068
14	20.83599376109835	24.53263828665275	33.17847261268302	21.45289533956588
15	19.906988268704794	22.81741682788065	36.09779573632699	21.17779916708757
16	22.353469991963127	21.860139326999136	33.704363736860664	22.082026944177077
17	21.754834036956915	23.147468702251278	32.40988694373235	22.687810317059455
18	22.076626672892864	22.75785501224591	32.93514274187656	22.230375572984666
19	23.583302361189205	23.384286481214996	31.062519258320386	21.96989189927541
20	24.413514655383274	23.298517466700975	31.80489772839177	20.483070149523982
21	23.74118088049835	24.638896565745128	31.32586189918011	20.294060654576413
22	23.163669516103926	21.714173170816935	31.309502253819105	23.812655059260035
23	23.30820618871089	23.62872817257996	31.186407834840647	21.8766578038685
24	22.132376532326976	24.212910460325475	31.34460401716651	22.310108990181035
25	22.769608543864496	25.547253962051975	30.13605507006058	21.54708242402295
26	21.78802982220401	24.582352548769215	31.13113446993161	22.49848315909517
27	23.648740942633236	23.806142967417305	30.63446834329207	21.910647746657393
28	21.73974504366278	25.11809122646514	31.806486043475363	21.335677686396718
29	22.602676628578873	24.63810240820333	30.265185086356688	22.49403587686111
30	22.28247230772652	26.034866692715035	29.86207071814078	21.82059028141767
31	23.179552666939855	25.713232888287447	27.843004583877327	23.264209860895367
32	23.204965708277346	26.309486370668267	28.32712302135649	22.158424899697906
33	22.807092779837294	26.172414778954188	26.831247875628577	24.189244565579944
34	21.553435684357318	26.822194479652094	27.72340445808278	23.900965377907806
35	22.87396084485656	25.433689433575076	27.548848630395906	24.14350109117246
36	22.907474293120373	28.737225975940202	25.780577447831792	22.574722283107633
37	22.40969634592232	27.593639115753227	27.12810396475211	22.868560573572346
38	21.686059993837336	27.62699373250868	26.958630745332734	23.728315528321247
39	23.082506615332324	25.467997039380684	27.471338854316564	23.97815749097043
40	22.162554518915243	25.094266500211248	29.20720840917538	23.53597057169813
41	21.60585008211589	25.727051229514707	28.16813268148882	24.498966006880583
42	21.877769624427014	26.28089669916359	28.5510754481431	23.290258228266293
43	23.275645729497235	24.782480249301937	28.39176744525872	23.55010657594211
44	22.84251220620142	24.98912004167739	28.803141051909314	23.36522670021188
45	22.27055994459957	24.316150940759023	28.739290785548878	24.67399832909253
46	22.72545338454061	25.735469299457748	28.47737762826438	23.061699687737256
47	21.862680631132882	25.39398155648525	30.38812067382679	22.355217138555076
48	21.824878732143368	25.50103399311942	28.722454645662786	23.951632629074425
49	21.664617740208833	25.133656714284353	30.292186442777773	22.909539102729042
50	21.538029028046466	24.49023027392082	30.147173275645727	23.824567422386984
51	21.74927493416434	24.78359206986045	28.69958290845905	24.76755008751616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	444.0
1	986.0
2	1528.0
3	11243.0
4	20958.0
5	13171.0
6	5384.0
7	5206.5
8	5029.0
9	4884.0
10	4739.0
11	4570.0
12	4401.0
13	4233.0
14	4065.0
15	3874.0
16	3683.0
17	3432.0
18	3181.0
19	2949.0
20	2717.0
21	2745.0
22	2773.0
23	2840.5
24	2908.0
25	3559.0
26	4831.5
27	5453.0
28	6461.0
29	7469.0
30	8179.0
31	8889.0
32	9682.5
33	10476.0
34	11304.0
35	12132.0
36	13497.5
37	14863.0
38	16069.0
39	17275.0
40	19384.0
41	21493.0
42	23202.5
43	24912.0
44	27947.5
45	30983.0
46	40034.0
47	49085.0
48	48827.5
49	48570.0
50	46754.5
51	44939.0
52	41926.5
53	38914.0
54	36441.0
55	33968.0
56	32504.0
57	31040.0
58	31003.5
59	30967.0
60	30114.5
61	29262.0
62	27606.5
63	25951.0
64	23807.0
65	21663.0
66	18586.5
67	15510.0
68	13603.5
69	11697.0
70	10575.0
71	9453.0
72	8261.0
73	7069.0
74	5942.0
75	3941.5
76	3068.0
77	2474.5
78	1881.0
79	1438.5
80	996.0
81	759.0
82	522.0
83	330.0
84	138.0
85	105.0
86	72.0
87	46.5
88	21.0
89	22.0
90	23.0
91	12.0
92	1.0
93	4.5
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.328510472316516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.94146626291571	22.934319350021592
2	7.405038124625593	4.78787705119723
3	3.347686546872667	3.2467715876581837
4	2.3091849402177465	2.9861003808938005
5	1.8284014631135912	2.9554747923933284
6	1.5737797299194642	3.052677268789251
7	1.4959839036435794	3.3854051906750877
8	1.315293676473991	3.4017188315248887
9	1.2612913870758864	3.669810463415288
>10	8.422036929811387	41.015885951952455
>50	0.05930912989951092	1.4215743944417631
>100	0.0326200214443591	2.350542982630231
>500	0.005436670240726516	1.076286964244255
>1k	0.0019769709966278243	1.3725215295216968
>5k	0.0	0.0
>10k+	4.942427491569561E-4	2.343033260640997
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14664	2.3291052385808086	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	2691	0.42741558899488247	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	2636	0.4186798560351208	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	2169	0.3445055416313267	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	1094	0.1737616701450767	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCC	858	0.13627743417228136	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	750	0.1191236312694767	No Hit
GAACTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCT	701	0.1113408873598709	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1766301671860457E-4	0.0	0.0	0.08815148713941277	0.0
2	3.1766301671860457E-4	0.0	0.0	0.38802537492177547	0.0
3	3.1766301671860457E-4	0.0	0.0	0.590059053554808	0.0
4	3.1766301671860457E-4	0.0	0.0	0.9197932649087195	0.0
5	3.1766301671860457E-4	0.0	0.0	1.9305969841073192	0.0
6	3.1766301671860457E-4	0.0	0.0	2.648674233399725	0.0
7	3.1766301671860457E-4	0.0	0.0	3.2090317948913434	0.0
8	3.1766301671860457E-4	0.0	0.0	4.003665831212933	0.0
9	3.1766301671860457E-4	0.0	0.0	4.423298676298209	0.0
10	3.1766301671860457E-4	0.0	0.0	5.494140705656625	0.0
11	3.1766301671860457E-4	0.0	0.0	6.263838195165804	0.0
12	3.1766301671860457E-4	0.0	0.0	7.178866514823745	0.0
13	3.1766301671860457E-4	0.0	0.0	7.470957658696501	0.0
14	3.1766301671860457E-4	0.0	0.0	7.622482917671276	0.0
15	3.1766301671860457E-4	0.0	0.0	7.857871213059762	0.0
16	3.1766301671860457E-4	0.0	0.0	8.169180969443994	0.0
17	3.1766301671860457E-4	0.0	0.0	8.519086782359537	0.0
18	3.1766301671860457E-4	0.0	0.0	8.91695971079959	0.0
19	3.1766301671860457E-4	0.0	0.0	9.220804386290935	0.0
20	3.1766301671860457E-4	0.0	0.0	9.459369311846606	0.0
21	4.764945250779069E-4	0.0	0.0	9.728112223990546	0.0
22	4.764945250779069E-4	0.0	0.0	10.022268177471974	0.0
23	4.764945250779069E-4	0.0	0.0	10.307847229502	0.0
24	4.764945250779069E-4	0.0	0.0	10.548635796174702	0.0
25	4.764945250779069E-4	0.0	0.0	10.770841076369367	0.0
26	4.764945250779069E-4	0.0	0.0	10.980657498912004	0.0
27	4.764945250779069E-4	0.0	0.0	11.196033024247217	0.0
28	4.764945250779069E-4	0.0	0.0	11.419985451033835	0.0
29	4.764945250779069E-4	0.0	0.0	11.64187306821178	0.0
30	4.764945250779069E-4	0.0	0.0	11.907598181696892	0.0
31	4.764945250779069E-4	0.0	0.0	12.124085527590621	0.0
32	4.764945250779069E-4	0.0	0.0	12.371227354597696	0.0
33	4.764945250779069E-4	0.0	0.0	12.590097173116813	0.0
34	4.764945250779069E-4	0.0	0.0	12.813890768395071	0.0
35	4.764945250779069E-4	0.0	0.0	13.060079606351989	0.0
36	4.764945250779069E-4	0.0	0.0	13.282920212580091	0.0
37	4.764945250779069E-4	0.0	0.0	13.514178888751236	0.0
38	4.764945250779069E-4	0.0	0.0	13.738766641571289	0.0
39	4.764945250779069E-4	0.0	0.0	13.961924910816109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	20	7.030852E-4	45.000004	1
TAAGACG	20	7.030852E-4	45.000004	1
CGTTTTT	5515	0.0	42.633728	1
CCGTCGA	65	0.0	41.538464	41
CGGTCTA	60	3.6379788E-12	41.250004	31
GTCGAAT	55	6.002665E-11	40.909092	43
AACGGGA	205	0.0	39.512196	4
TACGAAT	40	3.4548975E-7	39.375004	12
CGAATAT	40	3.4548975E-7	39.375004	14
CATACGA	110	0.0	38.86364	18
GCAAACG	35	6.2437866E-6	38.57143	1
TTAAACG	35	6.2437866E-6	38.57143	1
ATGCGAG	35	6.2437866E-6	38.57143	1
CGATGAA	440	0.0	38.352276	19
TATTAGT	100	0.0	38.250004	30
GTACGAG	65	9.094947E-12	38.076927	1
CGGCGAA	95	0.0	37.894737	31
CGCATCG	30	1.1393352E-4	37.500004	21
GTTAGCG	30	1.1393352E-4	37.500004	1
CGTTCGG	60	1.546141E-10	37.500004	45
>>END_MODULE