FastQCFastQC Report
Sat 18 Jun 2016
SRR3551956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587372
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.6074514958152585No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG18060.30747124479886684No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC16630.28312551500582256TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC16300.27750726966896616No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC14450.24601104581083197No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC8090.13773213568232737No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT6930.11798315207398379Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC6550.11151365744366433TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6160.10487391295465225No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA207.0304656E-445.0000048
GCGATTC207.0304656E-445.0000049
TTACGCG253.8884675E-545.0000041
CGGTCTA302.1636824E-645.00000431
CTAAGCG351.2105738E-745.01
TACGAAT1100.042.95454412
CACAACG750.042.012
CGAATAT1150.041.08695614
TACGCGG501.0804797E-940.5000042
CGTTTTT16800.040.446431
CGGGTAC451.9255822E-840.06
CATCGTT451.9255822E-840.036
GCTACGA1200.039.37500410
TAACGGG1100.038.8636363
TCGTTTA356.2430736E-638.57142638
TAACGCC356.2430736E-638.57142612
GTTCGAT356.2430736E-638.57142639
ATAACGC356.2430736E-638.57142611
GCCCGAA356.2430736E-638.57142625
ATCGTGA301.1392423E-437.50000430