##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587372
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.271131412460925	33.0	31.0	34.0	30.0	34.0
2	32.34202175112195	34.0	31.0	34.0	30.0	34.0
3	32.3554663824629	34.0	31.0	34.0	30.0	34.0
4	35.9093657852264	37.0	35.0	37.0	35.0	37.0
5	35.982915426680194	37.0	35.0	37.0	35.0	37.0
6	35.969639342699345	37.0	35.0	37.0	35.0	37.0
7	36.146145883698914	37.0	36.0	37.0	35.0	37.0
8	35.95535367705645	37.0	35.0	37.0	35.0	37.0
9	37.857928195419596	39.0	38.0	39.0	35.0	39.0
10	37.55915842089851	39.0	37.0	39.0	35.0	39.0
11	37.525076101686835	39.0	37.0	39.0	35.0	39.0
12	37.449030597304606	39.0	37.0	39.0	35.0	39.0
13	37.42140585523314	39.0	37.0	39.0	35.0	39.0
14	38.6663187894554	40.0	38.0	41.0	35.0	41.0
15	38.73245404956314	40.0	38.0	41.0	35.0	41.0
16	38.740626042780384	40.0	38.0	41.0	35.0	41.0
17	38.68714885966644	40.0	38.0	41.0	35.0	41.0
18	38.593153912682254	40.0	38.0	41.0	35.0	41.0
19	38.51756297542273	40.0	37.0	41.0	35.0	41.0
20	38.43242442608773	40.0	37.0	41.0	34.0	41.0
21	38.337178142642145	40.0	37.0	41.0	34.0	41.0
22	38.357568968217755	40.0	37.0	41.0	34.0	41.0
23	38.3115946963764	40.0	37.0	41.0	34.0	41.0
24	38.227758558460394	40.0	37.0	41.0	34.0	41.0
25	38.05356911803763	40.0	36.0	41.0	34.0	41.0
26	38.098258003445856	40.0	37.0	41.0	34.0	41.0
27	38.09173743385793	40.0	37.0	41.0	34.0	41.0
28	38.02172047697201	40.0	36.0	41.0	34.0	41.0
29	37.974014764067746	40.0	36.0	41.0	34.0	41.0
30	37.79946269144597	40.0	36.0	41.0	33.0	41.0
31	37.76643592135819	40.0	36.0	41.0	33.0	41.0
32	37.645109062059475	40.0	36.0	41.0	33.0	41.0
33	37.53387291188548	40.0	36.0	41.0	33.0	41.0
34	37.4145737284038	40.0	36.0	41.0	33.0	41.0
35	37.30968960045763	40.0	35.0	41.0	33.0	41.0
36	37.21470720429302	40.0	35.0	41.0	33.0	41.0
37	37.1906832467329	40.0	35.0	41.0	33.0	41.0
38	37.0728465095374	40.0	35.0	41.0	32.0	41.0
39	37.018599797062166	40.0	35.0	41.0	32.0	41.0
40	36.91084866149561	39.0	35.0	41.0	32.0	41.0
41	36.82770033300872	39.0	35.0	41.0	31.0	41.0
42	36.717386937068845	39.0	35.0	41.0	31.0	41.0
43	36.57542579489659	39.0	35.0	41.0	31.0	41.0
44	36.60717398854559	39.0	35.0	41.0	31.0	41.0
45	36.557478054793215	39.0	35.0	41.0	31.0	41.0
46	36.4542657804594	39.0	35.0	41.0	31.0	41.0
47	36.37290507548879	39.0	35.0	41.0	31.0	41.0
48	36.32086309868363	39.0	35.0	41.0	31.0	41.0
49	36.286081052552724	38.0	35.0	41.0	31.0	41.0
50	36.18212478633643	38.0	35.0	41.0	31.0	41.0
51	35.30031223824084	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	12.0
10	14.0
11	13.0
12	17.0
13	22.0
14	25.0
15	22.0
16	57.0
17	85.0
18	135.0
19	260.0
20	471.0
21	711.0
22	1123.0
23	1624.0
24	2312.0
25	3451.0
26	4670.0
27	5594.0
28	5873.0
29	6574.0
30	7514.0
31	9038.0
32	11765.0
33	15994.0
34	31071.0
35	44106.0
36	42605.0
37	63997.0
38	112279.0
39	215643.0
40	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.488160824826515	19.0456814420844	23.089115586034065	13.377042147055018
2	33.511982185054784	25.24992679256076	23.595438665785906	17.642652356598543
3	32.80425352246958	24.067882023657923	26.60358341902576	16.52428103484674
4	28.88986877140892	24.720790231744108	27.337700809708327	19.051640187138645
5	25.708239412161287	28.538302813208666	24.547135375877637	21.20632239875241
6	25.97825568804778	34.479852631722316	24.48686692590045	15.055024754329455
7	77.04061480628971	6.581859537056584	11.395504041731645	4.9820216149220595
8	76.71509707646943	7.568116968462917	10.237464502904462	5.4793214521631946
9	70.26467043032353	8.566462139836425	12.860333825922924	8.308533603917109
10	39.175173484606006	24.723173729765804	18.96685575750972	17.134797028118466
11	30.788835695266375	23.759729779424283	24.964928529109322	20.486505996200023
12	27.4420639730869	21.40687673229231	29.943374896998836	21.20768439762195
13	25.557227787500935	22.198708825071677	31.058341221576786	21.185722165850603
14	21.32924279672848	26.312796660378773	29.932649155901203	22.42531138699155
15	20.515107972460385	23.849281205096602	33.541435410608614	22.094175411834406
16	22.91256648256982	23.471496768657683	31.034335991501127	22.58160075727137
17	22.897754744863562	23.15721552951111	29.27429295233685	24.67073677328848
18	23.333253883399276	23.632382885122205	29.507024509169653	23.527338722308862
19	24.513766403573886	25.17229285699693	27.474070946521113	22.839869792908072
20	26.088066846904518	24.709553741070394	27.81661366221066	21.38576574981443
21	25.08206043188984	25.638607219956004	28.129532902487693	21.14979944566646
22	24.032640302908547	23.778287014021778	27.025462568866065	25.163610114203607
23	23.991780336822323	25.637585720803852	27.3475753015125	23.023058640861326
24	24.014934317604517	24.22791689082898	28.038789727804524	23.718359063761977
25	23.0628971077954	25.54258629965337	26.688878598230765	24.705637994320465
26	22.24110103988614	25.70551541442221	27.46402620485825	24.589357340833406
27	23.71529456630551	24.864310862621984	27.88011685950301	23.540277711569498
28	21.745163201514544	26.256103457434133	28.343877474581696	23.65485586646963
29	23.056938362741157	24.73849621704814	28.00201575832692	24.20254966188378
30	22.97692092915563	25.461036617339605	28.18860960345403	23.373432850050733
31	23.91499765055195	25.965316698787138	25.404343414394965	24.715342236265943
32	24.240004630796154	26.490537512853862	26.45018829634371	22.819269560006266
33	23.265834939357	26.26410520079268	25.951866959950426	24.518192899899894
34	22.546359036522	26.207922747424117	26.563574702232994	24.682143513820883
35	23.92351014348658	23.86817893941148	26.808734498750365	25.39957641835157
36	22.36231893927528	27.7856281879286	25.457631620165756	24.39442125263036
37	22.440804124132576	26.398943088877235	27.698971009854063	23.461281777136126
38	22.529674550370125	27.6381918103008	25.72526439803055	24.10686924129853
39	23.179177761282457	24.78871992536246	26.50773274858182	25.524369564773263
40	24.07622426673386	23.99109933738755	28.101782175520796	23.8308942203578
41	21.570146346778532	24.753648454471783	27.154001212178997	26.52220398657069
42	22.709288151290835	25.123260897693456	26.915140660433252	25.252310290582457
43	22.536484544717826	24.457924449922707	27.5019919233467	25.503599082012762
44	22.54686978609808	25.11389715546536	27.330550315643237	25.008682742793326
45	22.716268395497234	23.88282042725905	27.339062808577868	26.061848368665853
46	23.414633315854346	25.187104594703186	26.860490455792924	24.537771633649545
47	21.84901561531704	24.789741424514617	28.782100610856492	24.57914234931185
48	22.42582213656763	24.587654842246483	27.705781004201768	25.280742016984124
49	22.155635610822443	24.004549076224265	28.836921065355515	25.002894247597773
50	21.0496925287552	23.835661216401192	28.743624142791962	26.371022112051644
51	21.80219690417657	23.909549655073786	26.82337598659793	27.464877454151715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	463.0
1	852.0
2	1241.0
3	3553.0
4	5865.0
5	3930.5
6	1996.0
7	2311.0
8	2626.0
9	2345.5
10	2065.0
11	1959.0
12	1853.0
13	1858.5
14	1864.0
15	1828.0
16	1792.0
17	1757.5
18	1723.0
19	1635.0
20	1547.0
21	1767.5
22	1988.0
23	2371.5
24	2755.0
25	3033.5
26	3891.0
27	4470.0
28	5213.0
29	5956.0
30	6670.0
31	7384.0
32	8294.0
33	9204.0
34	10572.5
35	11941.0
36	12660.5
37	13380.0
38	15128.0
39	16876.0
40	19583.5
41	22291.0
42	24488.5
43	26686.0
44	30456.5
45	34227.0
46	41113.0
47	47999.0
48	49345.5
49	50692.0
50	49648.0
51	48604.0
52	44190.0
53	39776.0
54	36680.5
55	33585.0
56	32558.0
57	31531.0
58	30559.5
59	29588.0
60	29050.0
61	28512.0
62	26258.5
63	24005.0
64	21422.5
65	18840.0
66	16527.0
67	14214.0
68	12183.0
69	10152.0
70	9225.0
71	8298.0
72	7473.5
73	6649.0
74	5649.0
75	3889.0
76	3129.0
77	2478.5
78	1828.0
79	1461.0
80	1094.0
81	785.5
82	477.0
83	327.0
84	177.0
85	105.5
86	34.0
87	31.5
88	29.0
89	16.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	587372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.845452981953358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19759475080579	22.269675142961027
2	8.7572163085948	5.40240607799112
3	3.1988408238889003	2.96009082690054
4	1.7966519938065295	2.2167417839956824
5	1.3694692277026341	2.112094933666679
6	1.0999097635169097	2.0356328936971364
7	0.9183004635662033	1.9827775640566105
8	0.8536683554473972	2.1065429696107305
9	0.7715594613443235	2.1419190978909826
>10	8.887815559776978	49.63283513058899
>50	0.09171863099135392	1.9289336725997177
>100	0.05114008515881412	2.8225872105187455
>500	0.0033352229451400514	0.653951380204015
>1k	0.0027793524542833762	1.7338113153180388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3568	0.6074514958152585	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	1806	0.30747124479886684	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1663	0.28312551500582256	TruSeq Adapter, Index 13 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	1630	0.27750726966896616	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	1445	0.24601104581083197	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	809	0.13773213568232737	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	693	0.11798315207398379	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	655	0.11151365744366433	TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	616	0.10487391295465225	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12138814924783613	0.0
2	0.0	0.0	0.0	0.6706141933902194	0.0
3	0.0	0.0	0.0	0.9205409859509817	0.0
4	0.0	0.0	0.0	1.3328861437045008	0.0
5	0.0	0.0	0.0	2.5922243484537906	0.0
6	0.0	0.0	0.0	3.320382994082115	0.0
7	0.0	0.0	0.0	4.025728158645628	0.0
8	0.0	0.0	0.0	5.058463801475045	0.0
9	0.0	0.0	0.0	5.533460907227447	0.0
10	0.0	0.0	0.0	6.711760179239051	0.0
11	0.0	0.0	0.0	7.876609712413939	0.0
12	0.0	0.0	0.0	8.981020545752948	0.0
13	0.0	0.0	0.0	9.344674243920377	0.0
14	0.0	0.0	0.0	9.503687611939283	0.0
15	0.0	0.0	0.0	9.851508073248299	0.0
16	0.0	0.0	0.0	10.458959569063557	0.0
17	0.0	0.0	0.0	11.099609787323876	0.0
18	0.0	0.0	0.0	11.817212941713258	0.0
19	0.0	0.0	0.0	12.29799854266121	0.0
20	0.0	0.0	0.0	12.739796925968552	0.0
21	0.0	0.0	0.0	13.241523259535695	0.0
22	0.0	0.0	0.0	13.79670804873232	0.0
23	0.0	0.0	0.0	14.330611605592367	0.0
24	0.0	0.0	0.0	14.745680761084968	0.0
25	0.0	0.0	0.0	15.128402443425973	0.0
26	0.0	0.0	0.0	15.496823137636795	0.0
27	0.0	0.0	0.0	15.839025353608957	0.0
28	0.0	0.0	0.0	16.188037563928823	0.0
29	0.0	0.0	0.0	16.5436895187377	0.0
30	1.7024985869261729E-4	0.0	0.0	16.964887669143234	0.0
31	1.7024985869261729E-4	0.0	0.0	17.362931838766574	0.0
32	3.4049971738523457E-4	0.0	0.0	17.766083504150693	0.0
33	3.4049971738523457E-4	0.0	0.0	18.15953092758933	0.0
34	3.4049971738523457E-4	0.0	0.0	18.536974864310864	0.0
35	3.4049971738523457E-4	0.0	0.0	18.937913281531976	0.0
36	3.4049971738523457E-4	0.0	0.0	19.3386814488944	0.0
37	3.4049971738523457E-4	0.0	0.0	19.74030086555028	0.0
38	3.4049971738523457E-4	0.0	0.0	20.13579128729323	0.0
39	5.107495760778519E-4	0.0	0.0	20.49740198715635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACGTA	20	7.0304656E-4	45.000004	8
GCGATTC	20	7.0304656E-4	45.000004	9
TTACGCG	25	3.8884675E-5	45.000004	1
CGGTCTA	30	2.1636824E-6	45.000004	31
CTAAGCG	35	1.2105738E-7	45.0	1
TACGAAT	110	0.0	42.954544	12
CACAACG	75	0.0	42.0	12
CGAATAT	115	0.0	41.086956	14
TACGCGG	50	1.0804797E-9	40.500004	2
CGTTTTT	1680	0.0	40.44643	1
CGGGTAC	45	1.9255822E-8	40.0	6
CATCGTT	45	1.9255822E-8	40.0	36
GCTACGA	120	0.0	39.375004	10
TAACGGG	110	0.0	38.863636	3
TCGTTTA	35	6.2430736E-6	38.571426	38
TAACGCC	35	6.2430736E-6	38.571426	12
GTTCGAT	35	6.2430736E-6	38.571426	39
ATAACGC	35	6.2430736E-6	38.571426	11
GCCCGAA	35	6.2430736E-6	38.571426	25
ATCGTGA	30	1.1392423E-4	37.500004	30
>>END_MODULE