FastQCFastQC Report
Sat 18 Jun 2016
SRR3551954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338290
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93272.757101894823968No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG17850.5276537881699134No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC15640.4623252239203051No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC13710.40527358183806794No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC13250.39167578113452955RNA PCR Primer, Index 33 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC9580.2831889798693429No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT6630.19598569274882496TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC4880.14425492920275504Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC4220.12474504123680866RNA PCR Primer, Index 33 (95% over 24bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA3930.11617251470631706No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT3710.10966921871766828No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3670.10848680126518667No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC3460.10227910963965829No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3390.10020987909781548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGT207.0262206E-445.014
CGTATGG406.7902874E-945.02
CGCATCG207.0262206E-445.021
TAAACGG253.8849495E-545.02
GAATACG207.0262206E-445.021
GCCGCAT207.0262206E-445.019
TAACCGG207.0262206E-445.02
CGGATGG207.0262206E-445.02
GCCCTCG207.0262206E-445.013
AACGCCG207.0262206E-445.013
CGAATGC253.8849495E-545.045
GCGATCT207.0262206E-445.09
TTAACCG207.0262206E-445.01
GCGGCTA253.8849495E-545.024
CTCGAAT207.0262206E-445.043
ACGTATG207.0262206E-445.01
TAGTGAG253.8849495E-545.01
CCAGTCG207.0262206E-445.028
CGATAGA207.0262206E-445.010
TACACGG253.8849495E-545.02