##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338290
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29186792397056	33.0	31.0	34.0	30.0	34.0
2	32.409101658340475	34.0	31.0	34.0	31.0	34.0
3	32.36354311389636	34.0	31.0	34.0	30.0	34.0
4	35.95588696089154	37.0	35.0	37.0	35.0	37.0
5	36.044606698394865	37.0	35.0	37.0	35.0	37.0
6	36.04270891838364	37.0	35.0	37.0	35.0	37.0
7	36.22614029383074	37.0	37.0	37.0	35.0	37.0
8	36.04687102781637	37.0	37.0	37.0	35.0	37.0
9	37.99729817612108	39.0	39.0	39.0	35.0	39.0
10	37.72998019450767	39.0	37.0	39.0	35.0	39.0
11	37.6691743770138	39.0	37.0	39.0	35.0	39.0
12	37.59804014307251	39.0	37.0	39.0	35.0	39.0
13	37.55802713648053	39.0	37.0	39.0	35.0	39.0
14	38.828756392444355	40.0	38.0	41.0	35.0	41.0
15	38.888982825386506	40.0	38.0	41.0	35.0	41.0
16	38.91005054834609	40.0	38.0	41.0	35.0	41.0
17	38.86245233379645	40.0	38.0	41.0	35.0	41.0
18	38.64455644565314	40.0	38.0	41.0	35.0	41.0
19	38.425262348872266	40.0	37.0	41.0	35.0	41.0
20	38.17711726625085	40.0	35.0	41.0	34.0	41.0
21	38.115131987348136	40.0	35.0	41.0	34.0	41.0
22	38.133707765526616	40.0	35.0	41.0	35.0	41.0
23	38.09709420911053	40.0	35.0	41.0	35.0	41.0
24	38.01467675662893	40.0	35.0	41.0	34.0	41.0
25	37.879485057199446	40.0	35.0	41.0	34.0	41.0
26	37.9141624050371	40.0	35.0	41.0	34.0	41.0
27	37.9136510094889	40.0	35.0	41.0	34.0	41.0
28	37.83126607348724	40.0	35.0	41.0	34.0	41.0
29	37.75831387271276	40.0	35.0	41.0	34.0	41.0
30	37.51261935026161	40.0	35.0	41.0	33.0	41.0
31	37.30354725235745	40.0	35.0	41.0	33.0	41.0
32	36.99529102249549	40.0	35.0	41.0	33.0	41.0
33	36.67070560761477	40.0	35.0	41.0	31.0	41.0
34	36.33162375476662	40.0	35.0	41.0	30.0	41.0
35	36.1135564160927	40.0	35.0	41.0	29.0	41.0
36	35.98306778208046	39.0	35.0	41.0	27.0	41.0
37	35.887756067279554	39.0	35.0	41.0	27.0	41.0
38	35.78465813355405	39.0	35.0	41.0	26.0	41.0
39	35.71224688876408	39.0	35.0	41.0	25.0	41.0
40	35.60133021963404	39.0	35.0	41.0	24.0	41.0
41	35.48787135298117	39.0	35.0	41.0	24.0	41.0
42	35.42871205178989	39.0	35.0	41.0	23.0	41.0
43	35.3425345118094	39.0	35.0	41.0	23.0	41.0
44	35.36285731177392	39.0	35.0	41.0	23.0	41.0
45	35.3239676017618	39.0	35.0	41.0	23.0	41.0
46	35.230973425167754	39.0	35.0	41.0	23.0	41.0
47	35.163945135830204	38.0	35.0	41.0	23.0	41.0
48	35.15315262053268	38.0	35.0	41.0	23.0	41.0
49	35.155088829111115	38.0	35.0	41.0	24.0	41.0
50	35.04364598421473	38.0	35.0	41.0	24.0	41.0
51	34.27708770581454	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	2.0
12	10.0
13	10.0
14	10.0
15	17.0
16	25.0
17	42.0
18	90.0
19	159.0
20	288.0
21	524.0
22	803.0
23	1223.0
24	1976.0
25	3452.0
26	5408.0
27	6736.0
28	6538.0
29	5725.0
30	5499.0
31	5849.0
32	6762.0
33	9006.0
34	16946.0
35	25260.0
36	23387.0
37	31516.0
38	60563.0
39	120260.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.11939460226433	18.390138638446306	22.239203050637027	20.25126370865234
2	38.37388039847468	24.15531053238346	22.564367850069466	14.906441219072395
3	29.047267137662953	23.368411717756953	32.863223861184196	14.721097283395904
4	25.39803127494162	22.709213987998464	34.89668627508942	16.9960684619705
5	24.458009400218746	26.07230482721925	30.900115285701617	18.569570486860385
6	23.74885453309291	31.565816311448756	30.987318572822137	13.6980105826362
7	70.77891749682226	5.722309261284696	19.26719678382453	4.231576458068521
8	70.49425049513731	7.21185964704839	17.310000295604365	4.983889562209938
9	64.63832806172219	7.806320021283514	19.449880280232936	8.10547163676136
10	33.40211061515268	27.392178308551834	25.417836767270686	13.7878743090248
11	26.610304768098374	21.64119542404446	33.864140234709865	17.8843595731473
12	24.32144018445712	19.278725353986225	36.84324100623726	19.5565934553194
13	22.672559046971532	21.477726211238878	38.11345295456561	17.736261787223977
14	18.850394631824766	25.699843329687543	35.56179609211032	19.887965946377367
15	17.771734310798426	22.983830441337314	40.193029649117626	19.051405598746637
16	19.122646250258654	22.76656123444382	37.155103609329274	20.955688905968252
17	19.02361878861332	22.058884389133585	35.850897159242066	23.066599663011026
18	19.271039640545094	21.83215584262024	37.84918265393597	21.047621862898698
19	20.521150492181263	23.5776996068462	34.881906056933396	21.01924384403914
20	21.0810251559313	24.334742380797543	35.87691034319667	18.707322120074494
21	20.79960980224068	24.745928049898016	35.89021253953708	18.564249608324218
22	19.422093470099618	22.245115137899436	35.37556534334447	22.95722604865648
23	18.585828726832005	24.737946732093764	35.03089065594608	21.645333885128146
24	19.876437376215673	23.62322267876674	35.46335983919123	21.036980105826363
25	19.198616571580594	25.757190576132906	33.724910579680156	21.319282272606344
26	17.377693694758932	24.954624730261017	35.41340270182388	22.25427887315617
27	20.149871412102044	24.241627006414614	34.88072363948092	20.727777942002422
28	17.848887049572852	24.067220432173578	37.065831091666915	21.018061426586655
29	19.308285790298267	22.238020633184547	36.47432676106299	21.979366815454195
30	20.890655946081765	25.230127996689234	34.03529516095657	19.84392089627243
31	21.981436045996038	24.86121375151497	31.645038280765025	21.512311921723963
32	20.704720801679034	26.154778444529843	31.565225102722515	21.57527565106861
33	22.346211830086613	25.273581838067933	29.718880250672502	22.66132608117296
34	20.43512962251323	25.302846670016848	31.77717343107984	22.484850276390077
35	19.63551982027255	26.16187294924473	31.20577019716811	22.996837033314613
36	22.50022170327234	26.617694877176383	30.19362085784386	20.68846256170741
37	20.14070767684531	27.738035413402702	30.699695527505988	21.421561382246
38	21.313665789707056	26.965916816932218	28.140648555972685	23.57976883738804
39	21.667799816725296	25.129326908865174	29.313902273197552	23.888971001211978
40	20.809364746223654	26.10068284607881	31.361258092169443	21.728694315528095
41	18.414378196222174	26.89497176978332	30.0159626356085	24.674687398386
42	19.870229684590143	26.134972952200776	30.802861450235003	23.191935912974078
43	21.274350409412044	23.819799580241803	30.39197138549765	24.513878624848502
44	20.936770226728544	24.100032516479946	31.171184486683025	23.792012770108485
45	20.80670430695557	24.416920393745013	30.0449318631943	24.731443436105117
46	22.37665907948801	25.7223092612847	31.062106476691596	20.838925182535696
47	18.94262319311833	25.741227940524404	33.34476336870732	21.971385497649944
48	19.17999349670401	25.01581483342694	31.750864642762128	24.053327027106917
49	20.329007656153006	22.983239232611076	33.772798486505664	22.91495462473026
50	19.758786839693755	22.888350232049426	33.0009754943983	24.351887433858526
51	19.474415442371928	23.160306246120193	30.845428478524344	26.51984983298353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	292.0
1	497.0
2	702.0
3	7198.5
4	13695.0
5	8659.0
6	3623.0
7	3493.0
8	3363.0
9	3302.5
10	3242.0
11	3104.0
12	2966.0
13	2824.5
14	2683.0
15	2503.5
16	2324.0
17	2129.0
18	1934.0
19	1901.0
20	1868.0
21	1887.0
22	1906.0
23	1999.5
24	2093.0
25	2350.5
26	3075.0
27	3542.0
28	3975.0
29	4408.0
30	4688.5
31	4969.0
32	5849.0
33	6729.0
34	7113.5
35	7498.0
36	8106.0
37	8714.0
38	9429.5
39	10145.0
40	11553.0
41	12961.0
42	14756.5
43	16552.0
44	18368.0
45	20184.0
46	25963.5
47	31743.0
48	31650.0
49	31557.0
50	30219.0
51	28881.0
52	25340.0
53	21799.0
54	19123.0
55	16447.0
56	15167.5
57	13888.0
58	12678.0
59	11468.0
60	10734.5
61	10001.0
62	9100.5
63	8200.0
64	7787.0
65	7374.0
66	6317.0
67	5260.0
68	4555.0
69	3850.0
70	3295.5
71	2741.0
72	2645.5
73	2550.0
74	2008.5
75	1176.5
76	886.0
77	712.5
78	539.0
79	446.0
80	353.0
81	263.5
82	174.0
83	115.5
84	57.0
85	45.5
86	34.0
87	26.5
88	19.0
89	10.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	338290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.50586543316978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08650833019072	27.41097968948524
2	8.746185173297965	6.3857411798000046
3	3.207665524777883	3.51295818006478
4	1.7702255304228203	2.584944599999083
5	1.304676670559547	2.38141754846214
6	1.0048964011687698	2.2010767677206227
7	0.8829053652395848	2.256185711756193
8	0.8097734375081777	2.364918411282308
9	0.7317946328603971	2.4043316752725787
>10	6.327853480402607	38.57653068564762
>50	0.0812200341220089	2.015612191044923
>100	0.04061001706100445	2.866865883379429
>500	0.0016244006824401777	0.4806277378175669
>1k	0.0032488013648803555	1.7923471160439186
>5k	8.122003412200889E-4	2.7654626222235943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9327	2.757101894823968	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	1785	0.5276537881699134	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	1564	0.4623252239203051	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	1371	0.40527358183806794	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1325	0.39167578113452955	RNA PCR Primer, Index 33 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	958	0.2831889798693429	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	663	0.19598569274882496	TruSeq Adapter, Index 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	488	0.14425492920275504	Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	422	0.12474504123680866	RNA PCR Primer, Index 33 (95% over 24bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	393	0.11617251470631706	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	371	0.10966921871766828	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	367	0.10848680126518667	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	346	0.10227910963965829	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	339	0.10020987909781548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20367140618995536	0.0
2	0.0	0.0	0.0	0.9692867066717905	0.0
3	0.0	0.0	0.0	1.3086405155340093	0.0
4	0.0	0.0	0.0	1.8844778148925478	0.0
5	0.0	0.0	0.0	3.550208401076	0.0
6	0.0	0.0	0.0	4.309320405569186	0.0
7	0.0	0.0	0.0	5.144698335747436	0.0
8	0.0	0.0	0.0	6.388010287031837	0.0
9	0.0	0.0	0.0	6.898814626503887	0.0
10	0.0	0.0	0.0	8.509562801146945	0.0
11	0.0	0.0	0.0	10.145437346655237	0.0
12	0.0	0.0	0.0	11.796683319045789	0.0
13	0.0	0.0	0.0	12.27763161784268	0.0
14	0.0	0.0	0.0	12.490171154926246	0.0
15	0.0	0.0	0.0	12.957226048656478	0.0
16	0.0	0.0	0.0	13.653078719441899	0.0
17	0.0	0.0	0.0	14.477519288184693	0.0
18	0.0	0.0	0.0	15.39300600076857	0.0
19	0.0	0.0	0.0	16.007863076059003	0.0
20	0.0	0.0	0.0	16.539950929675722	0.0
21	0.0	0.0	0.0	17.14357503916758	0.0
22	0.0	0.0	0.0	17.746312335570074	0.0
23	0.0	0.0	0.0	18.340477105442076	0.0
24	0.0	0.0	0.0	18.818173756244644	0.0
25	0.0	0.0	0.0	19.253894587484112	0.0
26	0.0	0.0	0.0	19.67513080493068	0.0
27	0.0	0.0	0.0	20.101392296550298	0.0
28	0.0	0.0	0.0	20.553666972124507	0.0
29	2.9560436312039967E-4	0.0	0.0	20.96899110230867	0.0
30	2.9560436312039967E-4	0.0	0.0	21.4330899524077	0.0
31	2.9560436312039967E-4	0.0	0.0	21.870584409825888	0.0
32	2.9560436312039967E-4	0.0	0.0	22.298028318897988	0.0
33	2.9560436312039967E-4	0.0	0.0	22.705075526914776	0.0
34	2.9560436312039967E-4	0.0	0.0	23.118034822193977	0.0
35	2.9560436312039967E-4	0.0	0.0	23.574152354488753	0.0
36	2.9560436312039967E-4	0.0	0.0	23.997162198114044	0.0
37	2.9560436312039967E-4	0.0	0.0	24.431996216264153	0.0
38	2.9560436312039967E-4	0.0	0.0	24.842295072275267	0.0
39	2.9560436312039967E-4	0.0	0.0	25.280380738419698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	20	7.0262206E-4	45.0	14
CGTATGG	40	6.7902874E-9	45.0	2
CGCATCG	20	7.0262206E-4	45.0	21
TAAACGG	25	3.8849495E-5	45.0	2
GAATACG	20	7.0262206E-4	45.0	21
GCCGCAT	20	7.0262206E-4	45.0	19
TAACCGG	20	7.0262206E-4	45.0	2
CGGATGG	20	7.0262206E-4	45.0	2
GCCCTCG	20	7.0262206E-4	45.0	13
AACGCCG	20	7.0262206E-4	45.0	13
CGAATGC	25	3.8849495E-5	45.0	45
GCGATCT	20	7.0262206E-4	45.0	9
TTAACCG	20	7.0262206E-4	45.0	1
GCGGCTA	25	3.8849495E-5	45.0	24
CTCGAAT	20	7.0262206E-4	45.0	43
ACGTATG	20	7.0262206E-4	45.0	1
TAGTGAG	25	3.8849495E-5	45.0	1
CCAGTCG	20	7.0262206E-4	45.0	28
CGATAGA	20	7.0262206E-4	45.0	10
TACACGG	25	3.8849495E-5	45.0	2
>>END_MODULE