FastQCFastQC Report
Sat 18 Jun 2016
SRR3551951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391756
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC139333.5565505059271585No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC125853.212458775360173No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG114902.9329480595064275No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC37920.9679494379154372TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT35550.9074525980457224No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.8625266747669468No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT26230.6695494134103881No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT25800.6585731935184146No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA24790.6327918398186626No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC23470.599097397359581No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT9980.2547504058648751No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC8430.21518496206822615TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT7950.20293243753765097TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG7710.1968061752723634No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGCAGTA5440.13886194467985175No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT5430.13860668375213142No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT5080.12967255128192037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA302.1618253E-645.00000441
CGATCGA1150.045.00000441
GCATAAG207.0275884E-445.01
TCGCAGG453.8380676E-1045.02
CGAGTGG207.0275884E-445.029
ACCGCAG253.8860824E-545.023
CTCCATA207.0275884E-445.011
CACGATC207.0275884E-445.039
TGACGCG207.0275884E-445.01
TAGCACG207.0275884E-445.01
ACGATCG502.1827873E-1145.040
TGCGATG207.0275884E-445.01
TACGACC207.0275884E-445.06
AACGCGA207.0275884E-445.025
CGTAAGC453.8380676E-1045.043
CACCGTA253.8860824E-545.040
TAGCTAG207.0275884E-445.01
GCACTAC207.0275884E-445.09
AAATGCG207.0275884E-445.01
CCGACTT551.8189894E-1244.99999644