##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391756
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970956411643982	33.0	31.0	34.0	30.0	34.0
2	32.07444174435108	33.0	31.0	34.0	30.0	34.0
3	32.086655979742496	33.0	31.0	34.0	30.0	34.0
4	35.7485220392285	37.0	35.0	37.0	35.0	37.0
5	35.750497758809054	37.0	35.0	37.0	35.0	37.0
6	35.638239108016215	37.0	35.0	37.0	35.0	37.0
7	35.81747056841503	37.0	35.0	37.0	35.0	37.0
8	35.07814813302157	37.0	35.0	37.0	32.0	37.0
9	36.72751406487712	39.0	37.0	39.0	32.0	39.0
10	36.56735570099756	38.0	35.0	39.0	32.0	39.0
11	36.995060701048615	39.0	37.0	39.0	34.0	39.0
12	37.06224027200604	39.0	37.0	39.0	34.0	39.0
13	37.09269545329236	39.0	37.0	39.0	33.0	39.0
14	38.22332268044395	40.0	38.0	41.0	33.0	41.0
15	38.13023667793218	40.0	37.0	41.0	33.0	41.0
16	38.389124863435406	40.0	38.0	41.0	34.0	41.0
17	38.13086206720509	40.0	37.0	41.0	33.0	41.0
18	38.052619487640264	40.0	37.0	41.0	34.0	41.0
19	37.96345939819684	40.0	37.0	41.0	34.0	41.0
20	37.638741972043825	40.0	36.0	41.0	32.0	41.0
21	37.84257548065633	40.0	36.0	41.0	33.0	41.0
22	37.8490616608297	40.0	36.0	41.0	33.0	41.0
23	37.71189975392846	40.0	36.0	41.0	33.0	41.0
24	37.68676165776657	40.0	36.0	41.0	33.0	41.0
25	37.65619416167206	39.0	36.0	41.0	33.0	41.0
26	37.625042118053074	40.0	36.0	41.0	33.0	41.0
27	37.50001021043711	40.0	36.0	41.0	33.0	41.0
28	37.322088238597495	40.0	35.0	41.0	32.0	41.0
29	37.42253596626472	40.0	36.0	41.0	32.0	41.0
30	37.128263510960906	40.0	35.0	41.0	32.0	41.0
31	37.07303270403006	39.0	35.0	41.0	31.0	41.0
32	36.68648597596463	40.0	35.0	41.0	30.0	41.0
33	36.48693829832855	40.0	35.0	41.0	30.0	41.0
34	36.33560940993884	40.0	35.0	41.0	30.0	41.0
35	36.02988084419894	40.0	35.0	41.0	26.0	41.0
36	35.9701931814701	39.0	35.0	41.0	27.0	41.0
37	35.88566607786479	40.0	35.0	41.0	26.0	41.0
38	35.702618466596554	39.0	35.0	41.0	24.0	41.0
39	35.65527266972299	39.0	35.0	41.0	24.0	41.0
40	35.48722163795832	39.0	35.0	41.0	24.0	41.0
41	35.332957248899824	39.0	35.0	41.0	24.0	41.0
42	35.37071034010966	39.0	35.0	41.0	23.0	41.0
43	35.41905165460133	39.0	35.0	41.0	24.0	41.0
44	35.666156995680986	39.0	35.0	41.0	25.0	41.0
45	35.69484321873819	39.0	35.0	41.0	26.0	41.0
46	35.57721132541684	39.0	35.0	41.0	25.0	41.0
47	35.40359560542787	39.0	35.0	41.0	24.0	41.0
48	35.3707613922952	39.0	35.0	41.0	24.0	41.0
49	35.33761320822144	39.0	35.0	41.0	24.0	41.0
50	35.24691900060242	39.0	35.0	41.0	24.0	41.0
51	34.41849263316963	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	2.0
13	6.0
14	9.0
15	13.0
16	20.0
17	36.0
18	99.0
19	335.0
20	919.0
21	1964.0
22	2991.0
23	3877.0
24	4334.0
25	5075.0
26	5870.0
27	5927.0
28	5346.0
29	4989.0
30	5293.0
31	6717.0
32	8487.0
33	11788.0
34	19564.0
35	25733.0
36	27599.0
37	43114.0
38	81848.0
39	119762.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.78572376683446	15.600782119482535	19.822542603048838	15.790951510634171
2	27.505641266502618	38.23145018838257	20.76241333891504	13.500495206199778
3	23.679024699047364	37.82022483382514	26.623459500301205	11.87729096682629
4	21.960608133634203	20.11660319178264	43.757083490744236	14.16570518383892
5	35.214010761800715	22.552047703162174	25.22794800845424	17.005993526582873
6	19.72758553793688	43.8055830670111	25.104912241293047	11.361919153758974
7	66.00588121177468	3.5562952449994385	26.55019961404548	3.8876239291804082
8	62.91722398635886	17.11013998509276	13.966346399289353	6.006289629259029
9	58.40267921869735	5.065397849681945	14.268064815854768	22.263858115765935
10	41.035236218462515	17.671203504222017	23.102645524254896	18.19091475306058
11	30.643819111896182	18.6950550853082	28.63006565311061	22.031060149685008
12	21.29871654805542	15.450178172127549	34.08575746127692	29.16534781854011
13	24.28705622887716	17.624490754449198	40.76797802713934	17.3204749895343
14	18.89237178243601	28.635936654448173	30.586130142231386	21.88556142088443
15	14.5687621887093	19.981825421946315	41.514360979793544	23.93505140955084
16	16.386730515933387	21.480207067664566	29.693482677993444	32.439579738408604
17	17.73272138780261	26.334759391049527	33.33095089800794	22.601568323139915
18	17.462910587202238	21.66986593696076	32.539131500219526	28.328091975617475
19	18.0630290282727	23.24533638285055	28.900642236494146	29.790992352382606
20	23.68846935337302	22.777187841411493	35.224221198909525	18.310121606305966
21	21.318882161345325	31.208201023085795	29.111998284646567	18.36091853092231
22	18.738194182092936	19.263265910413622	35.619620375948294	26.378919531545147
23	22.99390436904604	25.88932907217758	30.21472549239833	20.902041066378054
24	22.108404210784265	24.41009199603835	27.418597290149993	26.062906503027396
25	17.37586661084961	34.02832375253985	25.607265747046632	22.98854388956391
26	18.105402342274274	20.70676645667201	33.26356201308978	27.924269187963937
27	24.997957912578237	25.100317544594088	25.919194600720857	23.982529942106822
28	17.15659747393786	25.728004165858344	32.40001429461195	24.71538406559185
29	20.33331971941719	19.131296010782222	30.588682751508596	29.946701518291995
30	19.070288649057066	33.00268534495962	28.041944475643003	19.885081530340315
31	26.53360765374366	20.992913956646483	23.257333646453404	29.216144743156452
32	25.54293999326111	26.6699169891463	24.03051899651824	23.756624021074344
33	24.077487007218778	25.562850345623296	23.531739143752745	26.82792350340518
34	27.489559828056237	20.573264991474286	24.61991647862445	27.317258701845027
35	22.53341365543859	24.6280848283115	27.59804572233737	25.240455793912535
36	23.251207384188117	30.079692461634284	22.488232471232095	24.180867682945507
37	20.18705520783345	26.256904808094834	28.837337526419503	24.71870245765221
38	22.961996753081	26.257925851805712	23.52178396757165	27.258293427541634
39	25.2562819714312	24.72304189342346	24.13645228152217	25.884223853623173
40	20.043088044599187	25.716262163183206	30.05467689071769	24.185972901499913
41	23.559307323946538	27.305261438242173	23.411766507724195	25.723664730087094
42	21.231327663137257	21.479186023953687	33.92009311918643	23.369393193722622
43	22.85708451178795	27.388221239751275	24.30339292825126	25.451301320209517
44	22.84968194488406	22.28300268534496	29.283788889002338	25.583526480768644
45	21.706112988697047	19.54252136533965	28.088912486343542	30.662453159619762
46	28.148388282502374	25.083725584292267	24.34627676410827	22.42160936909709
47	17.801386577359377	24.70211049735039	35.92516770642951	21.571335218860717
48	23.637672428756677	24.591071993792056	25.827811188596982	25.943444388854285
49	20.720040024913466	19.68699905042935	35.837102686366	23.75585823829118
50	23.017388374396308	21.262724757246858	29.788184482177684	25.93170238617915
51	20.856859882171555	20.18705520783345	27.115347308018258	31.84073760197674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	99.0
2	170.0
3	3066.0
4	5962.0
5	4055.0
6	2148.0
7	2161.0
8	2174.0
9	2125.5
10	2077.0
11	1992.5
12	1908.0
13	1779.0
14	1650.0
15	1507.0
16	1364.0
17	1316.0
18	1268.0
19	1202.5
20	1137.0
21	1113.0
22	1089.0
23	1241.0
24	1393.0
25	1445.0
26	1835.5
27	2174.0
28	2505.0
29	2836.0
30	3186.5
31	3537.0
32	4439.5
33	5342.0
34	5991.5
35	6641.0
36	7324.0
37	8007.0
38	8385.5
39	8764.0
40	9971.0
41	11178.0
42	12258.5
43	13339.0
44	16122.0
45	18905.0
46	46832.5
47	74760.0
48	56017.5
49	37275.0
50	37313.0
51	37351.0
52	32497.5
53	27644.0
54	25318.0
55	22992.0
56	21292.0
57	19592.0
58	17453.0
59	15314.0
60	14103.0
61	12892.0
62	11898.0
63	10904.0
64	10038.5
65	9173.0
66	7975.5
67	6778.0
68	5798.0
69	4818.0
70	3918.0
71	3018.0
72	2576.5
73	2135.0
74	1776.0
75	997.0
76	577.0
77	423.0
78	269.0
79	203.5
80	138.0
81	106.5
82	75.0
83	45.5
84	16.0
85	21.0
86	26.0
87	15.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.47371191380788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93890933348573	25.48948659583748
2	9.137035791392009	6.299750792371994
3	3.461161919729782	3.5795729672342023
4	2.0384880199802224	2.810969949621873
5	1.5851469728941496	2.7322950042298775
6	1.279941197851159	2.6474594472801014
7	1.125141304150588	2.7151458027129425
8	0.9519241696916009	2.625308767179121
9	0.8605768383923043	2.6700550205788707
>10	5.531170094753649	29.521348765161793
>50	0.05637976379992259	1.3597665961884957
>100	0.02151333092365467	1.2416149754551715
>500	0.005192872981571817	1.279208672961229
>1k	0.005192872981571817	5.307872052641007
>5k	0.0	0.0
>10k+	0.002225516992102207	9.720144590545862
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	13933	3.5565505059271585	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	12585	3.212458775360173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	11490	2.9329480595064275	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	3792	0.9679494379154372	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	3555	0.9074525980457224	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3379	0.8625266747669468	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCT	2623	0.6695494134103881	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCT	2580	0.6585731935184146	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	2479	0.6327918398186626	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTC	2347	0.599097397359581	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTT	998	0.2547504058648751	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	843	0.21518496206822615	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	795	0.20293243753765097	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATG	771	0.1968061752723634	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGCAGTA	544	0.13886194467985175	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGT	543	0.13860668375213142	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTAT	508	0.12967255128192037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.105218554406314E-4	0.0	0.0	0.21365339650190424	0.0
2	5.105218554406314E-4	0.0	0.0	1.6112069757706327	0.0
3	5.105218554406314E-4	0.0	0.0	1.9527460970604151	0.0
4	5.105218554406314E-4	0.0	0.0	2.9906370291712188	0.0
5	5.105218554406314E-4	0.0	0.0	7.207037033255394	0.0
6	5.105218554406314E-4	0.0	0.0	8.179325906942076	0.0
7	5.105218554406314E-4	0.0	0.0	9.498259120472948	0.0
8	5.105218554406314E-4	0.0	0.0	11.161539325498525	0.0
9	5.105218554406314E-4	0.0	0.0	11.527072973994017	0.0
10	5.105218554406314E-4	0.0	0.0	15.301105790338884	0.0
11	5.105218554406314E-4	0.0	0.0	16.54320546462594	0.0
12	5.105218554406314E-4	0.0	0.0	21.200951612738542	0.0
13	5.105218554406314E-4	0.0	0.0	21.794943791543716	0.0
14	5.105218554406314E-4	0.0	0.0	22.083899161723114	0.0
15	5.105218554406314E-4	0.0	0.0	23.007943720070656	0.0
16	5.105218554406314E-4	0.0	0.0	23.761473978701027	0.0
17	5.105218554406314E-4	0.0	0.0	24.5451250268024	0.0
18	5.105218554406314E-4	0.0	0.0	25.25960036349156	0.0
19	5.105218554406314E-4	0.0	0.0	26.74547422375152	0.0
20	5.105218554406314E-4	0.0	0.0	27.367034582750488	0.0
21	5.105218554406314E-4	0.0	0.0	27.9929343775207	0.0
22	5.105218554406314E-4	0.0	0.0	28.673460010823064	0.0
23	5.105218554406314E-4	0.0	0.0	29.289659890339905	0.0
24	5.105218554406314E-4	0.0	0.0	29.798394919286494	0.0
25	5.105218554406314E-4	0.0	0.0	30.21498075332605	0.0
26	5.105218554406314E-4	0.0	0.0	30.650455896016908	0.0
27	5.105218554406314E-4	0.0	0.0	31.091802040045334	0.0
28	5.105218554406314E-4	0.0	0.0	31.49230643563851	0.0
29	5.105218554406314E-4	0.0	0.0	31.894597657725726	0.0
30	5.105218554406314E-4	0.0	0.0	32.3290517567057	0.0
31	5.105218554406314E-4	0.0	0.0	32.751763853010544	0.0
32	5.105218554406314E-4	0.0	0.0	33.1808574725084	0.0
33	5.105218554406314E-4	0.0	0.0	33.616332615199255	0.0
34	5.105218554406314E-4	0.0	0.0	34.04823410490203	0.0
35	5.105218554406314E-4	0.0	0.0	34.46533046079703	0.0
36	5.105218554406314E-4	0.0	0.0	34.86660063917336	0.0
37	5.105218554406314E-4	0.0	0.0	35.289823257333644	0.0
38	5.105218554406314E-4	0.0	0.0	35.69645391519211	0.0
39	5.105218554406314E-4	0.0	0.0	36.0946609624358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGA	30	2.1618253E-6	45.000004	41
CGATCGA	115	0.0	45.000004	41
GCATAAG	20	7.0275884E-4	45.0	1
TCGCAGG	45	3.8380676E-10	45.0	2
CGAGTGG	20	7.0275884E-4	45.0	29
ACCGCAG	25	3.8860824E-5	45.0	23
CTCCATA	20	7.0275884E-4	45.0	11
CACGATC	20	7.0275884E-4	45.0	39
TGACGCG	20	7.0275884E-4	45.0	1
TAGCACG	20	7.0275884E-4	45.0	1
ACGATCG	50	2.1827873E-11	45.0	40
TGCGATG	20	7.0275884E-4	45.0	1
TACGACC	20	7.0275884E-4	45.0	6
AACGCGA	20	7.0275884E-4	45.0	25
CGTAAGC	45	3.8380676E-10	45.0	43
CACCGTA	25	3.8860824E-5	45.0	40
TAGCTAG	20	7.0275884E-4	45.0	1
GCACTAC	20	7.0275884E-4	45.0	9
AAATGCG	20	7.0275884E-4	45.0	1
CCGACTT	55	1.8189894E-12	44.999996	44
>>END_MODULE