SRR3551950_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740472
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	28378	3.8324204021218895	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	24288	3.28007000939941	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	24119	3.257246729113322	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7988	1.0787713782560313	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	7752	1.0468998152529738	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6267	0.8463520565261076	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	5800	0.7832841754988711	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	4823	0.651341306626044	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	4277	0.5776045549325295	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	4245	0.5732829870677082	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1992	0.2690175995851295	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1749	0.23620069361164228	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	1442	0.19474065190851242	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1404	0.18960879006903705	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	1353	0.182721291284478	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	1049	0.1416663965686751	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	1042	0.14072105359824544	No Hit
TGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCTT	796	0.10749900063743126	TruSeq Adapter, Index 15 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	7.0316583E-4	45.000004	13
AATTTCG	20	7.0316583E-4	45.000004	27
ATTAGCG	35	1.2111377E-7	45.000004	1
GCGAATG	20	7.0316583E-4	45.000004	1
TTCGTAG	20	7.0316583E-4	45.000004	1
TTATCCG	20	7.0316583E-4	45.000004	1
ACGGCTA	40	6.8102963E-9	45.000004	30
CGGCTAT	20	7.0316583E-4	45.000004	31
CGCGTAA	30	2.1644519E-6	45.000004	41
CAATCGT	20	7.0316583E-4	45.000004	10
CAATACG	20	7.0316583E-4	45.000004	17
CGATTTA	45	3.8380676E-10	45.0	10
CTCGAAT	565	0.0	44.20354	43
CGTTTTT	3860	0.0	43.834198	1
TACGGCT	3060	0.0	43.676468	7
TCGAATG	600	0.0	43.5	44
TGATACC	2645	0.0	43.29868	4
GATACCT	2640	0.0	43.210228	5
ACGGCTG	3105	0.0	43.188404	8
CGATGAA	2910	0.0	42.912373	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content