##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740472
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.035112738901674	33.0	31.0	34.0	30.0	34.0
2	32.14424043042816	33.0	31.0	34.0	30.0	34.0
3	32.14851473114446	33.0	31.0	34.0	30.0	34.0
4	35.78337330783608	37.0	35.0	37.0	35.0	37.0
5	35.78939379206776	37.0	35.0	37.0	35.0	37.0
6	35.669563467626055	37.0	35.0	37.0	35.0	37.0
7	35.84199402543243	37.0	35.0	37.0	35.0	37.0
8	35.04905924869543	37.0	35.0	37.0	32.0	37.0
9	36.6946164068324	39.0	37.0	39.0	32.0	39.0
10	36.6441337417215	38.0	35.0	39.0	32.0	39.0
11	37.05137398848302	39.0	37.0	39.0	34.0	39.0
12	37.08730917576897	39.0	37.0	39.0	34.0	39.0
13	37.14507638371201	39.0	37.0	39.0	33.0	39.0
14	38.29547909981741	40.0	38.0	41.0	34.0	41.0
15	38.17114354087663	40.0	38.0	41.0	33.0	41.0
16	38.44704863924632	40.0	38.0	41.0	34.0	41.0
17	38.1669948357264	40.0	37.0	41.0	33.0	41.0
18	38.12492437256237	40.0	37.0	41.0	34.0	41.0
19	37.99442247647446	40.0	37.0	41.0	33.0	41.0
20	37.68121144351171	40.0	36.0	41.0	32.0	41.0
21	37.958874879806395	40.0	36.0	41.0	33.0	41.0
22	37.98858565887704	40.0	37.0	41.0	33.0	41.0
23	37.880270962305126	40.0	36.0	41.0	33.0	41.0
24	37.817624434144705	40.0	36.0	41.0	33.0	41.0
25	37.811432437688396	40.0	36.0	41.0	33.0	41.0
26	37.780661524000905	40.0	36.0	41.0	33.0	41.0
27	37.67213074903575	40.0	36.0	41.0	33.0	41.0
28	37.509344040017716	40.0	36.0	41.0	33.0	41.0
29	37.64646198640867	40.0	36.0	41.0	33.0	41.0
30	37.293961149104895	40.0	36.0	41.0	32.0	41.0
31	37.21357593534935	40.0	36.0	41.0	31.0	41.0
32	36.95490578982055	40.0	36.0	41.0	31.0	41.0
33	36.70958658801413	40.0	35.0	41.0	30.0	41.0
34	36.451529564926155	40.0	35.0	41.0	30.0	41.0
35	36.373671117881564	40.0	35.0	41.0	29.0	41.0
36	36.16616563489234	40.0	35.0	41.0	28.0	41.0
37	36.10505191283398	40.0	35.0	41.0	28.0	41.0
38	35.90392479391523	39.0	35.0	41.0	26.0	41.0
39	35.84070160654285	39.0	35.0	41.0	26.0	41.0
40	35.69590612474206	39.0	35.0	41.0	25.0	41.0
41	35.65815722944284	39.0	35.0	41.0	25.0	41.0
42	35.69735114899686	39.0	35.0	41.0	25.0	41.0
43	35.69488785531391	39.0	35.0	41.0	25.0	41.0
44	35.93519944035696	39.0	35.0	41.0	26.0	41.0
45	35.95422784386175	39.0	35.0	41.0	26.0	41.0
46	35.86192590671896	39.0	35.0	41.0	26.0	41.0
47	35.67453192018064	39.0	35.0	41.0	26.0	41.0
48	35.71106672500783	39.0	35.0	41.0	26.0	41.0
49	35.72897827331756	39.0	35.0	41.0	26.0	41.0
50	35.59486921855249	39.0	35.0	41.0	26.0	41.0
51	34.75815020689506	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	5.0
11	4.0
12	2.0
13	8.0
14	15.0
15	18.0
16	23.0
17	74.0
18	190.0
19	650.0
20	2161.0
21	4140.0
22	5510.0
23	6368.0
24	7146.0
25	8604.0
26	10006.0
27	10245.0
28	9651.0
29	8856.0
30	9847.0
31	12150.0
32	15696.0
33	21576.0
34	36090.0
35	45973.0
36	50476.0
37	78046.0
38	148473.0
39	248381.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.25578279799912	15.658931060188635	18.800305750926434	15.284980390885814
2	27.176989811903756	39.25536684709213	20.21494398167655	13.352699359327563
3	23.735266154560875	38.25505893538176	25.6576886094275	12.35198630062987
4	22.474178632007693	19.602631834829676	43.56113397940773	14.362055553754901
5	35.49708834365108	22.39449972450005	24.795400771399866	17.31301116044901
6	19.60870903963958	43.511976144945386	25.077112976587905	11.802201838827127
7	64.61662291079203	4.059167666029236	27.148494473795093	4.175714949383636
8	62.01301332123295	18.299949221577588	13.81821324776629	5.868824209423179
9	58.022450545057744	5.545381864540455	13.7343478213896	22.6978197690122
10	41.21168659989844	18.739263604835834	22.830302833868128	17.218746961397596
11	31.499232921703996	18.931438325824608	28.19471904406919	21.374609708402208
12	21.850117222528333	16.161988569452998	33.14723581715447	28.840658390864206
13	24.737599801207878	17.785277498676518	40.19895418057671	17.278168519538887
14	19.327672079430418	28.725866744454887	29.8874231571214	22.05903801899329
15	15.178021586231486	20.37079052280167	40.84759450728724	23.6035933836796
16	16.939465638133512	21.859840750224183	28.679004742920732	32.52168886872157
17	17.683180457870115	26.923502846832832	33.53563672900528	21.85767996629177
18	17.517610389049146	22.800457005801704	31.728005920547975	27.95392668460117
19	18.167331107725886	24.275192039671992	28.70614959107164	28.851327261530486
20	23.392376754286456	23.26502555127	35.87806156073424	17.46453613370931
21	21.192023466113504	31.653323825884033	29.43392862930671	17.720724078695753
22	18.636356270054776	19.858819779816116	35.647938071932494	25.85688587819661
23	22.76912563878175	26.902975399474933	29.911326829373696	20.416572132369623
24	22.300910770427514	24.082612171695892	27.6157639991789	26.000713058697695
25	16.96039823247874	34.75958577772016	25.698608455147525	22.58140753465357
26	17.65306453181214	20.925436748452338	35.36122905390076	26.060269665834763
27	26.489455374409836	24.49505180479478	26.466091898140647	22.54940092265474
28	16.205744444084313	25.846622154517657	35.20713814972072	22.74049525167731
29	23.270967707084132	18.221350706036148	34.408593437699196	24.099088149180524
30	22.33359262740522	29.6232673213842	29.782490087403712	18.26064996380687
31	24.078695750818397	20.469781436705237	28.656451560626195	26.79507125185017
32	30.435857128966386	27.113651832884972	25.080219103490748	17.370271934657893
33	24.474659406432654	23.63505979969533	24.554743460927625	27.335537332944394
34	27.69692844564008	21.38824965697555	29.1731490184639	21.741672878920472
35	27.122430017610387	24.663187804535486	24.881832128696292	23.332550049157835
36	21.039418100887	25.528851867457515	30.96605948638166	22.465670545273824
37	28.398913125681997	21.534642768396374	29.545749197808963	20.520694908112663
38	20.028036171523027	26.195318661610433	27.331891010058452	26.44475415680809
39	30.41924610248598	23.26191942436716	25.560048185481694	20.758786287665167
40	21.034016141055975	28.821886580451388	27.391312568199744	22.752784710292893
41	23.92811612052853	23.965524692358386	24.107461186918613	27.998898000194473
42	21.44226925528582	24.945035058719302	31.18294817359738	22.429747512397498
43	22.845833468382327	22.13074903575017	26.391814950464028	28.631602545403474
44	24.565682429585454	20.404957918732915	30.88503008891626	24.14432956276537
45	20.902748517162024	20.073007487116325	29.585318553571238	29.43892544215041
46	29.89404055791441	24.565007184606575	25.22971834181441	20.3112339156646
47	18.644459209801315	23.840604371265893	36.847173154420425	20.667763264512367
48	23.932302639397573	24.396060890891217	26.667720048833715	25.00391642087749
49	21.233888654803962	20.67856718417442	35.42011041605895	22.667433744962672
50	23.490827472206917	20.228313832258344	29.783030283386815	26.49782841214793
51	21.351921477111897	19.217877245864802	28.68184077183202	30.748360505191286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	254.0
2	424.0
3	5818.5
4	11213.0
5	7481.5
6	3750.0
7	3662.5
8	3575.0
9	3543.0
10	3511.0
11	3359.0
12	3207.0
13	3079.0
14	2951.0
15	2733.5
16	2516.0
17	2405.0
18	2294.0
19	2191.5
20	2089.0
21	2091.0
22	2093.0
23	2166.0
24	2239.0
25	2608.0
26	3565.5
27	4154.0
28	5190.5
29	6227.0
30	6849.5
31	7472.0
32	8876.0
33	10280.0
34	11766.0
35	13252.0
36	15031.5
37	16811.0
38	18299.5
39	19788.0
40	21554.0
41	23320.0
42	25054.5
43	26789.0
44	32101.0
45	37413.0
46	92924.0
47	148435.0
48	109517.5
49	70600.0
50	69304.5
51	68009.0
52	58383.0
53	48757.0
54	45789.0
55	42821.0
56	39516.0
57	36211.0
58	32709.0
59	29207.0
60	26614.0
61	24021.0
62	21831.5
63	19642.0
64	17427.5
65	15213.0
66	12886.0
67	10559.0
68	9063.0
69	7567.0
70	6197.5
71	4828.0
72	3903.5
73	2979.0
74	2301.0
75	1281.0
76	939.0
77	655.0
78	371.0
79	271.0
80	171.0
81	115.0
82	59.0
83	40.0
84	21.0
85	14.5
86	8.0
87	4.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	740472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.892445125267898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87003113685208	23.24003469308618
2	9.341451394035525	5.958434519492706
3	3.5317733912633402	3.3791066722724215
4	2.0593708701916795	2.627134898806533
5	1.474297163554762	2.350947069350418
6	1.2916545633245644	2.4716413368978327
7	1.1105912737099268	2.4793629877376655
8	1.012852318936706	2.584186960135139
9	0.9126626603802688	2.619633943265286
>10	6.297653137112409	31.547361194243617
>50	0.05970608164912243	1.306534656019157
>100	0.02857362474504045	1.609162137944433
>500	0.0021323600556000335	0.42123025451896784
>1k	0.004264720111200067	3.179424743182239
>5k	0.0017058880444800269	3.7820954754307197
>10k+	0.0012794160333600203	10.443708457616708
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	28378	3.8324204021218895	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	24288	3.28007000939941	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	24119	3.257246729113322	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	7988	1.0787713782560313	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	7752	1.0468998152529738	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6267	0.8463520565261076	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	5800	0.7832841754988711	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	4823	0.651341306626044	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	4277	0.5776045549325295	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	4245	0.5732829870677082	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1992	0.2690175995851295	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1749	0.23620069361164228	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	1442	0.19474065190851242	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1404	0.18960879006903705	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	1353	0.182721291284478	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	1049	0.1416663965686751	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	1042	0.14072105359824544	No Hit
TGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCTT	796	0.10749900063743126	TruSeq Adapter, Index 15 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19906221977333377	0.0
2	0.0	0.0	0.0	1.6764982335591352	0.0
3	0.0	0.0	0.0	1.9919726876910944	0.0
4	0.0	0.0	0.0	2.9474443328039412	0.0
5	0.0	0.0	0.0	7.107628647673376	0.0
6	0.0	0.0	0.0	8.017858879201373	0.0
7	0.0	0.0	0.0	9.421288043302111	0.0
8	0.0	0.0	0.0	10.88751499043853	0.0
9	0.0	0.0	0.0	11.241208310375004	0.0
10	0.0	0.0	0.0	15.305507838243715	0.0
11	0.0	0.0	0.0	16.397243920094212	0.0
12	0.0	0.0	0.0	21.213901403429166	0.0
13	0.0	0.0	0.0	21.73086895925842	0.0
14	0.0	0.0	0.0	22.011770870471807	0.0
15	0.0	0.0	0.0	22.850019987251375	0.0
16	0.0	0.0	0.0	23.517026977387395	0.0
17	0.0	0.0	0.0	24.210638619691224	0.0
18	0.0	0.0	0.0	24.815388022774663	0.0
19	0.0	0.0	0.0	26.452046802579975	0.0
20	0.0	0.0	0.0	27.07003100724943	0.0
21	0.0	0.0	0.0	27.629944143735347	0.0
22	0.0	0.0	0.0	28.305594269601013	0.0
23	0.0	0.0	0.0	28.866857896044685	0.0
24	0.0	0.0	0.0	29.322648256787563	0.0
25	0.0	0.0	0.0	29.73859916377662	0.0
26	0.0	0.0	0.0	30.15036355189663	0.0
27	0.0	0.0	0.0	30.60385807971132	0.0
28	0.0	0.0	0.0	30.996445510431183	0.0
29	0.0	0.0	0.0	31.394704998973626	0.0
30	0.0	0.0	0.0	31.818353698721896	0.0
31	0.0	0.0	0.0	32.24038181052086	0.0
32	0.0	0.0	0.0	32.64985036571268	0.0
33	0.0	0.0	0.0	33.0732289674694	0.0
34	1.350489957756674E-4	0.0	0.0	33.47864605278795	0.0
35	1.350489957756674E-4	0.0	0.0	33.90378029148975	0.0
36	1.350489957756674E-4	0.0	0.0	34.31378904266468	0.0
37	1.350489957756674E-4	0.0	0.0	34.711373286228245	0.0
38	2.700979915513348E-4	0.0	0.0	35.12894478116661	0.0
39	2.700979915513348E-4	0.0	0.0	35.52706922071327	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	20	7.0316583E-4	45.000004	13
AATTTCG	20	7.0316583E-4	45.000004	27
ATTAGCG	35	1.2111377E-7	45.000004	1
GCGAATG	20	7.0316583E-4	45.000004	1
TTCGTAG	20	7.0316583E-4	45.000004	1
TTATCCG	20	7.0316583E-4	45.000004	1
ACGGCTA	40	6.8102963E-9	45.000004	30
CGGCTAT	20	7.0316583E-4	45.000004	31
CGCGTAA	30	2.1644519E-6	45.000004	41
CAATCGT	20	7.0316583E-4	45.000004	10
CAATACG	20	7.0316583E-4	45.000004	17
CGATTTA	45	3.8380676E-10	45.0	10
CTCGAAT	565	0.0	44.20354	43
CGTTTTT	3860	0.0	43.834198	1
TACGGCT	3060	0.0	43.676468	7
TCGAATG	600	0.0	43.5	44
TGATACC	2645	0.0	43.29868	4
GATACCT	2640	0.0	43.210228	5
ACGGCTG	3105	0.0	43.188404	8
CGATGAA	2910	0.0	42.912373	19
>>END_MODULE