FastQCFastQC Report
Sat 18 Jun 2016
SRR3551948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493327
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC175973.5670052520944524No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC154923.1403105850683217No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG146742.974497645577882No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC49711.0076480711576703No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT44890.9099441141474114No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36170.733185088186943No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT34830.7060225773168709No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA29910.6062915672566066No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT29070.5892643216365616No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC26220.5314933097114085No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT10250.20777293762555057No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC10200.2067594111005479No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATG9700.19662414585052104No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT9220.18689429121049528No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGTGTAT8110.16439400235543564No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGT6760.13702878618036313No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT6720.13621796496036098No Hit
TGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCTT5420.10986627531029114Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG207.0293655E-445.0000046
ACCATTC207.0293655E-445.00000439
GCGAATA207.0293655E-445.00000433
CGCATGT302.162973E-645.00000439
AGACGTT302.162973E-645.00000411
CTAACGG207.0293655E-445.0000042
CCATCGA800.045.00000441
TACGAAT253.8875565E-545.00000412
AACGCGT253.8875565E-545.00000439
CTCATAG207.0293655E-445.00000424
TGTAGCG207.0293655E-445.0000041
CGTCGAT207.0293655E-445.00000441
CGGTCTA302.162973E-645.00000431
ACGCGTA253.8875565E-545.00000440
AACACCG351.2100463E-745.036
CCGATCG650.044.99999640
CGATCGA650.044.99999641
TACGGCT17950.043.621177
TGATACC16000.043.3125044
ACGGCTG18200.043.269238
Produced by FastQC (version 0.11.5)