##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493327
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994790473661485	33.0	31.0	34.0	30.0	34.0
2	32.091357253910694	33.0	31.0	34.0	30.0	34.0
3	32.10382768427433	33.0	31.0	34.0	30.0	34.0
4	35.764130080048325	37.0	35.0	37.0	35.0	37.0
5	35.75794148708666	37.0	35.0	37.0	35.0	37.0
6	35.648170483269716	37.0	35.0	37.0	35.0	37.0
7	35.85608531460877	37.0	35.0	37.0	35.0	37.0
8	35.09749922465221	37.0	35.0	37.0	32.0	37.0
9	36.734368887168145	39.0	37.0	39.0	32.0	39.0
10	36.566693085924754	38.0	35.0	39.0	32.0	39.0
11	37.010200130947624	39.0	37.0	39.0	34.0	39.0
12	37.05418920918579	39.0	37.0	39.0	33.0	39.0
13	37.10299456547077	39.0	37.0	39.0	33.0	39.0
14	38.26547705680005	40.0	38.0	41.0	33.0	41.0
15	38.1519195178857	40.0	37.0	41.0	33.0	41.0
16	38.407644422462184	40.0	38.0	41.0	34.0	41.0
17	38.14338359749213	40.0	37.0	41.0	33.0	41.0
18	38.06057239924026	40.0	37.0	41.0	34.0	41.0
19	37.997630374984546	40.0	37.0	41.0	34.0	41.0
20	37.719115312966856	40.0	36.0	41.0	32.0	41.0
21	37.918281383342084	40.0	36.0	41.0	33.0	41.0
22	37.92847948723666	40.0	37.0	41.0	33.0	41.0
23	37.81971390173252	40.0	36.0	41.0	33.0	41.0
24	37.73368982439639	40.0	36.0	41.0	33.0	41.0
25	37.74683323637263	39.0	36.0	41.0	33.0	41.0
26	37.72679987107943	40.0	36.0	41.0	33.0	41.0
27	37.61126798249437	40.0	36.0	41.0	33.0	41.0
28	37.455261925659855	40.0	36.0	41.0	32.0	41.0
29	37.529117603536804	40.0	36.0	41.0	33.0	41.0
30	37.250000506763264	40.0	36.0	41.0	32.0	41.0
31	37.2074344197662	39.0	35.0	41.0	31.0	41.0
32	36.825667356540386	39.0	35.0	41.0	30.0	41.0
33	36.74542848860897	40.0	35.0	41.0	30.0	41.0
34	36.52396483468369	40.0	35.0	41.0	30.0	41.0
35	36.33160966255648	40.0	35.0	41.0	30.0	41.0
36	36.21095541091406	40.0	35.0	41.0	29.0	41.0
37	36.23742872374713	40.0	35.0	41.0	29.0	41.0
38	35.99597427264269	39.0	35.0	41.0	27.0	41.0
39	35.72331942099257	39.0	35.0	41.0	26.0	41.0
40	35.79993189101752	39.0	35.0	41.0	26.0	41.0
41	35.61853699473169	39.0	35.0	41.0	25.0	41.0
42	35.70722664682857	39.0	35.0	41.0	26.0	41.0
43	35.68975142248448	39.0	35.0	41.0	26.0	41.0
44	35.98623428273741	39.0	35.0	41.0	28.0	41.0
45	35.95327237309127	39.0	35.0	41.0	28.0	41.0
46	35.837004664249065	39.0	35.0	41.0	28.0	41.0
47	35.636557496346235	39.0	35.0	41.0	27.0	41.0
48	35.69203591127184	39.0	35.0	41.0	27.0	41.0
49	35.64979212570972	39.0	35.0	41.0	27.0	41.0
50	35.461756603632075	39.0	35.0	41.0	26.0	41.0
51	34.83863441490127	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	2.0
12	2.0
13	5.0
14	11.0
15	20.0
16	29.0
17	53.0
18	101.0
19	343.0
20	1057.0
21	2293.0
22	3555.0
23	4336.0
24	4995.0
25	5722.0
26	6457.0
27	6553.0
28	6010.0
29	6003.0
30	6801.0
31	8341.0
32	10882.0
33	14689.0
34	24807.0
35	30894.0
36	34737.0
37	56536.0
38	106004.0
39	152033.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.451230117143396	16.542779941093837	20.672697825174783	14.333292116587984
2	26.984941022891512	38.233261102676316	21.605142228177254	13.176655646254916
3	23.552937503927414	38.00460951863571	26.259661441599587	12.182791535837284
4	23.04434989368107	20.161069635353428	42.95832176223884	13.836258708726664
5	34.64740425721682	23.52942368854735	24.783155999975676	17.040016054260157
6	19.58883255933691	44.33225831953248	24.77768295674066	11.301226164389949
7	67.90384471151995	3.2232170548135417	25.167687963561697	3.7052502701048184
8	64.55474766230107	17.00738050015507	12.795569672853906	5.642302164689952
9	60.22394882096459	4.66424906806236	13.224899508845045	21.886902602128
10	40.86397055097329	17.32846570327592	22.356570793814246	19.450992951936545
11	30.37316019597549	19.013960314355387	27.435554915907705	23.17732457376142
12	21.68541352895747	15.386346176065773	33.91948950695989	29.008750788016872
13	23.99584859535359	18.04178567157281	40.34545038078192	17.616915352291684
14	18.68801018391452	28.15475333804961	30.420795942650614	22.736440535385253
15	14.327616368047968	20.583710196279547	40.92843083796346	24.160242597709026
16	16.38771038276843	21.56561469370215	28.89118171111656	33.155493212412864
17	17.956852148777585	25.665532192643013	32.545958360276245	23.831657298303156
18	17.560765982806537	22.40684170945438	31.403105850683215	28.62928645705587
19	18.702807671179563	23.29894775676174	28.2254974894948	29.7727470825639
20	23.756453630147956	22.537789336484725	34.91173197493752	18.794025058429803
21	21.843523666857887	30.501269948735825	28.572326266350718	19.08288011805557
22	19.612346374716974	19.608494973921964	34.17449278065056	26.6046658707105
23	23.568343107107456	26.041550533419013	29.224834643147446	21.165271716326085
24	22.7165754154952	23.938482994038438	26.1510113981193	27.193930192347064
25	17.555698350181522	33.979287571935046	24.391123940104638	24.073890137778793
26	18.398749713678754	20.657900337909744	32.330685326365675	28.612664622045823
27	25.27268931155197	23.83307623543816	25.027821303111324	25.86641314989855
28	17.770363268177093	25.020321206826303	31.9617616712647	25.247553853731908
29	21.390477310181684	18.026785479002772	30.468431689325744	30.114305521489804
30	20.361139771388956	26.930818706456368	27.882317408128888	24.82572411402579
31	25.963914401603805	20.15438036028841	24.453354468739803	29.428350769367984
32	26.815682093216058	25.72370861517817	26.46824520044514	20.992364091160628
33	28.224686668274796	18.70645636666957	25.608774707242866	27.46008225781277
34	24.405110606149673	19.93140452478782	29.160171650852273	26.503313218210234
35	23.287190849071713	22.95090274807582	25.9622927591638	27.79961364368867
36	24.492882003214905	23.38428669016697	26.973792231116477	25.149039075501644
37	19.326126484056214	20.920403707885438	33.924962550194905	25.828507257863446
38	27.029333484686628	21.821834199222828	24.334771865314487	26.814060450776054
39	23.289420607426717	21.606358460007257	33.33954962935335	21.764671303212676
40	24.01875429481865	22.775359953945355	28.06272512957937	25.14316062165663
41	19.470452661216598	23.693209574987787	30.987357270127113	25.8489804936685
42	20.880673468105336	24.279838727659342	31.503647681963486	23.33584012227184
43	24.09436337358385	20.907227863060402	29.225848169672446	25.7725605936833
44	22.48731571553959	19.594508307876925	30.980262584452095	26.937913392131385
45	21.413788420256747	19.058555481455507	28.49469013453551	31.032965963752236
46	28.15272628499961	23.43090891031709	26.991630297956526	21.424734506726775
47	19.30950464904617	22.23758277977893	36.599659049676994	21.853253521497912
48	22.939348545690788	22.74292710514527	27.526366892547944	26.791357456615998
49	20.462897834499227	20.141407220768375	35.982826806560354	23.412868138172044
50	23.506720694387294	19.190111224400855	31.145061997417535	26.15810608379432
51	20.82594303575519	18.493413091114007	28.420297287600317	32.26034658553049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	155.0
2	265.0
3	3338.5
4	6412.0
5	4363.0
6	2314.0
7	2252.5
8	2191.0
9	2175.5
10	2160.0
11	2052.0
12	1944.0
13	1795.5
14	1647.0
15	1576.5
16	1506.0
17	1422.0
18	1338.0
19	1263.5
20	1189.0
21	1210.5
22	1232.0
23	1335.5
24	1439.0
25	1682.5
26	2295.5
27	2665.0
28	3009.5
29	3354.0
30	4017.5
31	4681.0
32	5140.5
33	5600.0
34	6725.5
35	7851.0
36	8699.0
37	9547.0
38	10543.5
39	11540.0
40	12581.0
41	13622.0
42	15115.0
43	16608.0
44	20088.5
45	23569.0
46	58892.5
47	94216.0
48	70000.5
49	45785.0
50	45383.0
51	44981.0
52	39407.0
53	33833.0
54	31970.5
55	30108.0
56	27665.0
57	25222.0
58	23062.5
59	20903.0
60	20505.5
61	20108.0
62	17996.5
63	15885.0
64	14259.0
65	12633.0
66	10856.5
67	9080.0
68	7755.5
69	6431.0
70	5298.0
71	4165.0
72	3395.0
73	2625.0
74	1977.0
75	1093.5
76	858.0
77	613.0
78	368.0
79	226.5
80	85.0
81	55.5
82	26.0
83	26.5
84	27.0
85	17.0
86	7.0
87	4.0
88	1.0
89	3.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.799902445157002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94596855105	22.467287151398306
2	9.269560491278254	5.710031176817036
3	3.3184608640626916	3.066248126436069
4	1.8830522894771193	2.319913072600993
5	1.352441316622762	2.0827530307390383
6	1.0720078685724646	1.981064266348356
7	1.0035334982634352	2.1636113692872656
8	0.9568130323484596	2.3575798443669926
9	0.8839517499125883	2.4503064897180185
>10	7.199724092737048	36.1006485393839
>50	0.0754418035085032	1.588812584927838
>100	0.027132578454812557	1.5115630698941431
>500	0.003970621237289642	0.9361662864109761
>1k	0.005955931855934463	5.528741676224196
>5k	0.0	0.0
>10k+	0.001985310618644821	9.735273315446866
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	17597	3.5670052520944524	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	15492	3.1403105850683217	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	14674	2.974497645577882	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	4971	1.0076480711576703	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT	4489	0.9099441141474114	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3617	0.733185088186943	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	3483	0.7060225773168709	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA	2991	0.6062915672566066	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	2907	0.5892643216365616	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	2622	0.5314933097114085	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	1025	0.20777293762555057	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1020	0.2067594111005479	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATG	970	0.19662414585052104	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	922	0.18689429121049528	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGTGTAT	811	0.16439400235543564	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGT	676	0.13702878618036313	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT	672	0.13621796496036098	No Hit
TGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCTT	542	0.10986627531029114	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19338086097051246	0.0
2	0.0	0.0	0.0	1.6224532612242994	0.0
3	0.0	0.0	0.0	1.9313761460451182	0.0
4	0.0	0.0	0.0	2.8832802583276407	0.0
5	0.0	0.0	0.0	7.062658236828716	0.0
6	0.0	0.0	0.0	7.980305152566148	0.0
7	0.0	0.0	0.0	9.347957845404773	0.0
8	0.0	0.0	0.0	10.835003962888713	0.0
9	0.0	0.0	0.0	11.174940759374614	0.0
10	0.0	0.0	0.0	14.971003006119673	0.0
11	0.0	0.0	0.0	16.122166433217725	0.0
12	0.0	0.0	0.0	20.688914249574825	0.0
13	0.0	0.0	0.0	21.208853356901205	0.0
14	0.0	0.0	0.0	21.486154214141937	0.0
15	0.0	0.0	0.0	22.361233015829256	0.0
16	0.0	0.0	0.0	23.086107186511178	0.0
17	0.0	0.0	0.0	23.844427732518188	0.0
18	0.0	0.0	0.0	24.50990924883495	0.0
19	0.0	0.0	0.0	25.98175246844385	0.0
20	0.0	0.0	0.0	26.60750374498051	0.0
21	0.0	0.0	0.0	27.191497728687057	0.0
22	0.0	0.0	0.0	27.862249582933835	0.0
23	0.0	0.0	0.0	28.42333786717532	0.0
24	0.0	0.0	0.0	28.862802968416485	0.0
25	0.0	0.0	0.0	29.280578602022594	0.0
26	2.0270530500053716E-4	0.0	0.0	29.68761085446367	0.0
27	2.0270530500053716E-4	0.0	0.0	30.16964406975495	0.0
28	2.0270530500053716E-4	0.0	0.0	30.548500284800955	0.0
29	4.054106100010743E-4	0.0	0.0	30.930397079421965	0.0
30	4.054106100010743E-4	0.0	0.0	31.327699477223018	0.0
31	4.054106100010743E-4	0.0	0.0	31.72783974929408	0.0
32	4.054106100010743E-4	0.0	0.0	32.13689905478516	0.0
33	6.081159150016115E-4	0.0	0.0	32.538458263991224	0.0
34	6.081159150016115E-4	0.0	0.0	32.91954423739224	0.0
35	6.081159150016115E-4	0.0	0.0	33.335292817948336	0.0
36	6.081159150016115E-4	0.0	0.0	33.76867676003949	0.0
37	6.081159150016115E-4	0.0	0.0	34.16678997906054	0.0
38	6.081159150016115E-4	0.0	0.0	34.56247073442159	0.0
39	6.081159150016115E-4	0.0	0.0	34.96261100649265	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTG	20	7.0293655E-4	45.000004	6
ACCATTC	20	7.0293655E-4	45.000004	39
GCGAATA	20	7.0293655E-4	45.000004	33
CGCATGT	30	2.162973E-6	45.000004	39
AGACGTT	30	2.162973E-6	45.000004	11
CTAACGG	20	7.0293655E-4	45.000004	2
CCATCGA	80	0.0	45.000004	41
TACGAAT	25	3.8875565E-5	45.000004	12
AACGCGT	25	3.8875565E-5	45.000004	39
CTCATAG	20	7.0293655E-4	45.000004	24
TGTAGCG	20	7.0293655E-4	45.000004	1
CGTCGAT	20	7.0293655E-4	45.000004	41
CGGTCTA	30	2.162973E-6	45.000004	31
ACGCGTA	25	3.8875565E-5	45.000004	40
AACACCG	35	1.2100463E-7	45.0	36
CCGATCG	65	0.0	44.999996	40
CGATCGA	65	0.0	44.999996	41
TACGGCT	1795	0.0	43.62117	7
TGATACC	1600	0.0	43.312504	4
ACGGCTG	1820	0.0	43.26923	8
>>END_MODULE