SRR3551945_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357750
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	12000	3.3542976939203357	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	11344	3.170929419986024	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	11085	3.09853249475891	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3998	1.117540181691125	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	3471	0.9702306079664571	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2825	0.7896575821104124	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	2814	0.7865828092243187	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	2435	0.6806429070580015	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	2248	0.6283717679944095	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	2084	0.5825296995108316	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1315	0.36757512229210343	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1025	0.286512928022362	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	958	0.26778476589797345	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG	753	0.21048218029350105	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG	605	0.16911250873515024	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT	535	0.14954577218728163	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	524	0.14647099930118798	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	480	0.13417190775681342	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCTT	431	0.12047519217330538	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	372	0.10398322851153041	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.026766E-4	45.0	1
TACCAGA	40	6.7921064E-9	45.0	43
TAGACGG	55	1.8189894E-12	45.0	2
ACCGCGT	40	6.7921064E-9	45.0	39
TTAGACG	20	7.026766E-4	45.0	1
GTCTCGT	20	7.026766E-4	45.0	27
GTAGGTA	25	3.885402E-5	45.0	9
CCAGACA	40	6.7921064E-9	45.0	45
CCCCGTA	40	6.7921064E-9	45.0	40
TGTTCTA	20	7.026766E-4	45.0	11
TCGTACG	20	7.026766E-4	45.0	30
CCGAATG	20	7.026766E-4	45.0	44
CCGCGTA	40	6.7921064E-9	45.0	40
ATACGGT	20	7.026766E-4	45.0	28
CGCGTAA	40	6.7921064E-9	45.0	41
ATACGCC	25	3.885402E-5	45.0	28
GACGAAT	25	3.885402E-5	45.0	20
ACCCCGT	40	6.7921064E-9	45.0	39
GATTACG	25	3.885402E-5	45.0	1
TTACACG	25	3.885402E-5	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content