##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357750
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.032293501048215	33.0	31.0	34.0	30.0	34.0
2	32.13209503843466	33.0	31.0	34.0	30.0	34.0
3	32.14905101327743	33.0	31.0	34.0	30.0	34.0
4	35.770722571628234	37.0	35.0	37.0	35.0	37.0
5	35.79540740740741	37.0	35.0	37.0	35.0	37.0
6	35.691603074772885	37.0	35.0	37.0	35.0	37.0
7	35.88914884696017	37.0	35.0	37.0	35.0	37.0
8	35.215041229909154	37.0	35.0	37.0	32.0	37.0
9	36.81569252271139	39.0	37.0	39.0	32.0	39.0
10	36.675253668763105	38.0	35.0	39.0	32.0	39.0
11	37.11276030747729	39.0	37.0	39.0	34.0	39.0
12	37.14524109014675	39.0	37.0	39.0	34.0	39.0
13	37.241705101327746	39.0	37.0	39.0	34.0	39.0
14	38.39275471698113	40.0	38.0	41.0	34.0	41.0
15	38.29989378057303	40.0	38.0	41.0	33.0	41.0
16	38.505243885394826	40.0	38.0	41.0	34.0	41.0
17	38.2739426974144	40.0	38.0	41.0	33.0	41.0
18	38.18691544374563	40.0	38.0	41.0	34.0	41.0
19	38.11830328441649	40.0	37.0	41.0	34.0	41.0
20	37.867111111111114	40.0	36.0	41.0	33.0	41.0
21	38.03160027952481	40.0	37.0	41.0	34.0	41.0
22	38.04178336827393	40.0	37.0	41.0	34.0	41.0
23	37.958526904262754	40.0	36.0	41.0	33.0	41.0
24	37.89838714185884	40.0	36.0	41.0	33.0	41.0
25	37.85897693920335	40.0	36.0	41.0	33.0	41.0
26	37.88233962264151	40.0	36.0	41.0	33.0	41.0
27	37.76904821802935	40.0	36.0	41.0	33.0	41.0
28	37.596497554157935	40.0	36.0	41.0	33.0	41.0
29	37.770521313766594	40.0	36.0	41.0	33.0	41.0
30	37.41392592592592	40.0	36.0	41.0	33.0	41.0
31	37.35933752620545	40.0	36.0	41.0	32.0	41.0
32	37.087619846261354	40.0	36.0	41.0	32.0	41.0
33	36.928447239692524	40.0	36.0	41.0	31.0	41.0
34	36.63055485674354	40.0	35.0	41.0	30.0	41.0
35	36.592679245283016	40.0	36.0	41.0	30.0	41.0
36	36.37824458420685	40.0	35.0	41.0	30.0	41.0
37	36.200637316561846	40.0	35.0	41.0	30.0	41.0
38	36.20636757512229	39.0	35.0	41.0	29.0	41.0
39	36.05155276030748	39.0	35.0	41.0	29.0	41.0
40	36.09559189378057	39.0	35.0	41.0	28.0	41.0
41	35.78650454227813	39.0	35.0	41.0	26.0	41.0
42	35.86770649895178	39.0	35.0	41.0	28.0	41.0
43	35.77398183088749	39.0	35.0	41.0	28.0	41.0
44	35.94908176100629	39.0	35.0	41.0	29.0	41.0
45	36.03057442348008	39.0	35.0	41.0	29.0	41.0
46	35.9622641509434	39.0	35.0	41.0	29.0	41.0
47	35.758719776380154	39.0	35.0	41.0	28.0	41.0
48	35.768620545073375	39.0	35.0	41.0	28.0	41.0
49	35.832696016771486	39.0	35.0	41.0	28.0	41.0
50	35.623474493361286	39.0	35.0	41.0	28.0	41.0
51	34.774423480083854	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	0.0
12	5.0
13	6.0
14	7.0
15	9.0
16	18.0
17	36.0
18	80.0
19	262.0
20	870.0
21	1695.0
22	2306.0
23	2639.0
24	3024.0
25	3856.0
26	4555.0
27	4531.0
28	4256.0
29	4231.0
30	4511.0
31	5668.0
32	7317.0
33	10201.0
34	17505.0
35	22962.0
36	25622.0
37	40349.0
38	76569.0
39	114624.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.07085953878406	16.295457721872815	21.745911949685535	14.887770789657582
2	27.365478686233402	37.76995108315863	21.293361285814115	13.57120894479385
3	23.859119496855346	37.1141858839972	27.000698812019568	12.025995807127883
4	22.321733053808526	19.576799440950385	44.03941299790357	14.062054507337526
5	33.87728860936408	23.801257861635218	25.525925925925925	16.795527603074774
6	19.277987421383646	43.85017470300489	25.38001397624039	11.491823899371068
7	66.83913347309573	3.302865129280223	25.763801537386442	4.094199860237596
8	63.38784067085954	15.958071278825997	13.930118798043326	6.723969252271139
9	59.18099231306778	4.678127183787561	14.433822501747029	21.707058001397623
10	40.06261355695318	16.408385744234803	24.25269042627533	19.276310272536687
11	29.063032844164923	18.770370370370372	28.581970649895176	23.58462613556953
12	20.566317260656884	15.298392732354996	35.129559748427674	29.005730258560448
13	23.00824598183089	17.60251572327044	41.461635220125785	17.927603074772886
14	18.43466107617051	27.728860936408108	31.926764500349407	21.909713487071976
15	14.399720475192174	19.872816212438853	42.06708595387841	23.660377358490567
16	15.578197064989519	21.2324248777079	30.204053109713485	32.9853249475891
17	17.23969252271139	25.488749126484976	32.99566736547869	24.275890985324946
18	17.244444444444447	21.338085255066385	33.52229210342418	27.89517819706499
19	18.309154437456325	22.973584905660378	28.60405310971349	30.11320754716981
20	22.62445842068484	22.336827393431165	35.59720475192174	19.441509433962263
21	21.944654088050314	29.25590496156534	29.392033542976943	19.407407407407405
22	18.683717679944095	19.442348008385743	34.47071977638016	27.403214535290005
23	21.946331236897272	25.699790356394132	30.271698113207545	22.082180293501047
24	22.413976240391335	23.78197064989518	27.038155136268344	26.765897973445142
25	16.822082459818308	32.625576519916144	26.072117400419287	24.48022361984626
26	17.44849755415793	20.147030048916843	34.70328441649196	27.70118798043326
27	24.75583508036338	22.771488469601678	28.10146750524109	24.37120894479385
28	16.968832983927324	24.22753319357093	34.055625436757516	24.748008385744235
29	20.574143955276032	17.75653389238295	36.426834381551366	25.24248777078966
30	19.38280922431866	26.25101327742837	33.243605870020964	21.122571628232006
31	25.47113906359189	21.45296995108316	26.146750524109013	26.929140461215933
32	26.968553459119498	23.60251572327044	30.262753319357095	19.16617749825297
33	24.36869322152341	18.53724668064291	26.421523410202656	30.672536687631023
34	25.30649895178197	22.540041928721173	29.290006988120194	22.86345213137666
35	26.572746331236896	19.172886093640813	30.38211041229909	23.872257162823203
36	28.835779175401814	22.634800838574424	27.681621243885395	20.847798742138366
37	21.377218728162127	20.97610062893082	37.10775681341719	20.538923829489868
38	26.512928022361987	20.711670160726765	24.013696715583507	28.761705101327745
39	24.208525506638715	24.691264849755417	29.485953878406708	21.61425576519916
40	23.737246680642908	19.676589797344516	29.00125786163522	27.584905660377355
41	24.618308874912646	26.682320055904963	23.887351502445842	24.81201956673655
42	27.035639412997902	19.016072676450037	31.427253668763104	22.52103424178896
43	28.905939902166317	19.75206149545772	27.26932215234102	24.07267645003494
44	22.777358490566037	19.906079664570232	32.08106219426974	25.23549965059399
45	22.495877009084555	18.15653389238295	29.36408106219427	29.983508036338225
46	29.712648497554156	22.17833682739343	27.24472396925227	20.86429070580014
47	19.02278127183788	20.927463312368975	37.20587002096436	22.843885394828792
48	23.766876310272536	23.240251572327043	26.754437456324247	26.23843466107617
49	22.649615653389237	18.099510831586304	36.06624737945492	23.18462613556953
50	25.00600978336827	17.91530398322851	30.817889587700908	26.260796645702307
51	21.853249475890983	17.967295597484277	28.77819706498952	31.40125786163522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	105.0
2	183.0
3	2542.5
4	4902.0
5	3315.5
6	1729.0
7	1740.0
8	1751.0
9	1696.5
10	1642.0
11	1542.0
12	1442.0
13	1381.5
14	1321.0
15	1227.5
16	1134.0
17	1029.0
18	924.0
19	945.0
20	966.0
21	982.0
22	998.0
23	988.0
24	978.0
25	1163.0
26	1536.5
27	1725.0
28	2018.0
29	2311.0
30	2755.0
31	3199.0
32	3654.0
33	4109.0
34	4822.0
35	5535.0
36	6107.5
37	6680.0
38	7289.5
39	7899.0
40	9080.0
41	10261.0
42	11571.0
43	12881.0
44	15348.0
45	17815.0
46	44804.5
47	71794.0
48	54644.5
49	37495.0
50	35986.0
51	34477.0
52	29033.0
53	23589.0
54	21978.0
55	20367.0
56	18400.5
57	16434.0
58	15104.5
59	13775.0
60	12750.5
61	11726.0
62	10837.5
63	9949.0
64	9163.5
65	8378.0
66	7172.5
67	5967.0
68	5345.5
69	4724.0
70	4094.0
71	3464.0
72	2719.5
73	1975.0
74	1465.5
75	751.0
76	546.0
77	365.5
78	185.0
79	168.0
80	151.0
81	89.0
82	27.0
83	17.5
84	8.0
85	4.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.53500276076741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9921755242312	24.47792807719513
2	9.508847426118528	6.377584493732018
3	3.5212911604460437	3.54259526361072
4	2.016260593130365	2.7046121822821334
5	1.5696709638738895	2.631946005350367
6	1.3509936119478154	2.7183344703869476
7	1.1612372578205	2.725946625284151
8	1.079345684247436	2.895668840084813
9	0.9640555958887869	2.9096646362697367
>10	5.740886296469318	29.180939831900965
>50	0.05430116297103271	1.290101129065302
>100	0.026732880231893026	1.7302203198930086
>500	0.004177012536233285	0.9455138568068174
>1k	0.007518622565219913	6.2235823196928735
>5k	0.0	0.0
>10k+	0.002506207521739971	9.645361948445013
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	12000	3.3542976939203357	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	11344	3.170929419986024	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	11085	3.09853249475891	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3998	1.117540181691125	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	3471	0.9702306079664571	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2825	0.7896575821104124	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	2814	0.7865828092243187	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	2435	0.6806429070580015	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	2248	0.6283717679944095	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	2084	0.5825296995108316	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1315	0.36757512229210343	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	1025	0.286512928022362	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	958	0.26778476589797345	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG	753	0.21048218029350105	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG	605	0.16911250873515024	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT	535	0.14954577218728163	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	524	0.14647099930118798	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	480	0.13417190775681342	Illumina Single End Adapter 1 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCTT	431	0.12047519217330538	Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	372	0.10398322851153041	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.795248078266946E-4	0.0	0.0	0.2948986722571628	0.0
2	2.795248078266946E-4	0.0	0.0	2.0573025856044724	0.0
3	2.795248078266946E-4	0.0	0.0	2.49727463312369	0.0
4	2.795248078266946E-4	0.0	0.0	3.6195667365478688	0.0
5	2.795248078266946E-4	0.0	0.0	8.063172606568832	0.0
6	2.795248078266946E-4	0.0	0.0	9.187421383647798	0.0
7	2.795248078266946E-4	0.0	0.0	10.746610761705101	0.0
8	2.795248078266946E-4	0.0	0.0	12.651292802236199	0.0
9	2.795248078266946E-4	0.0	0.0	13.086792452830188	0.0
10	2.795248078266946E-4	0.0	0.0	17.120894479385047	0.0
11	2.795248078266946E-4	0.0	0.0	18.59874213836478	0.0
12	2.795248078266946E-4	0.0	0.0	23.182669461914745	0.0
13	2.795248078266946E-4	0.0	0.0	23.877288609364083	0.0
14	2.795248078266946E-4	0.0	0.0	24.19119496855346	0.0
15	2.795248078266946E-4	0.0	0.0	25.228511530398322	0.0
16	2.795248078266946E-4	0.0	0.0	26.05814116002795	0.0
17	2.795248078266946E-4	0.0	0.0	26.961844863731656	0.0
18	2.795248078266946E-4	0.0	0.0	27.756813417190777	0.0
19	2.795248078266946E-4	0.0	0.0	29.370510132774285	0.0
20	2.795248078266946E-4	0.0	0.0	30.05590496156534	0.0
21	2.795248078266946E-4	0.0	0.0	30.713906359189377	0.0
22	2.795248078266946E-4	0.0	0.0	31.50719776380154	0.0
23	2.795248078266946E-4	0.0	0.0	32.12606568832984	0.0
24	2.795248078266946E-4	0.0	0.0	32.652410901467505	0.0
25	2.795248078266946E-4	0.0	0.0	33.08986722571628	0.0
26	2.795248078266946E-4	0.0	0.0	33.54409503843466	0.0
27	2.795248078266946E-4	0.0	0.0	34.01872816212439	0.0
28	2.795248078266946E-4	0.0	0.0	34.42711390635919	0.0
29	2.795248078266946E-4	0.0	0.0	34.84025157232704	0.0
30	2.795248078266946E-4	0.0	0.0	35.29643605870021	0.0
31	2.795248078266946E-4	0.0	0.0	35.75513626834382	0.0
32	5.590496156533892E-4	0.0	0.0	36.204053109713485	0.0
33	5.590496156533892E-4	0.0	0.0	36.66135569531796	0.0
34	5.590496156533892E-4	0.0	0.0	37.11027253668763	0.0
35	5.590496156533892E-4	0.0	0.0	37.53487071977638	0.0
36	5.590496156533892E-4	0.0	0.0	37.95611460517121	0.0
37	5.590496156533892E-4	0.0	0.0	38.36310272536688	0.0
38	5.590496156533892E-4	0.0	0.0	38.793850454227815	0.0
39	5.590496156533892E-4	0.0	0.0	39.20139762403913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.026766E-4	45.0	1
TACCAGA	40	6.7921064E-9	45.0	43
TAGACGG	55	1.8189894E-12	45.0	2
ACCGCGT	40	6.7921064E-9	45.0	39
TTAGACG	20	7.026766E-4	45.0	1
GTCTCGT	20	7.026766E-4	45.0	27
GTAGGTA	25	3.885402E-5	45.0	9
CCAGACA	40	6.7921064E-9	45.0	45
CCCCGTA	40	6.7921064E-9	45.0	40
TGTTCTA	20	7.026766E-4	45.0	11
TCGTACG	20	7.026766E-4	45.0	30
CCGAATG	20	7.026766E-4	45.0	44
CCGCGTA	40	6.7921064E-9	45.0	40
ATACGGT	20	7.026766E-4	45.0	28
CGCGTAA	40	6.7921064E-9	45.0	41
ATACGCC	25	3.885402E-5	45.0	28
GACGAAT	25	3.885402E-5	45.0	20
ACCCCGT	40	6.7921064E-9	45.0	39
GATTACG	25	3.885402E-5	45.0	1
TTACACG	25	3.885402E-5	45.0	34
>>END_MODULE