SRR3551940_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639971
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	26211	4.095654334337024	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	22085	3.450937620610934	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	21900	3.4220300607371272	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	8140	1.2719326344474984	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	6422	1.0034829703220929	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	5533	0.8645704258474212	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5111	0.798629937919062	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	4735	0.7398772756890546	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	4391	0.686124840031814	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	4258	0.6653426483387528	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2010	0.3140767316019007	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	1800	0.28126274471812	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATG	1325	0.20704063152861613	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT	1247	0.1948525792574976	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGGATCA	1109	0.17328910216244173	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTAT	1048	0.16375742025810544	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGATCATCGT	1014	0.15844467952454097	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	792	0.12375560767597282	No Hit
TGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCTT	789	0.12328683643477595	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	705	0.11016124168126368	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.8888596E-5	45.000004	16
CGGTTAT	35	1.2107921E-7	45.000004	31
TACGGGT	35	1.2107921E-7	45.000004	4
CACGAAA	35	1.2107921E-7	45.000004	35
ACGGCCA	35	1.2107921E-7	45.000004	30
CGCCCAT	50	2.1827873E-11	45.000004	38
AACGTAG	20	7.0309406E-4	45.0	1
CCAATCG	20	7.0309406E-4	45.0	13
TAAACGG	20	7.0309406E-4	45.0	2
ACGTAAG	20	7.0309406E-4	45.0	28
TACGGCT	3080	0.0	43.90422	7
ACGGCTG	3115	0.0	43.555378	8
TGATACC	2405	0.0	43.316006	4
ATGAATG	1515	0.0	43.217823	21
CGTTTTT	3020	0.0	43.137413	1
GATGAAT	1555	0.0	43.118973	20
CCGATGA	1435	0.0	43.11847	18
GATACCT	2430	0.0	42.87037	5
CGGCTGT	3200	0.0	42.468754	9
CGATGAA	1485	0.0	42.424244	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content