##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639971
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.009375424823936	33.0	31.0	34.0	30.0	34.0
2	32.09816382304823	33.0	31.0	34.0	30.0	34.0
3	32.1064157594641	33.0	31.0	34.0	30.0	34.0
4	35.764619021799426	37.0	35.0	37.0	35.0	37.0
5	35.77875247472151	37.0	35.0	37.0	35.0	37.0
6	35.67597281751829	37.0	35.0	37.0	35.0	37.0
7	35.862335949597714	37.0	35.0	37.0	35.0	37.0
8	35.12468689987515	37.0	35.0	37.0	32.0	37.0
9	36.8194433810282	39.0	37.0	39.0	32.0	39.0
10	36.6393930349969	38.0	35.0	39.0	32.0	39.0
11	37.08446788995126	39.0	37.0	39.0	34.0	39.0
12	37.14361588259468	39.0	37.0	39.0	34.0	39.0
13	37.18646782432329	39.0	37.0	39.0	34.0	39.0
14	38.297608797898654	40.0	38.0	41.0	34.0	41.0
15	38.16554343868707	40.0	37.0	41.0	33.0	41.0
16	38.42283009698877	40.0	38.0	41.0	34.0	41.0
17	38.20898446960878	40.0	37.0	41.0	33.0	41.0
18	38.10783926146654	40.0	37.0	41.0	34.0	41.0
19	38.0723392153707	40.0	37.0	41.0	34.0	41.0
20	37.87545998178042	40.0	36.0	41.0	33.0	41.0
21	38.022683840361516	40.0	37.0	41.0	34.0	41.0
22	38.0880477396632	40.0	37.0	41.0	34.0	41.0
23	38.0142975228565	40.0	37.0	41.0	34.0	41.0
24	37.89814382214194	40.0	36.0	41.0	33.0	41.0
25	37.820463739763206	40.0	36.0	41.0	33.0	41.0
26	37.89827976580189	40.0	36.0	41.0	33.0	41.0
27	37.81884647898108	40.0	36.0	41.0	33.0	41.0
28	37.669189697658176	40.0	36.0	41.0	33.0	41.0
29	37.73775530453724	40.0	36.0	41.0	33.0	41.0
30	37.39529603685167	40.0	36.0	41.0	33.0	41.0
31	37.39304437232312	40.0	36.0	41.0	33.0	41.0
32	37.213564364635275	40.0	36.0	41.0	32.0	41.0
33	36.95072120455458	40.0	35.0	41.0	31.0	41.0
34	36.84250380095348	40.0	35.0	41.0	31.0	41.0
35	36.683524722213974	40.0	35.0	41.0	30.0	41.0
36	36.63007386272191	40.0	35.0	41.0	31.0	41.0
37	36.52399561855147	40.0	35.0	41.0	30.0	41.0
38	36.32658354831703	39.0	35.0	41.0	30.0	41.0
39	36.30732642572867	39.0	35.0	41.0	30.0	41.0
40	36.15213501861803	39.0	35.0	41.0	29.0	41.0
41	35.99620764065872	39.0	35.0	41.0	28.0	41.0
42	36.275946878842944	39.0	35.0	41.0	30.0	41.0
43	36.20446238970203	39.0	35.0	41.0	30.0	41.0
44	36.35584112405093	39.0	35.0	41.0	30.0	41.0
45	36.34002790751456	39.0	35.0	41.0	30.0	41.0
46	36.1966948502354	39.0	35.0	41.0	30.0	41.0
47	35.95778089944701	39.0	35.0	41.0	29.0	41.0
48	35.99704674118046	39.0	35.0	41.0	30.0	41.0
49	36.00559869119069	39.0	35.0	41.0	30.0	41.0
50	35.89186541265151	39.0	35.0	41.0	29.0	41.0
51	35.05900736127106	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	0.0
11	4.0
12	4.0
13	9.0
14	10.0
15	10.0
16	28.0
17	50.0
18	105.0
19	368.0
20	1001.0
21	2190.0
22	3262.0
23	3933.0
24	4528.0
25	5911.0
26	7421.0
27	8044.0
28	7601.0
29	7708.0
30	8723.0
31	10848.0
32	14135.0
33	19263.0
34	33248.0
35	42715.0
36	45735.0
37	72523.0
38	138279.0
39	202244.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.49094724604709	16.746383820516865	20.420612809017907	14.342056124418137
2	28.030488881527443	38.364863407873166	20.349047066195187	13.2556006444042
3	23.815610394846015	37.99062770031767	25.8830478256046	12.310714079231715
4	22.780407237202933	19.34212644010432	43.54166048149057	14.335805841202179
5	34.397808650704484	22.651807660034596	25.145826920282328	17.804556768978593
6	19.34353275382791	43.49431458612968	25.371305887298018	11.79084677274439
7	66.08549449896948	3.2828050021016573	26.21650043517597	4.415200063752889
8	61.87796009506681	16.789667031787378	14.528158307173294	6.80421456597252
9	57.81261963432718	4.583957710583761	14.961302934039198	22.642119721049863
10	40.12009919199463	16.579501258650783	23.972648760646965	19.32775078870761
11	29.644468264968253	18.42474112108205	28.46285222299135	23.46793839095834
12	22.234288741208587	15.287723974992618	34.24155157030553	28.236435713493268
13	23.012917772836584	17.632048952218145	41.62297979127179	17.73205348367348
14	18.939451943916207	27.145448778147756	30.88889965326554	23.026199624670493
15	14.855516890609104	19.75745775980474	42.701934931426585	22.68509041815957
16	16.669349079880185	21.622073500205477	29.740097598172415	31.968479821741923
17	17.38625656475059	24.83815672897678	33.96919547917015	23.806391227102477
18	16.859201432564912	22.343356183327057	33.026809027284045	27.770633356823982
19	17.94112545724728	23.795296974394155	29.359767864481363	28.903809703877208
20	21.92693106406384	23.12229772911585	36.44024494859923	18.510526258221073
21	21.645043291024123	30.013078717629394	29.737284970725238	18.604593020621245
22	18.453648680955855	19.862462517832842	34.17436102573398	27.509527775477327
23	20.706563266148	27.22435860374923	30.327936734633287	21.741141395469484
24	23.329650874805264	22.351481551507803	26.897625048635014	27.421242525051916
25	17.450946996035757	32.81992465283583	25.289739691329764	24.43938865979865
26	19.433380575057306	20.499053863378183	32.32412093672995	27.743444624834563
27	24.60392736545875	25.774761668888125	25.4039636171014	24.217347348551733
28	18.821634105295395	24.2218788038833	32.54850610418284	24.40798098663846
29	27.777508668361534	18.671314793951602	27.999706236688848	25.551470300998012
30	20.233729340860755	32.76335958973141	27.878919513540456	19.123991555867377
31	28.185339648202813	21.181428533480425	24.06218406771557	26.571047750601203
32	31.31782533896067	26.993254381839176	22.24428919435412	19.44463108484603
33	23.618726473543333	26.33338073131439	23.301524600333455	26.746368194808827
34	30.162304229410395	20.3915489920637	27.87985705602285	21.56628972250305
35	22.902912788235717	25.165827826573388	23.60263199426224	28.328627390928652
36	28.9195916689975	29.125694758043725	22.231476113761406	19.72323745919737
37	24.032651479520165	26.081494317711275	29.260857132588818	20.62499707017974
38	24.82518739130367	27.078258233576207	21.64269943481814	26.453854940301984
39	27.92642166598174	24.084685087293018	28.298469774411654	19.69042347231359
40	20.355297349411146	25.056291613213723	27.925796637660145	26.662614399714986
41	20.227947828886	27.733600428769428	27.393272507660505	24.645179234684072
42	21.588634485000103	21.012045858327955	30.04323633414639	27.356083322525553
43	25.55162655807841	26.729023658884543	23.66294722729624	24.056402555740807
44	23.227927515465545	21.650824802998887	30.297935375196687	24.82331230633888
45	22.235851312012574	18.958359050644482	27.711880694593972	31.093908942748964
46	30.563416154794513	24.536580563806798	24.611115191157097	20.28888809024159
47	18.332393186566268	22.885568252311433	37.173090655670336	21.608947905451966
48	23.06088869651906	23.76623315743995	26.57995440418394	26.592923741857057
49	21.07376740508554	18.752568475759055	35.71083689729691	24.46282722185849
50	23.61044484828219	20.07278454804983	30.05354930145272	26.263221302215257
51	21.07564249005033	18.805539626014305	27.379990655826592	32.738827228108775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	190.0
2	333.0
3	4718.5
4	9104.0
5	6004.0
6	2904.0
7	2823.5
8	2743.0
9	2683.5
10	2624.0
11	2585.5
12	2547.0
13	2362.0
14	2177.0
15	2053.5
16	1930.0
17	1929.0
18	1928.0
19	1775.5
20	1623.0
21	1620.5
22	1618.0
23	1691.5
24	1765.0
25	1942.5
26	2591.0
27	3062.0
28	3711.5
29	4361.0
30	4797.5
31	5234.0
32	6148.0
33	7062.0
34	8192.0
35	9322.0
36	10563.5
37	11805.0
38	12826.5
39	13848.0
40	15726.5
41	17605.0
42	19711.0
43	21817.0
44	25915.5
45	30014.0
46	82316.0
47	134618.0
48	99888.5
49	65159.0
50	64548.5
51	63938.0
52	53987.0
53	44036.0
54	40779.0
55	37522.0
56	33856.0
57	30190.0
58	27896.0
59	25602.0
60	23914.5
61	22227.0
62	19976.0
63	17725.0
64	15486.5
65	13248.0
66	11631.5
67	10015.0
68	8336.0
69	6657.0
70	5921.5
71	5186.0
72	4242.5
73	3299.0
74	2388.5
75	1156.0
76	834.0
77	630.0
78	426.0
79	277.0
80	128.0
81	90.0
82	52.0
83	38.5
84	25.0
85	15.0
86	5.0
87	5.5
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.583876991706454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44290403059826	22.155848657938126
2	10.523320967074373	6.436879080024961
3	3.76016220701607	3.4500101522472884
4	1.9382073997231586	2.3711158679019317
5	1.3124600830025173	2.0070058867536913
6	1.0235249515444471	1.8782016729586606
7	0.852554245629792	1.825208992396905
8	0.8342945524090499	2.041276957258334
9	0.7269402886022026	2.000938713023282
>10	6.471291012389117	33.40571433070909
>50	0.06665575812300467	1.3537815755270333
>100	0.03589156206619615	1.8916561039695814
>500	0.0030764196056739562	0.6250635190601634
>1k	0.005127366009456593	3.5968594923941213
>5k	0.002050946403782637	3.952672117769814
>10k+	0.0015382098028369781	11.007766880067042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	26211	4.095654334337024	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	22085	3.450937620610934	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	21900	3.4220300607371272	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	8140	1.2719326344474984	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	6422	1.0034829703220929	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	5533	0.8645704258474212	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5111	0.798629937919062	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	4735	0.7398772756890546	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	4391	0.686124840031814	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	4258	0.6653426483387528	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2010	0.3140767316019007	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	1800	0.28126274471812	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATG	1325	0.20704063152861613	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT	1247	0.1948525792574976	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGGATCA	1109	0.17328910216244173	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTAT	1048	0.16375742025810544	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGATCATCGT	1014	0.15844467952454097	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	792	0.12375560767597282	No Hit
TGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCTT	789	0.12328683643477595	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	705	0.11016124168126368	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.288294313336073	0.0
2	0.0	0.0	0.0	2.146503513440453	0.0
3	0.0	0.0	0.0	2.58527339520072	0.0
4	0.0	0.0	0.0	3.7148558294047698	0.0
5	0.0	0.0	0.0	8.429913230443255	0.0
6	0.0	0.0	0.0	9.500743002417297	0.0
7	0.0	0.0	0.0	11.158161854209018	0.0
8	0.0	0.0	0.0	13.088561825457717	0.0
9	0.0	0.0	0.0	13.547020099348252	0.0
10	0.0	0.0	0.0	17.981127269829415	0.0
11	0.0	0.0	0.0	19.52713482329668	0.0
12	0.0	0.0	0.0	24.885971395578864	0.0
13	0.0	0.0	0.0	25.53709464960131	0.0
14	0.0	0.0	0.0	25.884454139328188	0.0
15	0.0	0.0	0.0	26.9598153666338	0.0
16	0.0	0.0	0.0	27.828917247812793	0.0
17	0.0	0.0	0.0	28.764584645241737	0.0
18	0.0	0.0	0.0	29.57399632170833	0.0
19	0.0	0.0	0.0	31.28485509499649	0.0
20	0.0	0.0	0.0	31.998949952419718	0.0
21	0.0	0.0	0.0	32.73101437408883	0.0
22	0.0	0.0	0.0	33.513237318566	0.0
23	0.0	0.0	0.0	34.188111648809084	0.0
24	0.0	0.0	0.0	34.74673071123535	0.0
25	0.0	0.0	0.0	35.23128391755252	0.0
26	0.0	0.0	0.0	35.720212322120844	0.0
27	0.0	0.0	0.0	36.21554726698554	0.0
28	0.0	0.0	0.0	36.67103665634849	0.0
29	0.0	0.0	0.0	37.12527598906826	0.0
30	0.0	0.0	0.0	37.632173957882465	0.0
31	1.5625708039895556E-4	0.0	0.0	38.11953979164681	0.0
32	1.5625708039895556E-4	0.0	0.0	38.5847171199945	0.0
33	1.5625708039895556E-4	0.0	0.0	39.06411384265849	0.0
34	1.5625708039895556E-4	0.0	0.0	39.52413468735302	0.0
35	1.5625708039895556E-4	0.0	0.0	40.01900086097651	0.0
36	1.5625708039895556E-4	0.0	0.0	40.503241553132874	0.0
37	1.5625708039895556E-4	0.0	0.0	40.96982519520416	0.0
38	1.5625708039895556E-4	0.0	0.0	41.439690235963816	0.0
39	1.5625708039895556E-4	0.0	0.0	41.881429002251664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.8888596E-5	45.000004	16
CGGTTAT	35	1.2107921E-7	45.000004	31
TACGGGT	35	1.2107921E-7	45.000004	4
CACGAAA	35	1.2107921E-7	45.000004	35
ACGGCCA	35	1.2107921E-7	45.000004	30
CGCCCAT	50	2.1827873E-11	45.000004	38
AACGTAG	20	7.0309406E-4	45.0	1
CCAATCG	20	7.0309406E-4	45.0	13
TAAACGG	20	7.0309406E-4	45.0	2
ACGTAAG	20	7.0309406E-4	45.0	28
TACGGCT	3080	0.0	43.90422	7
ACGGCTG	3115	0.0	43.555378	8
TGATACC	2405	0.0	43.316006	4
ATGAATG	1515	0.0	43.217823	21
CGTTTTT	3020	0.0	43.137413	1
GATGAAT	1555	0.0	43.118973	20
CCGATGA	1435	0.0	43.11847	18
GATACCT	2430	0.0	42.87037	5
CGGCTGT	3200	0.0	42.468754	9
CGATGAA	1485	0.0	42.424244	19
>>END_MODULE