SRR3551939_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435860
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	18978	4.354150415270959	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	16423	3.7679530124351857	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	15887	3.644977745147524	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	5677	1.3024824484926354	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT	5055	1.1597760748864314	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	4045	0.9280502913779654	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3821	0.8766576423622264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA	3796	0.8709218556417199	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	3667	0.8413251961639059	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC	3190	0.7318863855366402	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	1369	0.31409168081494054	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1348	0.30927361996971503	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT	1157	0.26545220942504477	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATG	1113	0.25535722479695316	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGT	936	0.21474785481576655	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGGTTCT	911	0.20901206809525993	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTAT	837	0.19203413940256045	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	547	0.12549901344468406	No Hit
TGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCTT	531	0.12182810994355986	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	519	0.1190749323177167	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	506	0.11609232322305327	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCATA	30	2.1623891E-6	45.000004	30
GTTACGG	30	2.1623891E-6	45.000004	2
GTTACCG	30	2.1623891E-6	45.000004	38
CCGGTAT	30	2.1623891E-6	45.000004	42
CGGTATT	30	2.1623891E-6	45.000004	43
TCACCCG	40	6.7975634E-9	45.0	37
CGACCGA	20	7.028462E-4	45.0	41
ATAGACG	20	7.028462E-4	45.0	1
ACTAGGC	20	7.028462E-4	45.0	3
GTATGCG	20	7.028462E-4	45.0	1
GCGATAT	20	7.028462E-4	45.0	9
CCGCGTA	20	7.028462E-4	45.0	40
CAGTACG	25	3.886808E-5	45.0	1
CCGTTTA	20	7.028462E-4	45.0	40
CGTTTTT	2150	0.0	44.267445	1
CATCGAA	215	0.0	43.953487	42
CCATCGA	200	0.0	43.875	41
CGAATGC	340	0.0	43.67647	45
TACGGCT	2215	0.0	43.374714	7
TGATACC	1780	0.0	43.35674	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content