##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	435860
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89633597944294	33.0	31.0	34.0	30.0	34.0
2	31.99214885513697	33.0	31.0	34.0	30.0	34.0
3	32.007330335428804	33.0	31.0	34.0	30.0	34.0
4	35.70153489652641	37.0	35.0	37.0	35.0	37.0
5	35.68150782361309	37.0	35.0	37.0	35.0	37.0
6	35.558706924241726	37.0	35.0	37.0	33.0	37.0
7	35.72742394346809	37.0	35.0	37.0	35.0	37.0
8	34.80783279034552	37.0	35.0	37.0	32.0	37.0
9	36.42096085899142	39.0	35.0	39.0	32.0	39.0
10	36.40723626852659	37.0	35.0	39.0	32.0	39.0
11	36.85347818106732	39.0	37.0	39.0	33.0	39.0
12	36.91178818886799	39.0	37.0	39.0	33.0	39.0
13	36.92865140182627	39.0	37.0	39.0	33.0	39.0
14	38.04331207268389	40.0	37.0	41.0	33.0	41.0
15	37.902487037122015	40.0	37.0	41.0	33.0	41.0
16	38.1907424402331	40.0	37.0	41.0	34.0	41.0
17	37.88972835314092	40.0	37.0	41.0	33.0	41.0
18	37.80214747854816	39.0	37.0	41.0	33.0	41.0
19	37.73413481393108	39.0	37.0	41.0	33.0	41.0
20	37.36743679163034	39.0	35.0	41.0	32.0	41.0
21	37.62323223053274	39.0	36.0	41.0	33.0	41.0
22	37.617062818336166	40.0	36.0	41.0	33.0	41.0
23	37.50116092323223	40.0	35.0	41.0	32.0	41.0
24	37.40605240214748	39.0	35.0	41.0	32.0	41.0
25	37.44795576561281	39.0	35.0	41.0	33.0	41.0
26	37.39952966548892	39.0	35.0	41.0	32.0	41.0
27	37.19830220713073	39.0	35.0	41.0	32.0	41.0
28	37.04248382508145	39.0	35.0	41.0	31.0	41.0
29	37.0747763043179	39.0	35.0	41.0	31.0	41.0
30	36.631404579452116	39.0	35.0	41.0	30.0	41.0
31	36.71140044968568	39.0	35.0	41.0	30.0	41.0
32	36.40284724452806	39.0	35.0	41.0	30.0	41.0
33	35.99146973798926	39.0	35.0	41.0	27.0	41.0
34	35.861405038315056	39.0	35.0	41.0	27.0	41.0
35	35.5441839122654	39.0	35.0	41.0	25.0	41.0
36	35.4678910659386	39.0	35.0	41.0	25.0	41.0
37	35.30217959895379	39.0	35.0	41.0	23.0	41.0
38	35.132136465837654	39.0	35.0	40.0	23.0	41.0
39	35.125508190703435	39.0	35.0	40.0	23.0	41.0
40	35.031037489102005	38.0	35.0	40.0	23.0	41.0
41	34.95978066351581	38.0	34.0	40.0	23.0	41.0
42	35.1311544991511	39.0	35.0	40.0	23.0	41.0
43	34.95670628183362	39.0	35.0	40.0	22.0	41.0
44	35.298676180424906	39.0	35.0	41.0	23.0	41.0
45	35.25457945211765	39.0	35.0	40.0	23.0	41.0
46	35.16337814894691	39.0	35.0	40.0	23.0	41.0
47	34.819063460744275	38.0	35.0	40.0	23.0	41.0
48	34.94375028678934	38.0	35.0	40.0	23.0	41.0
49	34.94941265543982	38.0	35.0	40.0	23.0	41.0
50	34.737135777543244	38.0	35.0	40.0	23.0	41.0
51	34.073415775707794	37.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	2.0
11	3.0
12	2.0
13	6.0
14	12.0
15	16.0
16	29.0
17	63.0
18	142.0
19	488.0
20	1381.0
21	2706.0
22	3897.0
23	4495.0
24	5066.0
25	6013.0
26	6672.0
27	6626.0
28	6368.0
29	6085.0
30	7089.0
31	8829.0
32	11131.0
33	14778.0
34	24466.0
35	29734.0
36	33343.0
37	51227.0
38	92766.0
39	112383.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.60647914467948	14.16280457027486	18.192309457165145	16.03840682788051
2	26.32221355481118	41.71683568118203	18.637865369614097	13.323085394392695
3	22.387463864543662	40.93653925572431	24.763915018583948	11.912081861148074
4	21.713394209149726	18.085853255632543	46.31533061074657	13.885421924471162
5	37.573532785756896	20.962465011701003	24.002890836507135	17.461111366034963
6	19.076308906529622	43.72872023126692	24.882301656495205	12.312669205708254
7	61.3644289450741	3.564906162529252	30.445555912448953	4.62510897994769
8	57.64580369843528	20.282659569586563	15.120680952599457	6.950855779378699
9	54.40921396778782	4.497086220345983	15.041068232918828	26.052631578947366
10	43.747304180241365	14.933006011104483	23.424723535080073	17.894966273574084
11	33.01840040379938	17.546230440967285	27.411554168769786	22.023814986463545
12	21.103565365025467	14.346120313862249	33.57591887303262	30.97439544807966
13	25.4563391914835	16.910705272335154	41.02716468590832	16.60579085027302
14	19.10429954572569	29.01459184141697	29.708622034598264	22.172486578259072
15	14.18964805212683	18.37310145459551	42.202771532143345	25.23447896113431
16	16.14050383150553	20.253062910108753	28.4160051392649	35.19042811912082
17	17.147019685220023	26.178360023860876	33.56628275134217	23.10833753957693
18	16.75882163997614	20.895471022805488	32.13646583765429	30.209241499564083
19	17.236039095122287	22.614142155738083	28.868214564309643	31.28160418482999
20	23.264809801312346	22.250493277657963	36.34309181847382	18.141605102555864
21	20.14247694213738	32.307162850456564	28.615151654200886	18.935208553205158
22	17.879594365163126	19.0976460331299	35.895012159867846	27.127747441839123
23	23.220300096361218	26.43807644656541	29.431239388794566	20.910384068278805
24	22.497820401046205	23.69430551094388	26.147386775570137	27.660487312439773
25	16.74574404625338	34.807736429128624	24.926811361446337	23.51970816317166
26	19.237369797641442	19.12127747441839	34.149038682145644	27.49231404579452
27	26.700775478364612	22.652916073968708	26.722342036433716	23.923966411232964
28	18.393520855320517	24.906391960721333	32.647180287248204	24.052906896709953
29	27.512045152113064	16.16757674482632	31.139356674161426	25.181021428899186
30	19.937365209012068	27.244528059468635	34.15546276327261	18.662643968246684
31	28.8133804432616	17.914926811361447	25.162437479924748	28.10925526545221
32	32.91125590786032	23.184738218694076	24.48767953012435	19.41632634332125
33	24.879089615931722	16.69733400633231	31.113430918184736	27.310145459551233
34	30.17299132749048	17.281466525948698	29.271555086495667	23.273987060065156
35	24.453494241270132	17.730005047492313	30.416876978846418	27.399623732391138
36	29.458541733584177	21.492910567613453	29.621438076446566	19.4271096223558
37	23.533244619832054	18.163859955031434	36.23892993162942	22.06396549350709
38	25.632771991006287	19.49295645390722	27.36130867709815	27.512962877988343
39	29.081356398843667	20.3475886752627	31.224246317624925	19.34680860826871
40	20.1743679163034	17.76877896572294	33.627311522048366	28.429541595925294
41	20.141559216262102	21.144863029413113	32.660028449502136	26.05354930482265
42	22.276877896572294	17.600146836140045	32.286284586793926	27.836690680493735
43	25.571054925893637	18.8496306153352	30.79039141008581	24.78892304868536
44	23.21548203551599	17.436332767402376	34.508557793787	24.839627403294635
45	22.724269260771806	15.973248290735556	29.55536181342633	31.747120635066306
46	31.981140733262976	21.117560684623506	26.94213738356353	19.959161198549992
47	17.861469279126325	20.29642545771578	40.818840912219514	21.023264350938376
48	23.2416372229615	21.678979488826688	28.563070710778693	26.51631257743312
49	21.904281191208185	16.08062221814344	37.67172945441197	24.343367136236406
50	24.22520992979397	16.878126003762674	32.85160372596705	26.0450603404763
51	21.884550084889643	16.60602028174184	29.481943743403843	32.027485889964666
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	149.0
2	240.0
3	3485.0
4	6730.0
5	4473.5
6	2217.0
7	2157.0
8	2097.0
9	2104.5
10	2112.0
11	1996.5
12	1881.0
13	1798.5
14	1716.0
15	1616.5
16	1517.0
17	1399.0
18	1281.0
19	1220.5
20	1160.0
21	1137.0
22	1114.0
23	1188.5
24	1263.0
25	1426.5
26	1660.5
27	1731.0
28	2105.0
29	2479.0
30	2815.0
31	3151.0
32	3727.0
33	4303.0
34	4892.0
35	5481.0
36	6131.0
37	6781.0
38	7621.5
39	8462.0
40	9486.0
41	10510.0
42	11980.5
43	13451.0
44	15727.0
45	18003.0
46	55844.0
47	93685.0
48	67339.5
49	40994.0
50	41152.0
51	41310.0
52	35233.5
53	29157.0
54	27183.0
55	25209.0
56	23961.0
57	22713.0
58	20615.5
59	18518.0
60	17079.5
61	15641.0
62	14326.0
63	13011.0
64	12036.0
65	11061.0
66	9666.0
67	8271.0
68	7197.0
69	6123.0
70	5131.0
71	4139.0
72	3568.5
73	2998.0
74	2392.0
75	1381.0
76	976.0
77	713.5
78	451.0
79	352.0
80	253.0
81	203.0
82	153.0
83	106.0
84	59.0
85	36.5
86	14.0
87	9.0
88	4.0
89	4.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	435860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.61412032360274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59490515573434	26.210178074204542
2	8.671961985166032	6.17688595162824
3	3.7065912329492967	3.960209584820018
4	2.5479923897811276	3.6297803021315667
5	1.9942311119390863	3.551139338683535
6	1.7384958649722622	3.714900055032478
7	1.5513779136482198	3.8675671778833234
8	1.2980706566976334	3.698371564493404
9	1.089695210431433	3.492768270632279
>10	3.7277456217454032	18.283838758469862
>50	0.03881943783696019	0.9567039299739211
>100	0.026526615855256126	1.6477335749623094
>500	0.004528934414312022	1.1030206437343835
>1k	0.005822915675544028	5.416253029375479
>5k	0.0012939812612320063	2.4728676725626495
>10k+	0.0019409718918480096	11.817782071411962
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	18978	4.354150415270959	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	16423	3.7679530124351857	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	15887	3.644977745147524	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	5677	1.3024824484926354	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGTTCTT	5055	1.1597760748864314	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	4045	0.9280502913779654	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3821	0.8766576423622264	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA	3796	0.8709218556417199	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	3667	0.8413251961639059	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC	3190	0.7318863855366402	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	1369	0.31409168081494054	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1348	0.30927361996971503	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTT	1157	0.26545220942504477	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATG	1113	0.25535722479695316	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGT	936	0.21474785481576655	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGGTTCT	911	0.20901206809525993	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTAT	837	0.19203413940256045	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTG	547	0.12549901344468406	No Hit
TGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCTT	531	0.12182810994355986	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	519	0.1190749323177167	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCC	506	0.11609232322305327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32418666544303215	0.0
2	0.0	0.0	0.0	2.146101959344744	0.0
3	0.0	0.0	0.0	2.5577020144082963	0.0
4	0.0	0.0	0.0	3.8028265956958656	0.0
5	0.0	0.0	0.0	8.622493461203138	0.0
6	0.0	0.0	0.0	9.603083558940945	0.0
7	0.0	0.0	0.0	11.26324966732437	0.0
8	0.0	0.0	0.0	13.068875326939843	0.0
9	0.0	0.0	0.0	13.506630569448905	0.0
10	0.0	0.0	0.0	18.070710778690405	0.0
11	0.0	0.0	0.0	19.406001927224338	0.0
12	0.0	0.0	0.0	24.958243472674713	0.0
13	0.0	0.0	0.0	25.641490386821456	0.0
14	0.0	0.0	0.0	26.014316523654383	0.0
15	0.0	0.0	0.0	27.191299958702334	0.0
16	0.0	0.0	0.0	27.994768962510896	0.0
17	0.0	0.0	0.0	28.769788464185748	0.0
18	0.0	0.0	0.0	29.46129491121002	0.0
19	0.0	0.0	0.0	31.21277474418391	0.0
20	0.0	0.0	0.0	31.876061120543294	0.0
21	0.0	0.0	0.0	32.44390400587345	0.0
22	0.0	0.0	0.0	33.11063185426513	0.0
23	0.0	0.0	0.0	33.673197815812415	0.0
24	0.0	0.0	0.0	34.15385674299087	0.0
25	0.0	0.0	0.0	34.56775111274263	0.0
26	0.0	0.0	0.0	34.99380535034185	0.0
27	2.294314688202634E-4	0.0	0.0	35.485706419492494	0.0
28	2.294314688202634E-4	0.0	0.0	35.85692653604369	0.0
29	2.294314688202634E-4	0.0	0.0	36.234800165190656	0.0
30	2.294314688202634E-4	0.0	0.0	36.63125774331207	0.0
31	2.294314688202634E-4	0.0	0.0	37.00247785986326	0.0
32	2.294314688202634E-4	0.0	0.0	37.39916486945349	0.0
33	2.294314688202634E-4	0.0	0.0	37.7887395035103	0.0
34	2.294314688202634E-4	0.0	0.0	38.166154269719634	0.0
35	2.294314688202634E-4	0.0	0.0	38.5529757261506	0.0
36	2.294314688202634E-4	0.0	0.0	38.91937778185656	0.0
37	2.294314688202634E-4	0.0	0.0	39.287844720781905	0.0
38	2.294314688202634E-4	0.0	0.0	39.652181893268484	0.0
39	2.294314688202634E-4	0.0	0.0	40.03074381682192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCATA	30	2.1623891E-6	45.000004	30
GTTACGG	30	2.1623891E-6	45.000004	2
GTTACCG	30	2.1623891E-6	45.000004	38
CCGGTAT	30	2.1623891E-6	45.000004	42
CGGTATT	30	2.1623891E-6	45.000004	43
TCACCCG	40	6.7975634E-9	45.0	37
CGACCGA	20	7.028462E-4	45.0	41
ATAGACG	20	7.028462E-4	45.0	1
ACTAGGC	20	7.028462E-4	45.0	3
GTATGCG	20	7.028462E-4	45.0	1
GCGATAT	20	7.028462E-4	45.0	9
CCGCGTA	20	7.028462E-4	45.0	40
CAGTACG	25	3.886808E-5	45.0	1
CCGTTTA	20	7.028462E-4	45.0	40
CGTTTTT	2150	0.0	44.267445	1
CATCGAA	215	0.0	43.953487	42
CCATCGA	200	0.0	43.875	41
CGAATGC	340	0.0	43.67647	45
TACGGCT	2215	0.0	43.374714	7
TGATACC	1780	0.0	43.35674	4
>>END_MODULE