SRR3551937_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	18742	3.7044504004490717	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	16578	3.2767249353668078	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	15618	3.086976115367283	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	5786	1.1436319505388075	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTAGGAT	4473	0.8841109081852897	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCT	4231	0.8362783931437425	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTA	3994	0.7894341532063597	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3960	0.7827138824980432	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	3606	0.7127440051232181	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTC	3526	0.696931603456591	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	1979	0.39115928622818874	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1928	0.381078880165714	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATG	1152	0.22769858399943077	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTT	1056	0.20872370199947818	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGT	912	0.18026137899954936	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTAT	887	0.17532000347872836	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTAGGA	788	0.15575215641627727	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	706	0.13954444470798447	No Hit
TGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCTT	694	0.13717258445799038	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	615	0.12155783781219609	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	598	0.11819770245803785	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGCG	35	1.2101191E-7	45.000004	38
TCGTTAG	20	7.029538E-4	45.0	1
CCGATTC	20	7.029538E-4	45.0	11
TACGGGT	20	7.029538E-4	45.0	4
TCGATAG	20	7.029538E-4	45.0	1
TGTTGCG	25	3.887699E-5	45.0	1
GTAGATT	20	7.029538E-4	45.0	8
TATAGCG	20	7.029538E-4	45.0	1
ACCGTAA	20	7.029538E-4	45.0	41
ACTCATA	20	7.029538E-4	45.0	14
TGCGATC	30	2.163084E-6	44.999996	32
CCAACGA	65	0.0	44.999996	37
CGTTTTT	2550	0.0	43.941177	1
CGATCGA	195	0.0	43.846153	41
TGATACC	1620	0.0	43.055553	4
TACGGCT	1935	0.0	42.90698	7
GATACCT	1650	0.0	42.409092	5
TGAATGA	1415	0.0	42.296818	22
GATGAAT	1445	0.0	42.19723	20
AACGATC	80	0.0	42.1875	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content