##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551937_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 505932 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99233889139252 33.0 31.0 34.0 30.0 34.0 2 32.09196097499269 33.0 31.0 34.0 30.0 34.0 3 32.10492912090953 33.0 31.0 34.0 30.0 34.0 4 35.75199236261 37.0 35.0 37.0 35.0 37.0 5 35.76106868116664 37.0 35.0 37.0 35.0 37.0 6 35.6434165065661 37.0 35.0 37.0 35.0 37.0 7 35.817048536166915 37.0 35.0 37.0 35.0 37.0 8 35.09995216748496 37.0 35.0 37.0 32.0 37.0 9 36.7656404417985 39.0 37.0 39.0 32.0 39.0 10 36.63996940300277 38.0 35.0 39.0 32.0 39.0 11 37.04482618217468 39.0 37.0 39.0 34.0 39.0 12 37.06842421511191 39.0 37.0 39.0 34.0 39.0 13 37.14529027616359 39.0 37.0 39.0 33.0 39.0 14 38.25203584671458 40.0 38.0 41.0 34.0 41.0 15 38.133154257884456 40.0 37.0 41.0 33.0 41.0 16 38.37209150636844 40.0 38.0 41.0 34.0 41.0 17 38.16686629823771 40.0 37.0 41.0 33.0 41.0 18 38.086276416593535 40.0 37.0 41.0 34.0 41.0 19 38.01939786374454 40.0 37.0 41.0 34.0 41.0 20 37.831910217183335 40.0 36.0 41.0 33.0 41.0 21 37.97905647399255 40.0 36.0 41.0 34.0 41.0 22 38.027001652395974 40.0 37.0 41.0 34.0 41.0 23 37.97170766031799 40.0 36.0 41.0 34.0 41.0 24 37.8900227698584 40.0 36.0 41.0 33.0 41.0 25 37.793826047769265 40.0 36.0 41.0 33.0 41.0 26 37.79662286631405 40.0 36.0 41.0 33.0 41.0 27 37.75544144272353 40.0 36.0 41.0 33.0 41.0 28 37.608542254690356 40.0 36.0 41.0 33.0 41.0 29 37.67578053967727 40.0 36.0 41.0 33.0 41.0 30 37.408612224567726 40.0 36.0 41.0 33.0 41.0 31 37.35866677735348 40.0 36.0 41.0 33.0 41.0 32 37.130501727504885 40.0 36.0 41.0 32.0 41.0 33 36.92352529588957 40.0 36.0 41.0 31.0 41.0 34 36.718304040859245 40.0 35.0 41.0 31.0 41.0 35 36.713028628353214 40.0 35.0 41.0 31.0 41.0 36 36.64431583691089 40.0 35.0 41.0 30.0 41.0 37 36.49149688100377 40.0 35.0 41.0 30.0 41.0 38 36.41947732106291 40.0 35.0 41.0 30.0 41.0 39 36.23832847101982 39.0 35.0 41.0 30.0 41.0 40 36.08195370128792 39.0 35.0 41.0 29.0 41.0 41 35.92280385506353 39.0 35.0 41.0 27.0 41.0 42 36.12064664816616 39.0 35.0 41.0 29.0 41.0 43 36.075573397215436 39.0 35.0 41.0 29.0 41.0 44 36.22119771036424 39.0 35.0 41.0 30.0 41.0 45 36.23870006245899 39.0 35.0 41.0 30.0 41.0 46 36.151263410893165 39.0 35.0 41.0 30.0 41.0 47 35.9224421463754 39.0 35.0 41.0 29.0 41.0 48 35.89403121368089 39.0 35.0 41.0 29.0 41.0 49 35.96245147569238 39.0 35.0 41.0 29.0 41.0 50 35.84281286813248 39.0 35.0 41.0 29.0 41.0 51 34.8774696994853 38.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 4.0 11 6.0 12 7.0 13 10.0 14 17.0 15 24.0 16 30.0 17 52.0 18 110.0 19 271.0 20 690.0 21 1566.0 22 2793.0 23 3553.0 24 4178.0 25 5173.0 26 6281.0 27 6550.0 28 6156.0 29 6132.0 30 6583.0 31 8260.0 32 11004.0 33 15284.0 34 26886.0 35 33729.0 36 35686.0 37 56804.0 38 106676.0 39 161378.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.390629570772354 16.25356767312603 18.925665899765185 15.430136856336423 2 27.97253385830507 37.72997161673901 20.242641303574395 14.05485322138153 3 24.887336638125284 37.27912051421931 25.355581382478277 12.477961465177138 4 23.05151680462987 19.843180506471224 42.12799348529052 14.97730920360839 5 34.64220488128839 22.56350655819359 25.04723954997905 17.747049010538966 6 20.073053295699815 42.11395997881138 25.508368713582065 12.304618011906738 7 65.18939304096203 3.9056632116569028 26.142841330455475 4.762102416925595 8 61.04338132397239 16.46426792533384 15.157175272566272 7.335175478127495 9 56.9199022793577 5.051271712404039 15.740455239043982 22.28837076919428 10 41.095443656459764 16.5079496849379 24.281721654293463 18.114885004308878 11 30.497972059486255 18.94543140184847 28.09330107603393 22.46329546263134 12 22.307740961235897 15.771289422293904 33.724097309519856 28.196872306950343 13 24.464157238522173 17.536744068372826 40.475399856107146 17.52369883699786 14 19.069361099910658 27.493418087806266 30.291817872757605 23.145402939525468 15 14.76562067629642 20.43515729386558 41.80917593668714 22.99004609315086 16 17.000308341832497 22.120364001486365 29.613861151300963 31.265466505380168 17 17.698425875414088 25.183423859332876 33.74485108670731 23.373299178545732 18 17.172070554936237 22.48306096471463 32.74293778610564 27.601930694243492 19 18.471652316912156 23.86368128523201 28.935113809760992 28.72955258809484 20 22.578923649818552 23.45730256239969 35.675150020160814 18.288623767620944 21 21.556454227050274 30.391436003257354 29.01259457792747 19.039515191764902 22 18.233478016808583 20.227421866970268 34.3467106251433 27.19238949107785 23 21.488856209925444 26.822972257141274 30.646806290173384 21.041365242759895 24 22.87935928148447 22.81472608967213 26.985247029245034 27.320667599598362 25 17.69526339508076 32.76388921831392 25.203782326478656 24.33706506012666 26 18.36155847030826 22.19231042906952 32.17547022129456 27.270660879327657 27 26.36737743412158 24.185068349106203 25.21030494216614 24.237249274606075 28 16.03417060000158 25.559363708956933 32.11004640939889 26.296419281642592 29 22.14981459959046 23.145007629483803 29.56484270613442 25.140335064791312 30 23.699034652878254 28.231066625554423 27.66913340132666 20.400765320240666 31 23.935825367835996 21.670501174070825 23.656934133440856 30.736739324652323 32 27.39834602278567 27.2811365954318 26.474901765454646 18.845615616327883 33 24.019038131606617 24.98754773368753 23.24917182546271 27.744242309243138 34 28.311907529075054 23.95381197473178 24.96639864645842 22.767881849734746 35 29.433006807238915 22.836665796984576 24.54460283200114 23.18572456377537 36 22.82796897606793 33.31495141639588 21.287643398717616 22.569436208818576 37 26.68757856787078 21.459603266842183 31.02017662452662 20.83264154076042 38 21.59302040590435 26.432801246017252 22.57180806906857 29.402370279009826 39 25.95744092091427 24.959678375750098 28.902500731323578 20.180379972012048 40 25.614311804748464 24.834562747562913 27.2137362333278 22.337389214360822 41 25.413889613623965 27.54876149363946 22.399848200944 24.637500691792575 42 26.661092795079185 21.225579722176104 30.288260082382614 21.825067400362105 43 23.866250800502833 27.7331736280765 24.613386779250966 23.7871887921697 44 24.262549117272677 20.621545978510948 29.837804289904575 25.278100614311803 45 22.376327253464893 18.6928282852241 27.523659305993693 31.407185155317315 46 29.45237699928054 24.408418522647313 25.551655163144453 20.587549314927696 47 18.833558660057083 23.31854873777504 36.288671204825945 21.559221397341936 48 23.601195417565997 23.840555647794563 25.688234782539947 26.870014152099493 49 22.78053177106805 18.733940529557334 35.4800645145988 23.00546318477582 50 23.59743997217017 20.15330123415795 29.8783235691753 26.370935224496574 51 21.280330162946797 19.49609828988876 27.40803111880648 31.815540428357963 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 154.0 2 257.0 3 3800.0 4 7343.0 5 4891.0 6 2439.0 7 2380.0 8 2321.0 9 2261.5 10 2202.0 11 2158.5 12 2115.0 13 1950.5 14 1786.0 15 1676.0 16 1566.0 17 1521.5 18 1477.0 19 1414.5 20 1352.0 21 1359.5 22 1367.0 23 1448.0 24 1529.0 25 1569.5 26 1877.0 27 2144.0 28 2556.0 29 2968.0 30 3454.0 31 3940.0 32 4687.5 33 5435.0 34 6163.5 35 6892.0 36 7712.0 37 8532.0 38 9250.5 39 9969.0 40 11571.5 41 13174.0 42 15257.5 43 17341.0 44 20344.5 45 23348.0 46 63492.5 47 103637.0 48 78461.0 49 53285.0 50 52758.5 51 52232.0 52 44697.5 53 37163.0 54 33660.0 55 30157.0 56 26827.0 57 23497.0 58 21321.0 59 19145.0 60 18334.0 61 17523.0 62 15998.5 63 14474.0 64 13052.0 65 11630.0 66 9740.5 67 7851.0 68 6607.5 69 5364.0 70 4599.0 71 3834.0 72 3100.0 73 2366.0 74 1804.5 75 922.5 76 602.0 77 518.5 78 435.0 79 302.0 80 169.0 81 141.5 82 114.0 83 72.0 84 30.0 85 24.5 86 19.0 87 10.0 88 1.0 89 1.0 90 1.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 505932.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.80904020737945 #Duplication Level Percentage of deduplicated Percentage of total 1 72.74288530501417 25.321100193830304 2 9.696216665739744 6.750319915543949 3 3.6992233556634844 3.8629924357010204 4 2.2751685068460263 3.1678572813346717 5 1.7699332077549719 3.080483809655945 6 1.490351237723364 3.1126617694218184 7 1.2866174168646238 3.135014217660877 8 1.1598495197164862 3.2298598853056726 9 1.0198506246356442 3.195001925861687 >10 4.769882487624706 23.97111015958517 >50 0.04672010846945592 1.1064668325289775 >100 0.03133665811975696 1.8549532684430021 >500 0.003988301942514521 1.031300865594313 >1k 0.005697574203592173 5.930971612614987 >5k 5.697574203592173E-4 1.1475205400632107 >10k+ 0.0017092722610776519 10.102385286854446 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC 18742 3.7044504004490717 No Hit GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC 16578 3.2767249353668078 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG 15618 3.086976115367283 No Hit GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC 5786 1.1436319505388075 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTAGGAT 4473 0.8841109081852897 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCT 4231 0.8362783931437425 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTA 3994 0.7894341532063597 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.7827138824980432 No Hit GAACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT 3606 0.7127440051232181 No Hit GAATGATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTC 3526 0.696931603456591 No Hit CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT 1979 0.39115928622818874 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC 1928 0.381078880165714 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATG 1152 0.22769858399943077 No Hit GAATGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTT 1056 0.20872370199947818 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGT 912 0.18026137899954936 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTAT 887 0.17532000347872836 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCTAGGA 788 0.15575215641627727 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC 706 0.13954444470798447 No Hit TGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCTT 694 0.13717258445799038 Illumina Single End Adapter 1 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC 615 0.12155783781219609 No Hit CGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG 598 0.11819770245803785 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.4000537621656665 0.0 2 0.0 0.0 0.0 2.1870528055153655 0.0 3 0.0 0.0 0.0 2.6831669078057923 0.0 4 0.0 0.0 0.0 3.932148984448503 0.0 5 0.0 0.0 0.0 8.543835930520308 0.0 6 0.0 0.0 0.0 9.680747610350798 0.0 7 1.976550208328392E-4 0.0 0.0 11.397381466284006 0.0 8 1.976550208328392E-4 0.0 0.0 13.476514630424642 0.0 9 3.953100416656784E-4 0.0 0.0 13.981523208652547 0.0 10 3.953100416656784E-4 0.0 0.0 18.146312152621302 0.0 11 3.953100416656784E-4 0.0 0.0 19.740202240617315 0.0 12 3.953100416656784E-4 0.0 0.0 24.77585920637556 0.0 13 3.953100416656784E-4 0.0 0.0 25.46508226401967 0.0 14 3.953100416656784E-4 0.0 0.0 25.836476048164574 0.0 15 3.953100416656784E-4 0.0 0.0 26.99236260999502 0.0 16 3.953100416656784E-4 0.0 0.0 27.894262470055263 0.0 17 3.953100416656784E-4 0.0 0.0 28.797545915261338 0.0 18 3.953100416656784E-4 0.0 0.0 29.605757295446818 0.0 19 5.929650624985176E-4 0.0 0.0 31.13936260208882 0.0 20 5.929650624985176E-4 0.0 0.0 31.867523698836997 0.0 21 5.929650624985176E-4 0.0 0.0 32.59133638512686 0.0 22 5.929650624985176E-4 0.0 0.0 33.38867673916653 0.0 23 5.929650624985176E-4 0.0 0.0 34.0782951068523 0.0 24 5.929650624985176E-4 0.0 0.0 34.64675094676755 0.0 25 5.929650624985176E-4 0.0 0.0 35.14582987437047 0.0 26 5.929650624985176E-4 0.0 0.0 35.62850343524426 0.0 27 5.929650624985176E-4 0.0 0.0 36.13351201347216 0.0 28 5.929650624985176E-4 0.0 0.0 36.594838832096016 0.0 29 5.929650624985176E-4 0.0 0.0 37.045294624574055 0.0 30 5.929650624985176E-4 0.0 0.0 37.543187622051974 0.0 31 5.929650624985176E-4 0.0 0.0 38.02645414798827 0.0 32 5.929650624985176E-4 0.0 0.0 38.45734209340386 0.0 33 5.929650624985176E-4 0.0 0.0 38.934876623735995 0.0 34 5.929650624985176E-4 0.0 0.0 39.39916826767234 0.0 35 5.929650624985176E-4 0.0 0.0 39.88441134381696 0.0 36 5.929650624985176E-4 0.0 0.0 40.34870298775329 0.0 37 5.929650624985176E-4 0.0 0.0 40.80666967102298 0.0 38 5.929650624985176E-4 0.0 0.0 41.275902690480144 0.0 39 5.929650624985176E-4 0.0 0.0 41.73584592395816 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGCG 35 1.2101191E-7 45.000004 38 TCGTTAG 20 7.029538E-4 45.0 1 CCGATTC 20 7.029538E-4 45.0 11 TACGGGT 20 7.029538E-4 45.0 4 TCGATAG 20 7.029538E-4 45.0 1 TGTTGCG 25 3.887699E-5 45.0 1 GTAGATT 20 7.029538E-4 45.0 8 TATAGCG 20 7.029538E-4 45.0 1 ACCGTAA 20 7.029538E-4 45.0 41 ACTCATA 20 7.029538E-4 45.0 14 TGCGATC 30 2.163084E-6 44.999996 32 CCAACGA 65 0.0 44.999996 37 CGTTTTT 2550 0.0 43.941177 1 CGATCGA 195 0.0 43.846153 41 TGATACC 1620 0.0 43.055553 4 TACGGCT 1935 0.0 42.90698 7 GATACCT 1650 0.0 42.409092 5 TGAATGA 1415 0.0 42.296818 22 GATGAAT 1445 0.0 42.19723 20 AACGATC 80 0.0 42.1875 39 >>END_MODULE