SRR3551935_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548729
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	23000	4.191504367365311	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	18779	3.4222721962936165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	18742	3.4155293414417685	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	6393	1.1650559748072364	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	5424	0.9884660734169326	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	4514	0.8226282919255223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3901	0.7109155885692209	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	3687	0.6719163740206914	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	3516	0.6407534502459319	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	3486	0.635286270636325	RNA PCR Primer, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1405	0.25604624504992446	RNA PCR Primer, Index 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	1396	0.2544060911670424	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATG	1148	0.2092107397276251	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	1072	0.19536055138328756	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGGATA	879	0.16018836256148297	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGGATATCGT	852	0.15526790091283674	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	769	0.14014203732625757	No Hit
TGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCTT	578	0.10533432714509348	RNA PCR Primer, Index 23 (96% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	30	2.1634187E-6	45.000004	12
TATCGAA	120	0.0	45.000004	42
AATCGTT	30	2.1634187E-6	45.000004	22
TTTAGCG	25	3.8881313E-5	45.0	1
TCGATTG	20	7.0300605E-4	45.0	1
CGACAAT	20	7.0300605E-4	45.0	20
CGTTCGG	25	3.8881313E-5	45.0	2
CGTTAGG	45	3.8380676E-10	45.0	2
GCGATCC	80	0.0	45.0	33
CGCAAGT	75	0.0	45.0	23
CCTATCG	95	0.0	45.0	40
CGTCCTA	25	3.8881313E-5	45.0	32
CGATCGA	80	0.0	45.0	41
TCGACAA	20	7.0300605E-4	45.0	19
CCGCAAT	20	7.0300605E-4	45.0	34
GCATCTA	20	7.0300605E-4	45.0	9
CGCTACT	65	0.0	44.999996	42
ACGCTAC	65	0.0	44.999996	41
CCGATGA	1905	0.0	43.70079	18
CGTTTTT	2355	0.0	43.471336	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content