##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548729
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977867034547106	33.0	31.0	34.0	30.0	34.0
2	32.073703048317114	33.0	31.0	34.0	30.0	34.0
3	32.095181774610055	33.0	31.0	34.0	30.0	34.0
4	35.73917908475769	37.0	35.0	37.0	35.0	37.0
5	35.74657982355589	37.0	35.0	37.0	35.0	37.0
6	35.6329408505838	37.0	35.0	37.0	35.0	37.0
7	35.83749537567725	37.0	35.0	37.0	35.0	37.0
8	35.08897105857354	37.0	35.0	37.0	32.0	37.0
9	36.75989240590528	39.0	37.0	39.0	32.0	39.0
10	36.58749218648914	38.0	35.0	39.0	32.0	39.0
11	37.01068651374358	39.0	37.0	39.0	34.0	39.0
12	37.05524949474148	39.0	37.0	39.0	33.0	39.0
13	37.08112201104735	39.0	37.0	39.0	33.0	39.0
14	38.206216547694765	40.0	38.0	41.0	33.0	41.0
15	38.06379834125771	40.0	37.0	41.0	33.0	41.0
16	38.37594878346142	40.0	38.0	41.0	34.0	41.0
17	38.10006214360823	40.0	37.0	41.0	33.0	41.0
18	38.054172460358394	40.0	37.0	41.0	34.0	41.0
19	37.99146937741581	40.0	37.0	41.0	33.0	41.0
20	37.701233213480606	40.0	36.0	41.0	32.0	41.0
21	37.92554065850356	40.0	36.0	41.0	33.0	41.0
22	37.97707429350371	40.0	37.0	41.0	33.0	41.0
23	37.82854924744273	40.0	36.0	41.0	33.0	41.0
24	37.784460088677655	40.0	36.0	41.0	33.0	41.0
25	37.70170339092704	39.0	36.0	41.0	33.0	41.0
26	37.732383380502945	40.0	36.0	41.0	33.0	41.0
27	37.622852081810876	40.0	36.0	41.0	33.0	41.0
28	37.42313054349233	40.0	36.0	41.0	32.0	41.0
29	37.52570030014816	40.0	36.0	41.0	33.0	41.0
30	37.236708830770745	40.0	36.0	41.0	32.0	41.0
31	37.10462723858225	39.0	35.0	41.0	31.0	41.0
32	36.992145485294195	40.0	35.0	41.0	31.0	41.0
33	36.83879109724472	40.0	35.0	41.0	31.0	41.0
34	36.54919277093064	40.0	35.0	41.0	30.0	41.0
35	36.4782032660931	40.0	35.0	41.0	30.0	41.0
36	36.44669955478934	40.0	35.0	41.0	30.0	41.0
37	36.43752380501122	40.0	35.0	41.0	30.0	41.0
38	36.05712655974078	39.0	35.0	41.0	27.0	41.0
39	36.01611360070272	39.0	35.0	41.0	28.0	41.0
40	35.97859234704198	39.0	35.0	41.0	27.0	41.0
41	35.661430323529466	39.0	35.0	41.0	26.0	41.0
42	35.89455086208311	39.0	35.0	41.0	28.0	41.0
43	35.84832039130427	39.0	35.0	41.0	28.0	41.0
44	36.10292694572367	39.0	35.0	41.0	28.0	41.0
45	36.00237275595057	39.0	35.0	41.0	28.0	41.0
46	35.873923193416054	39.0	35.0	41.0	28.0	41.0
47	35.58448523770386	39.0	35.0	41.0	27.0	41.0
48	35.64744527808809	39.0	35.0	41.0	28.0	41.0
49	35.69739707578787	39.0	35.0	41.0	28.0	41.0
50	35.5447880465585	39.0	35.0	41.0	28.0	41.0
51	34.680044247706974	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	7.0
11	5.0
12	5.0
13	10.0
14	11.0
15	14.0
16	22.0
17	57.0
18	120.0
19	356.0
20	1202.0
21	2645.0
22	3860.0
23	4540.0
24	5077.0
25	5875.0
26	6674.0
27	6690.0
28	6427.0
29	6554.0
30	7601.0
31	9644.0
32	12456.0
33	16868.0
34	28155.0
35	36315.0
36	39624.0
37	62708.0
38	116942.0
39	168203.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.85648653524782	15.911679535800003	19.99548046485606	14.236353464096121
2	26.1834165863295	39.80635249822772	21.01583841932903	12.994392496113747
3	23.809020481877212	39.176168928560365	25.239052428430064	11.775758161132362
4	22.34326962854159	20.02901249979498	44.1155834665199	13.512134405143522
5	36.30735754807929	22.866114238540337	23.87881814155986	16.947710071820516
6	19.635557807223602	44.704216471154254	23.946428929398664	11.713796792223484
7	66.54833260133873	3.3269610317661362	26.196173338751915	3.9285330281432183
8	62.981180145390525	17.743184705018326	13.062367762593194	6.213267386997953
9	59.055927425013074	4.5525204609196885	13.196495902348882	23.195056211718352
10	41.44249711606275	17.483311434241674	22.106358512125293	18.967832937570275
11	30.789879886063975	19.048018238511176	27.561510326591087	22.60059154883376
12	21.908082131616883	15.693903548017326	33.092473698310094	29.305540622055698
13	24.154181754563727	17.92287267485407	40.47444184652169	17.44850372406051
14	19.322470654913445	28.167456066655856	29.61789881708457	22.89217446134613
15	14.392532561610558	20.50793743359655	41.5897464868815	23.50978351791139
16	16.473523360347276	21.689030468591962	28.82716240621509	33.010283764845674
17	17.40093926145693	25.95579967524953	33.312436557936614	23.330824505356926
18	16.68783680104387	22.16558629122937	32.252532670954146	28.894044236772615
19	18.00105334327145	23.648832119315728	28.837732286793667	29.512382250619158
20	22.965799146755504	22.85171733223504	35.87289900843586	18.3095845125736
21	21.43918036043293	31.397465780011625	28.255477658370527	18.90787620118492
22	18.737482436685504	19.54352695046189	34.299991434751945	27.418999178100666
23	22.369147612027067	27.390934322771347	29.052410206130897	21.18750785907069
24	22.85554435796176	23.357795924764318	26.395360915861925	27.39129880141199
25	16.817226718471233	34.82374724135229	24.443577795232255	23.915448244944226
26	19.241191918050625	21.044449992619306	32.2151736102885	27.499184479041567
27	24.918493463986778	24.638573867974902	24.717483493673562	25.725449174364762
28	16.904883831545263	26.542610286680674	32.175809917099336	24.37669596467473
29	27.28979149999362	18.360064804302308	28.211922460813994	26.13822123489008
30	20.73209179759043	28.40418494375183	27.522146633401917	23.34157662525582
31	24.228170918613742	26.017761044158412	21.66224128850489	28.09182674872296
32	31.617428639638145	26.336133136757855	22.856091075922723	19.190347147681276
33	26.956840261768562	22.421814775599614	21.87382113939668	28.74752382323515
34	29.32230663952516	25.233767488140774	23.572656083421872	21.871269788912194
35	24.85033595818701	20.51905403213608	26.79428278804291	27.836327221633994
36	26.52766666241442	32.007785263764085	21.500595011380845	19.96395306244066
37	22.48158927266465	23.08115663651821	28.581321563103096	25.855932527714042
38	23.92036870659287	27.80789788766404	22.655810062890787	25.615923342852298
39	28.626516914542517	27.818285528922292	23.57302056206251	19.98217699447268
40	25.265841608517135	20.867495612588364	30.252091651798974	23.614571127095523
41	19.53113467668011	32.997162533782614	23.143300244747408	24.328402544789867
42	21.255665364870456	21.257669997393975	34.76105691516213	22.725607722573436
43	25.707771960293694	26.50616242261663	23.66614485474615	24.119920762343526
44	23.180294826772414	21.379041384727252	29.321395442923553	26.119268345576778
45	21.15816732849913	20.06035766289006	27.66629793577522	31.115177072835586
46	30.854028126816697	25.018542850842586	23.76127377995331	20.366155242387407
47	19.343610416070593	22.49179467460258	36.79065622556854	21.373938683758283
48	22.848072545828632	26.715919880305215	24.867648693617433	25.56835888024872
49	21.065589753776454	19.501611906788234	36.28767570148471	23.14512263795061
50	24.475469676288295	20.609991452975876	29.40959927395855	25.50493959677728
51	22.099615657273443	19.542251275219645	26.38843582168976	31.96969724581715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	165.5
2	269.0
3	3562.5
4	6856.0
5	4594.5
6	2333.0
7	2240.5
8	2148.0
9	2183.5
10	2219.0
11	2132.5
12	2046.0
13	1930.5
14	1815.0
15	1648.5
16	1482.0
17	1447.0
18	1412.0
19	1433.5
20	1455.0
21	1359.5
22	1264.0
23	1336.5
24	1409.0
25	1666.0
26	2286.0
27	2649.0
28	3201.5
29	3754.0
30	4349.5
31	4945.0
32	5949.5
33	6954.0
34	8102.0
35	9250.0
36	10196.5
37	11143.0
38	11996.0
39	12849.0
40	14029.5
41	15210.0
42	16966.5
43	18723.0
44	22164.5
45	25606.0
46	69945.5
47	114285.0
48	83203.0
49	52121.0
50	52005.5
51	51890.0
52	44473.5
53	37057.0
54	34580.0
55	32103.0
56	28618.5
57	25134.0
58	23870.5
59	22607.0
60	20979.5
61	19352.0
62	17850.0
63	16348.0
64	14747.5
65	13147.0
66	11342.0
67	9537.0
68	7995.5
69	6454.0
70	5702.0
71	4950.0
72	3866.5
73	2783.0
74	2157.0
75	1202.0
76	873.0
77	684.0
78	495.0
79	317.0
80	139.0
81	117.0
82	95.0
83	62.5
84	30.0
85	23.0
86	16.0
87	8.5
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	548729.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.700425701959205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94478977523936	22.394360988397693
2	9.761646445648061	5.99373402866825
3	3.4390132370467925	3.167375111160278
4	1.9433178601931747	2.386427423286036
5	1.3444725350041282	2.063793958460949
6	1.104133256049312	2.033841659544252
7	0.9284977398477489	1.9953693113642998
8	0.8770937270788686	2.1541720641471427
9	0.8153601941424224	2.252871455454403
>10	6.737903371729777	34.25137434060609
>50	0.06142816882816898	1.3288981779184799
>100	0.031608669397018986	1.7523952136756367
>500	0.002385559954491999	0.5635641487507689
>1k	0.005367509897606998	4.417149151595939
>5k	0.0011927799772459995	2.163624933654268
>10k+	0.0017891699658689995	11.081048033315557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	23000	4.191504367365311	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	18779	3.4222721962936165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	18742	3.4155293414417685	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	6393	1.1650559748072364	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	5424	0.9884660734169326	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	4514	0.8226282919255223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3901	0.7109155885692209	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	3687	0.6719163740206914	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	3516	0.6407534502459319	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	3486	0.635286270636325	RNA PCR Primer, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1405	0.25604624504992446	RNA PCR Primer, Index 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	1396	0.2544060911670424	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATG	1148	0.2092107397276251	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	1072	0.19536055138328756	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGGATA	879	0.16018836256148297	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGGATATCGT	852	0.15526790091283674	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	769	0.14014203732625757	No Hit
TGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCTT	578	0.10533432714509348	RNA PCR Primer, Index 23 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2026501241960968	0.0
2	0.0	0.0	0.0	1.8404348959140122	0.0
3	0.0	0.0	0.0	2.156073398708652	0.0
4	0.0	0.0	0.0	3.14891321581327	0.0
5	0.0	0.0	0.0	7.5948236743456246	0.0
6	0.0	0.0	0.0	8.580738397278074	0.0
7	0.0	0.0	0.0	10.063255268083152	0.0
8	0.0	0.0	0.0	11.752613767451694	0.0
9	0.0	0.0	0.0	12.12164839110016	0.0
10	0.0	0.0	0.0	16.39224462348445	0.0
11	0.0	0.0	0.0	17.669742258929272	0.0
12	0.0	0.0	0.0	22.983658600146885	0.0
13	0.0	0.0	0.0	23.54932945042088	0.0
14	0.0	0.0	0.0	23.837632055167486	0.0
15	0.0	0.0	0.0	24.804411649466314	0.0
16	0.0	0.0	0.0	25.556513324427904	0.0
17	0.0	0.0	0.0	26.34888988918027	0.0
18	0.0	0.0	0.0	27.02882479329505	0.0
19	0.0	0.0	0.0	28.607564025229212	0.0
20	0.0	0.0	0.0	29.23811207353721	0.0
21	0.0	0.0	0.0	29.871575950970332	0.0
22	0.0	0.0	0.0	30.590874548274286	0.0
23	0.0	0.0	0.0	31.19572685241713	0.0
24	0.0	0.0	0.0	31.677567615343822	0.0
25	0.0	0.0	0.0	32.11658213799526	0.0
26	0.0	0.0	0.0	32.551222916959006	0.0
27	0.0	0.0	0.0	33.03142352600282	0.0
28	0.0	0.0	0.0	33.4150372952769	0.0
29	0.0	0.0	0.0	33.8230711334739	0.0
30	0.0	0.0	0.0	34.26645939981302	0.0
31	0.0	0.0	0.0	34.68943686227628	0.0
32	0.0	0.0	0.0	35.13300736793572	0.0
33	0.0	0.0	0.0	35.588423429415975	0.0
34	1.8223932032023093E-4	0.0	0.0	36.01322328508244	0.0
35	1.8223932032023093E-4	0.0	0.0	36.476840115977105	0.0
36	1.8223932032023093E-4	0.0	0.0	36.89435039883075	0.0
37	1.8223932032023093E-4	0.0	0.0	37.30675798071543	0.0
38	1.8223932032023093E-4	0.0	0.0	37.72117019512364	0.0
39	1.8223932032023093E-4	0.0	0.0	38.12628820419551	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	30	2.1634187E-6	45.000004	12
TATCGAA	120	0.0	45.000004	42
AATCGTT	30	2.1634187E-6	45.000004	22
TTTAGCG	25	3.8881313E-5	45.0	1
TCGATTG	20	7.0300605E-4	45.0	1
CGACAAT	20	7.0300605E-4	45.0	20
CGTTCGG	25	3.8881313E-5	45.0	2
CGTTAGG	45	3.8380676E-10	45.0	2
GCGATCC	80	0.0	45.0	33
CGCAAGT	75	0.0	45.0	23
CCTATCG	95	0.0	45.0	40
CGTCCTA	25	3.8881313E-5	45.0	32
CGATCGA	80	0.0	45.0	41
TCGACAA	20	7.0300605E-4	45.0	19
CCGCAAT	20	7.0300605E-4	45.0	34
GCATCTA	20	7.0300605E-4	45.0	9
CGCTACT	65	0.0	44.999996	42
ACGCTAC	65	0.0	44.999996	41
CCGATGA	1905	0.0	43.70079	18
CGTTTTT	2355	0.0	43.471336	1
>>END_MODULE