SRR3551934_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505216
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	20452	4.04816949581961	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	16662	3.2979953128958703	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	15809	3.1291566379528755	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	6063	1.2000807575373702	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGGACT	4731	0.936431150240689	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.8303379148720548	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	3926	0.7770933620471243	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTA	3291	0.6514045477577908	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	3164	0.626266784899924	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	2911	0.5761891943248036	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTT	1167	0.23099030909551557	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATG	1124	0.22247909804915125	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1102	0.21812452495566254	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	1060	0.20981124904991133	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTACGGAC	890	0.17616227514568025	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTACGGACTCGT	788	0.15597289080314164	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTAT	687	0.1359814416012161	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	577	0.11420857613377247	TruSeq Adapter, Index 21 (95% over 23bp)
TGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCTT	560	0.11084367874334938	TruSeq Adapter, Index 21 (96% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTCGA	145	0.0	45.000004	41
GACGCTA	105	0.0	45.000004	40
GATACCC	85	0.0	45.0	35
ACGTTCG	40	6.8012014E-9	45.0	13
CAGCACG	20	7.029528E-4	45.0	1
CAACCCG	20	7.029528E-4	45.0	25
CGTTCGA	40	6.8012014E-9	45.0	14
ATCGTGT	45	3.8380676E-10	45.0	13
TCGCTAA	20	7.029528E-4	45.0	11
CGAATGT	25	3.887691E-5	45.0	45
CGTAAGC	50	2.1827873E-11	45.0	43
ATACGAG	20	7.029528E-4	45.0	1
TTACACG	45	3.8380676E-10	45.0	34
GTAACGG	20	7.029528E-4	45.0	2
TTGTACG	30	2.1630767E-6	44.999996	1
CGTTTTT	2520	0.0	43.839287	1
GATACCT	1615	0.0	43.746128	5
TGATACC	1625	0.0	43.615383	4
TACGGCT	2320	0.0	43.254314	7
GATGAAT	1880	0.0	42.965427	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content