SRR3551933_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	29740	4.230049853143024	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	25193	3.583310220249906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	24213	3.443920547886753	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	8713	1.2392879747960714	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	6568	0.9341952735522321	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	5823	0.8282306756843251	No Hit
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	5226	0.7433167630304452	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5089	0.7238306557715147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	4891	0.6956682525797757	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	4724	0.671915114534218	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT	2188	0.3112087787046717	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG	1611	0.22913955324187665	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1491	0.21207143009536814	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1458	0.20737769623007832	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACACCTTGTCGT	1213	0.17253027813929012	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACACCTTG	1155	0.16428068528514433	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTAT	1125	0.1600136544985172	No Hit
TGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCTT	851	0.12104143998065613	TruSeq Adapter, Index 20 (96% over 25bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCTGTCGAATGC	731	0.10397331683414764	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	718	0.10212427015994253	TruSeq Adapter, Index 20 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	95	0.0	45.000004	41
ACGGACG	30	2.1642936E-6	45.000004	43
TTAACGA	30	2.1642936E-6	45.000004	44
CTCGAAT	30	2.1642936E-6	45.000004	43
ACGCCTA	20	7.031416E-4	45.0	30
ATTAGCG	45	3.8380676E-10	45.0	1
GATCGAT	20	7.031416E-4	45.0	9
CGACGTA	20	7.031416E-4	45.0	45
ACGCTTA	20	7.031416E-4	45.0	30
ATAACGG	25	3.8892547E-5	45.0	2
ATACGAT	20	7.031416E-4	45.0	14
CCTATCG	25	3.8892547E-5	45.0	40
TGTACGG	85	0.0	45.0	2
CGTTTTT	3430	0.0	43.622448	1
GTCGAAT	305	0.0	43.52459	43
ACGGCTG	3370	0.0	43.063797	8
CCGATGA	2115	0.0	42.87234	18
CGATGAA	2165	0.0	42.817554	19
TACGGCT	3410	0.0	42.49267	7
CGAATGC	345	0.0	42.391304	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content