##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977256725907278	33.0	31.0	34.0	30.0	34.0
2	32.07312979596481	33.0	31.0	34.0	30.0	34.0
3	32.091311614146626	33.0	31.0	34.0	30.0	34.0
4	35.749781314672184	37.0	35.0	37.0	35.0	37.0
5	35.747992006428994	37.0	35.0	37.0	35.0	37.0
6	35.64267884192785	37.0	35.0	37.0	35.0	37.0
7	35.834790524346964	37.0	35.0	37.0	35.0	37.0
8	35.07791313747662	37.0	35.0	37.0	32.0	37.0
9	36.817046787992574	39.0	37.0	39.0	32.0	39.0
10	36.60142518828273	38.0	35.0	39.0	32.0	39.0
11	36.999977242502474	39.0	37.0	39.0	34.0	39.0
12	37.07758742079324	39.0	37.0	39.0	34.0	39.0
13	37.13038481505978	39.0	37.0	39.0	33.0	39.0
14	38.2489471101534	40.0	38.0	41.0	33.0	41.0
15	38.119937701350516	40.0	37.0	41.0	33.0	41.0
16	38.40735778341974	40.0	38.0	41.0	34.0	41.0
17	38.15725430792317	40.0	37.0	41.0	33.0	41.0
18	38.072666111952664	40.0	37.0	41.0	34.0	41.0
19	38.00959939692631	40.0	37.0	41.0	34.0	41.0
20	37.77353871974853	40.0	36.0	41.0	33.0	41.0
21	37.951254862637164	40.0	36.0	41.0	33.0	41.0
22	38.02005788938434	40.0	37.0	41.0	34.0	41.0
23	37.90503154046923	40.0	36.0	41.0	33.0	41.0
24	37.791993627900695	40.0	36.0	41.0	33.0	41.0
25	37.73943376501462	39.0	36.0	41.0	33.0	41.0
26	37.71733196788348	40.0	36.0	41.0	33.0	41.0
27	37.68965173917063	40.0	36.0	41.0	33.0	41.0
28	37.529164444254796	40.0	36.0	41.0	33.0	41.0
29	37.57771187585785	40.0	36.0	41.0	33.0	41.0
30	37.32691287434306	40.0	36.0	41.0	32.0	41.0
31	37.21353502165518	40.0	36.0	41.0	31.0	41.0
32	37.012711484713364	40.0	36.0	41.0	31.0	41.0
33	36.851080625546714	40.0	36.0	41.0	30.0	41.0
34	36.65508736745536	40.0	36.0	41.0	30.0	41.0
35	36.61648638461593	40.0	36.0	41.0	30.0	41.0
36	36.54478319927745	40.0	35.0	41.0	30.0	41.0
37	36.63023475781044	40.0	36.0	41.0	30.0	41.0
38	36.31016335616195	40.0	35.0	41.0	29.0	41.0
39	36.239930874101255	39.0	35.0	41.0	29.0	41.0
40	36.300561114548444	39.0	35.0	41.0	29.0	41.0
41	35.95415359888488	39.0	35.0	41.0	26.0	41.0
42	36.0831829204981	39.0	35.0	41.0	29.0	41.0
43	36.02535754162133	39.0	35.0	41.0	29.0	41.0
44	36.206438949457024	39.0	35.0	41.0	29.0	41.0
45	36.221101889583466	39.0	35.0	41.0	29.0	41.0
46	36.14303656134212	39.0	35.0	41.0	29.0	41.0
47	35.877123736781094	39.0	35.0	41.0	28.0	41.0
48	35.95829119640432	39.0	35.0	41.0	28.0	41.0
49	36.01411960487295	39.0	35.0	41.0	29.0	41.0
50	35.81910776386252	39.0	35.0	41.0	28.0	41.0
51	35.11182038645075	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	6.0
12	4.0
13	6.0
14	16.0
15	28.0
16	31.0
17	83.0
18	178.0
19	480.0
20	1096.0
21	2245.0
22	3909.0
23	5332.0
24	6647.0
25	7993.0
26	8891.0
27	9003.0
28	8677.0
29	8275.0
30	9286.0
31	11863.0
32	15386.0
33	21206.0
34	34830.0
35	43849.0
36	49608.0
37	77500.0
38	148894.0
39	227664.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.719869428857926	16.171904446957253	18.990562750243576	15.117663373941243
2	26.64618491889086	39.27688051602626	20.28631776578268	13.790616799300206
3	23.626549465554394	38.84235454758806	25.29254051901318	12.238555467844368
4	22.545852801661297	19.612553604574256	43.773619793333474	14.06797380043097
5	35.23073968978686	22.368628789656718	24.60313057825379	17.797500942302634
6	19.64925006933925	42.92462290115423	25.511581432726704	11.914545596779815
7	65.42794762930882	3.508210478405269	26.782445435343817	4.281396456942104
8	61.98559165937716	17.424989154630083	14.020183055620747	6.569236130372014
9	58.22491519276311	4.883190032216082	14.109363999061253	22.782530775959547
10	40.602646981431306	19.098660863504797	22.80983977299396	17.488852382069936
11	29.67293209020503	19.105914815842063	29.10555922994318	22.11559386400973
12	21.03247921600421	16.044889163875318	34.17550297625397	28.7471286438665
13	23.372803368109636	18.148819810401598	41.26161876924609	17.21675805224268
14	19.491227695874493	28.301366161023516	29.903636221402003	22.303769921699985
15	14.909290037194284	20.3608485701891	42.42737158015262	22.302489812463996
16	15.974056452817306	22.5878119377298	28.709863241663285	32.72826836778961
17	17.581731418858855	25.584263190458916	33.95489748458535	22.879107906096877
18	17.206517178354773	22.56377433096513	32.38406121766835	27.845647273011743
19	17.4137526402253	24.226636228513723	29.686871057441344	28.67274007381963
20	21.857438501418788	23.62413148144197	36.36064944208572	18.15778057505352
21	20.768207775952437	31.34304794009089	29.55686885280877	18.331875431147903
22	17.94727372291324	19.83955964242282	34.94328404912775	27.269882585536187
23	21.06433971254436	27.90225654811433	30.441993272314793	20.59141046702652
24	22.274896346710474	23.314771749411506	27.483234124867543	26.927097779010477
25	16.421667982334494	34.65753522078329	25.460946000725393	23.45985079615683
26	17.65341753607419	22.5962037649435	32.64918606387745	27.101192635104866
27	24.328333795595004	25.15841351795353	25.353559059261947	25.159693627189522
28	15.407110295634116	27.245702744411965	33.09082375029336	24.25636320966056
29	20.374076365627644	22.825770021264034	28.57829645907562	28.221857154032698
30	17.252458876490795	28.801035466137552	28.537475197883555	25.409030459488097
31	21.250808957919965	25.516844104030213	24.695725146323596	28.53662179172623
32	23.227866555723867	27.35892129461714	24.44041447092374	24.97279767873525
33	21.42490381401435	19.916935134020324	24.040024748778563	34.61813630318676
34	22.03878730985044	25.006507221949608	29.081521623178514	23.873183845021444
35	20.443913436168774	20.22117442910684	28.515286637793093	30.81962549693129
36	22.80002560218472	31.38074004537276	23.907746794393123	21.911487558049398
37	17.54531942281297	20.84985029833657	31.012210819767734	30.59261945908273
38	18.844914766060036	26.57037400524845	23.507783775326605	31.07692745336491
39	22.69733239458656	21.585486405951084	28.943127591332242	26.774053608130117
40	18.70438721882045	19.378435848747984	32.00301536842255	29.914161564009017
41	21.941925710994003	23.852417628526524	29.2674219311159	24.93823472936357
42	20.080646881867253	20.239807130208444	36.17745158697986	23.502094400944436
43	27.763578047548947	21.794570914495814	24.741240141380956	25.700610896574283
44	21.452497279767872	20.214347179848236	30.870118694573044	27.46303684581084
45	19.276738281666702	19.60117485580992	28.95223059034371	32.16985627217967
46	28.52766102707431	23.56581539402473	25.31615142269918	22.590372156201774
47	17.005682262664195	21.407266753429628	37.94172658289063	23.645324401015554
48	21.75474529382063	23.967342991046348	27.08597355863256	27.191938156500466
49	19.645978679069504	18.04285521253369	37.59581262045472	24.71535348794208
50	23.785709713895585	18.86411640459986	30.142447711093567	27.207726170410986
51	20.37052050663879	18.578652044974504	27.723894661233313	33.32693278715339
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	189.0
2	339.0
3	4745.5
4	9152.0
5	6225.0
6	3298.0
7	3253.0
8	3208.0
9	3149.0
10	3090.0
11	3034.5
12	2979.0
13	2799.0
14	2619.0
15	2454.0
16	2289.0
17	2227.0
18	2165.0
19	2062.5
20	1960.0
21	1947.5
22	1935.0
23	1935.5
24	1936.0
25	2145.0
26	3019.0
27	3684.0
28	4306.5
29	4929.0
30	5971.5
31	7014.0
32	7907.0
33	8800.0
34	10234.5
35	11669.0
36	13016.0
37	14363.0
38	15847.0
39	17331.0
40	18812.5
41	20294.0
42	23524.0
43	26754.0
44	31261.5
45	35769.0
46	94506.5
47	153244.0
48	112221.5
49	71199.0
50	70123.5
51	69048.0
52	57530.5
53	46013.0
54	42225.5
55	38438.0
56	34155.0
57	29872.0
58	27102.0
59	24332.0
60	22406.0
61	20480.0
62	18941.5
63	17403.0
64	15554.0
65	13705.0
66	12380.0
67	11055.0
68	9131.0
69	7207.0
70	6314.0
71	5421.0
72	4488.5
73	3556.0
74	2766.0
75	1508.5
76	1041.0
77	790.0
78	539.0
79	404.0
80	269.0
81	219.0
82	169.0
83	113.5
84	58.0
85	48.0
86	38.0
87	22.0
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	10.5
94	20.0
95	10.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.409506505143465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03297525850077	22.939297114718617
2	10.226324718304408	6.424076255265835
3	3.6395334737606366	3.4294785095931632
4	1.9887849286135466	2.498670126104739
5	1.3204580458623607	2.0737467790641424
6	1.0362681044637985	1.952920186093702
7	0.9303603273223509	2.045551112721115
8	0.7905403721892628	1.9864386370285747
9	0.7484535217752651	2.1157700184897954
>10	6.173698511873612	32.02542069525211
>50	0.06519105845882486	1.345987298058104
>100	0.03692640373925741	1.922184413605409
>500	0.002735289165870919	0.5654578552836531
>1k	0.0041029337488063785	2.843183381745307
>5k	0.002279407638225766	4.498890948381134
>10k+	0.0013676445829354596	11.332926668594585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	29740	4.230049853143024	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	25193	3.583310220249906	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	24213	3.443920547886753	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	8713	1.2392879747960714	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	6568	0.9341952735522321	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	5823	0.8282306756843251	No Hit
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	5226	0.7433167630304452	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5089	0.7238306557715147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	4891	0.6956682525797757	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	4724	0.671915114534218	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTT	2188	0.3112087787046717	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATG	1611	0.22913955324187665	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1491	0.21207143009536814	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1458	0.20737769623007832	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACACCTTGTCGT	1213	0.17253027813929012	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACACCTTG	1155	0.16428068528514433	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTAT	1125	0.1600136544985172	No Hit
TGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCTT	851	0.12104143998065613	TruSeq Adapter, Index 20 (96% over 25bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCTGTCGAATGC	731	0.10397331683414764	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	718	0.10212427015994253	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8446871910847504E-4	0.0	0.0	0.21320930497180204	0.0
2	2.8446871910847504E-4	0.0	0.0	1.8848897328127556	0.0
3	2.8446871910847504E-4	0.0	0.0	2.2477295840356155	0.0
4	2.8446871910847504E-4	0.0	0.0	3.3934273502449988	0.0
5	2.8446871910847504E-4	0.0	0.0	8.119021712074986	0.0
6	2.8446871910847504E-4	0.0	0.0	9.187912924125081	0.0
7	2.8446871910847504E-4	0.0	0.0	10.765291971581576	0.0
8	2.8446871910847504E-4	0.0	0.0	12.58006016513409	0.0
9	2.8446871910847504E-4	0.0	0.0	12.991970870403163	0.0
10	2.8446871910847504E-4	0.0	0.0	17.362548270785773	0.0
11	2.8446871910847504E-4	0.0	0.0	18.724726732236707	0.0
12	2.8446871910847504E-4	0.0	0.0	24.20416319970415	0.0
13	2.8446871910847504E-4	0.0	0.0	24.84891155156351	0.0
14	2.8446871910847504E-4	0.0	0.0	25.194825513999415	0.0
15	2.8446871910847504E-4	0.0	0.0	26.21564151252018	0.0
16	2.8446871910847504E-4	0.0	0.0	27.00404656752932	0.0
17	2.8446871910847504E-4	0.0	0.0	27.87722330083278	0.0
18	2.8446871910847504E-4	0.0	0.0	28.6464267173021	0.0
19	4.2670307866271253E-4	0.0	0.0	30.217547452938206	0.0
20	4.2670307866271253E-4	0.0	0.0	30.899561207000776	0.0
21	4.2670307866271253E-4	0.0	0.0	31.59963872472673	0.0
22	4.2670307866271253E-4	0.0	0.0	32.365428516566745	0.0
23	4.2670307866271253E-4	0.0	0.0	33.048437911146195	0.0
24	4.2670307866271253E-4	0.0	0.0	33.59604019543001	0.0
25	4.2670307866271253E-4	0.0	0.0	34.08091712715041	0.0
26	4.2670307866271253E-4	0.0	0.0	34.60149488311892	0.0
27	4.2670307866271253E-4	0.0	0.0	35.10315546926671	0.0
28	4.2670307866271253E-4	0.0	0.0	35.56356809114378	0.0
29	4.2670307866271253E-4	0.0	0.0	36.022700603784855	0.0
30	4.2670307866271253E-4	0.0	0.0	36.539580266404954	0.0
31	4.2670307866271253E-4	0.0	0.0	37.022608151451145	0.0
32	4.2670307866271253E-4	0.0	0.0	37.48259407024955	0.0
33	4.2670307866271253E-4	0.0	0.0	37.9626350337451	0.0
34	4.2670307866271253E-4	0.0	0.0	38.4257501084537	0.0
35	4.2670307866271253E-4	0.0	0.0	38.90095510372441	0.0
36	4.2670307866271253E-4	0.0	0.0	39.36947508409607	0.0
37	5.689374382169501E-4	0.0	0.0	39.83358579932154	0.0
38	5.689374382169501E-4	0.0	0.0	40.30594610740116	0.0
39	5.689374382169501E-4	0.0	0.0	40.75597562103077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGA	95	0.0	45.000004	41
ACGGACG	30	2.1642936E-6	45.000004	43
TTAACGA	30	2.1642936E-6	45.000004	44
CTCGAAT	30	2.1642936E-6	45.000004	43
ACGCCTA	20	7.031416E-4	45.0	30
ATTAGCG	45	3.8380676E-10	45.0	1
GATCGAT	20	7.031416E-4	45.0	9
CGACGTA	20	7.031416E-4	45.0	45
ACGCTTA	20	7.031416E-4	45.0	30
ATAACGG	25	3.8892547E-5	45.0	2
ATACGAT	20	7.031416E-4	45.0	14
CCTATCG	25	3.8892547E-5	45.0	40
TGTACGG	85	0.0	45.0	2
CGTTTTT	3430	0.0	43.622448	1
GTCGAAT	305	0.0	43.52459	43
ACGGCTG	3370	0.0	43.063797	8
CCGATGA	2115	0.0	42.87234	18
CGATGAA	2165	0.0	42.817554	19
TACGGCT	3410	0.0	42.49267	7
CGAATGC	345	0.0	42.391304	45
>>END_MODULE