SRR3551931_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439251
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	16702	3.802381781714783	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	15173	3.454289233262986	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	14332	3.262826948601141	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	4245	0.9664178339946865	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3333	0.758791670366146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	3201	0.7287405151041204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	3055	0.695502116102183	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	2749	0.6258380743583964	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	2510	0.5714272705127592	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	2507	0.5707442897113496	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	994	0.22629430553373814	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	878	0.19988571454589743	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	873	0.19874741321021464	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	663	0.1509387571115376	Illumina Paired End PCR Primer 2 (95% over 21bp)
TAGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	587	0.13363657680915922	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	574	0.13067699333638397	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	530	0.12065994158237546	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	518	0.11792801837673676	No Hit
TAGTGAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	506	0.11519609517109806	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	466	0.10608968448563579	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	20	7.028521E-4	45.000004	45
TAAAGCG	40	6.7993824E-9	45.000004	1
GGGTCGA	20	7.028521E-4	45.000004	8
GCGTTAG	20	7.028521E-4	45.000004	1
GTTGCGC	20	7.028521E-4	45.000004	32
AATGCGG	25	3.8868573E-5	45.0	2
CGATCGA	85	0.0	45.0	41
ACGGGTA	60	0.0	44.999996	5
TGTTGCG	30	2.1624273E-6	44.999996	1
TACGAAT	30	2.1624273E-6	44.999996	12
GGTATTC	30	2.1624273E-6	44.999996	8
CGTTTTT	1840	0.0	43.28804	1
GATGAAT	1515	0.0	42.92079	20
TACGGCT	1830	0.0	42.663937	7
CATCGAA	95	0.0	42.63158	42
ATGAATG	1515	0.0	42.62376	21
CCGATGA	1425	0.0	42.473686	18
CCGATCG	85	0.0	42.35294	40
ACGGCTG	1850	0.0	42.324326	8
CGATGAA	1465	0.0	41.928326	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content