##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	439251
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91459097418105	33.0	31.0	34.0	30.0	34.0
2	32.00931358152856	33.0	31.0	34.0	30.0	34.0
3	32.03382803909382	33.0	31.0	34.0	30.0	34.0
4	35.684975105349785	37.0	35.0	37.0	35.0	37.0
5	35.687404240400134	37.0	35.0	37.0	35.0	37.0
6	35.57132482339255	37.0	35.0	37.0	33.0	37.0
7	35.74017589032239	37.0	35.0	37.0	35.0	37.0
8	34.930162936453186	37.0	35.0	37.0	32.0	37.0
9	36.638901220486694	39.0	35.0	39.0	32.0	39.0
10	36.4869493751864	38.0	35.0	39.0	32.0	39.0
11	36.89478453094017	39.0	37.0	39.0	33.0	39.0
12	36.91130583652627	39.0	37.0	39.0	33.0	39.0
13	36.951465107649156	39.0	37.0	39.0	33.0	39.0
14	38.05630038406287	40.0	37.0	41.0	33.0	41.0
15	37.94003200903356	40.0	37.0	41.0	33.0	41.0
16	38.21803479104202	40.0	37.0	41.0	34.0	41.0
17	37.95792838263316	40.0	37.0	41.0	33.0	41.0
18	37.875522195737744	40.0	37.0	41.0	33.0	41.0
19	37.826110811358426	40.0	37.0	41.0	33.0	41.0
20	37.62546015831495	40.0	36.0	41.0	32.0	41.0
21	37.78917976282354	40.0	36.0	41.0	33.0	41.0
22	37.809837655463504	40.0	36.0	41.0	33.0	41.0
23	37.730534478009154	40.0	36.0	41.0	33.0	41.0
24	37.60042891194328	40.0	35.0	41.0	33.0	41.0
25	37.567104002039834	39.0	36.0	41.0	33.0	41.0
26	37.538464340434054	39.0	36.0	41.0	33.0	41.0
27	37.45147535236118	40.0	36.0	41.0	32.0	41.0
28	37.27319459716654	40.0	35.0	41.0	32.0	41.0
29	37.380288263430245	40.0	35.0	41.0	32.0	41.0
30	37.06996910650175	39.0	35.0	41.0	31.0	41.0
31	37.15417608611022	39.0	35.0	41.0	31.0	41.0
32	36.82222692720108	39.0	35.0	41.0	31.0	41.0
33	36.67826140407193	40.0	35.0	41.0	30.0	41.0
34	36.581792642475484	40.0	35.0	41.0	30.0	41.0
35	36.39076518892387	40.0	35.0	41.0	30.0	41.0
36	36.32382851718038	40.0	35.0	41.0	30.0	41.0
37	36.33540048855893	40.0	35.0	41.0	30.0	41.0
38	36.290209925532324	39.0	35.0	41.0	30.0	41.0
39	35.93131262080223	39.0	35.0	41.0	28.0	41.0
40	35.85745735354046	39.0	35.0	41.0	27.0	41.0
41	35.67965923811215	39.0	35.0	41.0	26.0	41.0
42	35.721737685287	39.0	35.0	41.0	27.0	41.0
43	35.739506569137006	39.0	35.0	41.0	27.0	41.0
44	35.99779852521679	39.0	35.0	41.0	29.0	41.0
45	35.95911449262495	39.0	35.0	41.0	28.0	41.0
46	35.81663331443753	39.0	35.0	41.0	28.0	41.0
47	35.58227528224182	39.0	35.0	41.0	27.0	41.0
48	35.56997935121377	38.0	35.0	40.0	27.0	41.0
49	35.57835041923638	39.0	35.0	41.0	27.0	41.0
50	35.45427557364696	38.0	35.0	40.0	27.0	41.0
51	34.59281595261024	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	8.0
12	6.0
13	9.0
14	14.0
15	15.0
16	33.0
17	73.0
18	119.0
19	323.0
20	791.0
21	1795.0
22	3027.0
23	3881.0
24	4352.0
25	5077.0
26	5604.0
27	5566.0
28	5386.0
29	5539.0
30	6184.0
31	7864.0
32	10547.0
33	14405.0
34	24471.0
35	30147.0
36	32706.0
37	50476.0
38	90941.0
39	129848.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.017416010435944	15.091371448215257	19.24503302212175	15.646179519227049
2	26.68246628920594	39.2859663381529	20.08874197212983	13.942825400511325
3	25.764540092111343	37.562122795394885	24.129711713803726	12.543625398690041
4	23.247528178649564	19.53780412565936	42.55448479343246	14.660182902258617
5	35.75017472925503	22.483500322139278	23.49636085062982	18.269964097975873
6	20.333932079835904	43.07810340784654	24.131760656207955	12.456203856109605
7	65.06097880255253	5.016493986354044	25.374785714773555	4.547741496319872
8	61.927462885684946	17.70809855868285	14.042540597517137	6.321897958115064
9	58.18290681182285	5.766634566569	13.333378865386761	22.717079756221388
10	42.291537184889734	18.706161169809516	21.804617405538064	17.197684239762687
11	32.646937627916614	19.392101554691966	26.67996202626744	21.28099879112398
12	23.858568335644083	16.916751470116175	30.858666229558956	28.366013964680786
13	25.278485421774793	18.556588374300798	37.7422020666999	18.422724137224503
14	20.446396251801364	28.265615786873568	27.70443322838195	23.583554732943124
15	16.16387896669558	20.893976336991834	39.33696223799149	23.605182458321096
16	17.81896910877835	22.458685353021394	27.284627695782138	32.43771784241812
17	18.55727135510221	26.13858591101671	32.44932851604208	22.854814217839003
18	17.981290879246718	23.389360525075638	29.900899485715453	28.728449109962185
19	19.08999638020175	24.610530197996134	27.21086576922989	29.088607652572218
20	24.04820933816884	23.49590553009555	33.74471543604909	18.71116969568652
21	22.001315876344048	31.73606889910325	27.6723331307157	18.59028209383701
22	19.424656972892492	20.271553166640484	32.94744918053687	27.356340679930153
23	23.58833559855299	26.944275596412986	28.64808503566298	20.819303769371043
24	23.499320434102597	23.62271229889061	25.879280866748168	26.99868640025862
25	18.763759217395066	34.171806097197276	23.966251641999676	23.098183043407982
26	19.041732403568805	20.82818251978937	31.33789109188141	28.792193984760424
27	25.643880150528968	24.75350084575789	24.4290849650883	25.17353403862484
28	17.217035362469293	25.44923062212721	31.078130727078594	26.255603288324895
29	21.649808423885204	19.040821762500258	28.706366064049938	30.6030037495646
30	18.694322835918413	27.13846980428047	28.06140452725207	26.105802832549042
31	23.178091797172915	20.337119323575813	22.468474744508267	34.016314134743006
32	25.2648258057466	24.63124728230556	28.117181292700526	21.986745619247312
33	23.058114836391947	20.28452980186727	26.71524936767361	29.942105994067177
34	26.700679110576868	19.558065889434513	25.631131175569323	28.110123824419297
35	22.539960068389146	19.67872583101689	28.980469025682353	28.800845074911614
36	20.19141675260842	27.444445203312	22.678149850541036	29.68598819353855
37	19.75681330264473	21.111847212641518	31.986495192953456	27.14484429176029
38	19.203371193235757	22.129260946474794	27.231355193272183	31.436012667017266
39	25.393681516945893	22.822941780439884	29.760433100892204	22.022943601722023
40	20.216687042260574	19.378897259198045	35.44351634942208	24.9608993491193
41	22.877807904819797	26.87779879840911	23.84035551427316	26.404037782497937
42	19.833990133204022	20.604847797728407	35.622912639925694	23.93824942914188
43	22.82658434471407	25.588786365881923	25.22475759873057	26.359871690673444
44	22.809737484945966	19.708094005477506	30.086442603431752	27.39572590614478
45	20.674284179205056	18.053231523661868	28.307732936293828	32.964751360839244
46	27.571251972107063	23.988107027644787	25.810072145538655	22.630568854709495
47	17.882941643843726	22.027496807064754	37.387052049966876	22.702509499124645
48	22.827722646049754	23.408483987515112	26.173417931888604	27.590375434546534
49	19.747706891959265	19.119592214929504	36.793314073274736	24.339386819836495
50	23.045365861432302	19.74474730848649	30.35439873785148	26.855488092229727
51	20.583903053151843	18.6742887324104	27.714677940403092	33.02713027403467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	216.0
2	365.0
3	3019.5
4	5674.0
5	3799.5
6	1925.0
7	1888.0
8	1851.0
9	1783.5
10	1716.0
11	1657.5
12	1599.0
13	1492.5
14	1386.0
15	1286.5
16	1187.0
17	1093.0
18	999.0
19	988.5
20	978.0
21	1036.5
22	1095.0
23	1068.0
24	1041.0
25	1332.5
26	1894.5
27	2165.0
28	2648.5
29	3132.0
30	3832.0
31	4532.0
32	5042.0
33	5552.0
34	6275.0
35	6998.0
36	7505.5
37	8013.0
38	8647.0
39	9281.0
40	10778.0
41	12275.0
42	13820.0
43	15365.0
44	17922.5
45	20480.0
46	53262.5
47	86045.0
48	62084.5
49	38124.0
50	37883.0
51	37642.0
52	32710.5
53	27779.0
54	26523.0
55	25267.0
56	23650.0
57	22033.0
58	20753.5
59	19474.0
60	18542.0
61	17610.0
62	16472.5
63	15335.0
64	13650.5
65	11966.0
66	10501.5
67	9037.0
68	8119.5
69	7202.0
70	5925.0
71	4648.0
72	3859.0
73	3070.0
74	2579.5
75	1655.0
76	1221.0
77	969.0
78	717.0
79	520.5
80	324.0
81	266.0
82	208.0
83	153.5
84	99.0
85	74.0
86	49.0
87	28.5
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	439251.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.18509006756632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54990375585386	24.45939904219566
2	8.430883530699106	5.76421025692221
3	3.792612413232736	3.8895239081319346
4	2.6889045990514537	3.67681783606669
5	2.3065753521524215	3.9425243080479517
6	1.9529771511597787	4.005761988737768
7	1.7308822120158298	4.14192550198767
8	1.5143754893204706	4.141524999882808
9	1.2817162938293307	3.9434028251060673
>10	4.664876247520441	22.016759235343546
>50	0.04481882167460503	1.1042809127497775
>100	0.02876431838817935	2.0077834777268677
>500	0.0060204387324096316	1.4001888649341245
>1k	0.004682563458540824	4.939421767528514
>5k	0.0	0.0
>10k+	0.0020068129108032104	10.566475074638428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	16702	3.802381781714783	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	15173	3.454289233262986	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	14332	3.262826948601141	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	4245	0.9664178339946865	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3333	0.758791670366146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	3201	0.7287405151041204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	3055	0.695502116102183	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	2749	0.6258380743583964	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	2510	0.5714272705127592	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	2507	0.5707442897113496	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	994	0.22629430553373814	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	878	0.19988571454589743	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	873	0.19874741321021464	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	663	0.1509387571115376	Illumina Paired End PCR Primer 2 (95% over 21bp)
TAGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	587	0.13363657680915922	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	574	0.13067699333638397	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	530	0.12065994158237546	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	518	0.11792801837673676	No Hit
TAGTGAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	506	0.11519609517109806	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	466	0.10608968448563579	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15663026378995154	0.0
2	0.0	0.0	0.0	1.5143960969923802	0.0
3	0.0	0.0	0.0	1.8001097322487598	0.0
4	0.0	0.0	0.0	2.8084170553965726	0.0
5	0.0	0.0	0.0	7.1335068104568915	0.0
6	0.0	0.0	0.0	8.09514377884171	0.0
7	0.0	0.0	0.0	9.471577753949337	0.0
8	0.0	0.0	0.0	10.954329073809735	0.0
9	0.0	0.0	0.0	11.392119767513336	0.0
10	0.0	0.0	0.0	15.403948994993751	0.0
11	0.0	0.0	0.0	16.47918843667971	0.0
12	0.0	0.0	0.0	21.360224564087503	0.0
13	0.0	0.0	0.0	21.89818577533119	0.0
14	0.0	0.0	0.0	22.26790604916096	0.0
15	0.0	0.0	0.0	23.104557530887806	0.0
16	0.0	0.0	0.0	23.873593913275098	0.0
17	0.0	0.0	0.0	24.521970354080015	0.0
18	0.0	0.0	0.0	25.093852945127047	0.0
19	0.0	0.0	0.0	26.322535406863047	0.0
20	0.0	0.0	0.0	26.822022032960653	0.0
21	0.0	0.0	0.0	27.30762138276293	0.0
22	0.0	0.0	0.0	27.88769974342688	0.0
23	0.0	0.0	0.0	28.43249076268466	0.0
24	0.0	0.0	0.0	28.880526168409407	0.0
25	0.0	0.0	0.0	29.24933580117063	0.0
26	0.0	0.0	0.0	29.62679652408304	0.0
27	0.0	0.0	0.0	30.056619108436863	0.0
28	0.0	0.0	0.0	30.392190342196148	0.0
29	0.0	0.0	0.0	30.737550967442306	0.0
30	0.0	0.0	0.0	31.148250089356655	0.0
31	0.0	0.0	0.0	31.521157606926337	0.0
32	0.0	0.0	0.0	31.909318362394167	0.0
33	0.0	0.0	0.0	32.28154289916244	0.0
34	0.0	0.0	0.0	32.653995096197846	0.0
35	0.0	0.0	0.0	33.035553703918715	0.0
36	0.0	0.0	0.0	33.41483570896822	0.0
37	0.0	0.0	0.0	33.79001982920927	0.0
38	0.0	0.0	0.0	34.16861885345736	0.0
39	0.0	0.0	0.0	34.519215664847664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACC	20	7.028521E-4	45.000004	45
TAAAGCG	40	6.7993824E-9	45.000004	1
GGGTCGA	20	7.028521E-4	45.000004	8
GCGTTAG	20	7.028521E-4	45.000004	1
GTTGCGC	20	7.028521E-4	45.000004	32
AATGCGG	25	3.8868573E-5	45.0	2
CGATCGA	85	0.0	45.0	41
ACGGGTA	60	0.0	44.999996	5
TGTTGCG	30	2.1624273E-6	44.999996	1
TACGAAT	30	2.1624273E-6	44.999996	12
GGTATTC	30	2.1624273E-6	44.999996	8
CGTTTTT	1840	0.0	43.28804	1
GATGAAT	1515	0.0	42.92079	20
TACGGCT	1830	0.0	42.663937	7
CATCGAA	95	0.0	42.63158	42
ATGAATG	1515	0.0	42.62376	21
CCGATGA	1425	0.0	42.473686	18
CCGATCG	85	0.0	42.35294	40
ACGGCTG	1850	0.0	42.324326	8
CGATGAA	1465	0.0	41.928326	19
>>END_MODULE