FastQCFastQC Report
Sat 18 Jun 2016
SRR3551926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273995
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC68792.5106297560174453No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC58482.1343455172539647No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG56162.0496724392780887No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC27290.9960035767076042No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.7350499096698845No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT15130.5521998576616362No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT12830.4682567200131389No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10810.39453274694793694No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC9660.35256117812368837No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA9260.33796237157612363No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC9200.33577255059398897No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT5630.20547820215697365No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG3430.1251847661453676No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC3360.12262997499954378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA302.1594296E-645.00000424
CGATGTT302.1594296E-645.00000410
ATTTAGG253.8830032E-545.02
CGTATTC207.023872E-445.028
CGTATGG253.8830032E-545.02
CCGCTTA253.8830032E-545.041
AGACGCA207.023872E-445.013
GCGATCC207.023872E-445.033
ATTGGCG253.8830032E-545.012
ATTGGAT207.023872E-445.022
CGTAAGC406.7830115E-945.043
ATTGCGG253.8830032E-545.02
TCGGTTG207.023872E-445.01
GCGTAAG207.023872E-445.01
CCCGCTT253.8830032E-545.040
ATCTGCG207.023872E-445.044
CGCTTAT207.023872E-445.035
CGTTTTT13050.044.1379281
TACGGCT6850.043.686137
ACGGCTG7200.041.5625048