##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551926_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	273995
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01653679811675	33.0	31.0	34.0	30.0	34.0
2	32.117899961678134	33.0	31.0	34.0	30.0	34.0
3	32.1137320024088	33.0	31.0	34.0	30.0	34.0
4	35.74467052318473	37.0	35.0	37.0	35.0	37.0
5	35.78185003375974	37.0	35.0	37.0	35.0	37.0
6	35.68284822715743	37.0	35.0	37.0	35.0	37.0
7	35.79612766656326	37.0	35.0	37.0	35.0	37.0
8	35.133378346320185	37.0	35.0	37.0	32.0	37.0
9	36.86827131881969	39.0	37.0	39.0	32.0	39.0
10	36.69001624117229	38.0	35.0	39.0	32.0	39.0
11	37.007193561926314	39.0	37.0	39.0	34.0	39.0
12	36.99170057847771	39.0	37.0	39.0	33.0	39.0
13	37.06803773791493	39.0	37.0	39.0	33.0	39.0
14	38.11074654646983	40.0	37.0	41.0	33.0	41.0
15	38.09064398985383	40.0	37.0	41.0	33.0	41.0
16	38.307921677402874	40.0	37.0	41.0	34.0	41.0
17	38.18664209200898	40.0	37.0	41.0	34.0	41.0
18	38.049146882242376	40.0	37.0	41.0	34.0	41.0
19	37.952542929615504	40.0	37.0	41.0	34.0	41.0
20	37.89849814777642	40.0	36.0	41.0	34.0	41.0
21	37.911699118597056	40.0	36.0	41.0	34.0	41.0
22	37.99775543349331	40.0	36.0	41.0	34.0	41.0
23	38.017533166663625	40.0	36.0	41.0	34.0	41.0
24	37.85037683169401	40.0	35.0	41.0	33.0	41.0
25	37.6454387853793	39.0	35.0	41.0	33.0	41.0
26	37.745834778006895	40.0	35.0	41.0	33.0	41.0
27	37.7428274238581	40.0	35.0	41.0	33.0	41.0
28	37.56838263471961	40.0	35.0	41.0	33.0	41.0
29	37.67066552309349	40.0	36.0	41.0	33.0	41.0
30	37.31608241026296	40.0	35.0	41.0	33.0	41.0
31	37.221281410244714	39.0	35.0	41.0	32.0	41.0
32	37.148812934542605	40.0	35.0	41.0	32.0	41.0
33	36.98678078067118	40.0	35.0	41.0	31.0	41.0
34	36.82539462398949	40.0	35.0	41.0	31.0	41.0
35	36.73904268326064	40.0	35.0	41.0	31.0	41.0
36	36.62836913082356	40.0	35.0	41.0	31.0	41.0
37	36.65362871585248	40.0	35.0	41.0	31.0	41.0
38	36.403821237613826	39.0	35.0	41.0	30.0	41.0
39	36.35523275972189	39.0	35.0	41.0	30.0	41.0
40	36.20193069216592	39.0	35.0	41.0	30.0	41.0
41	36.012584171244	39.0	35.0	41.0	29.0	41.0
42	36.25509589591051	39.0	35.0	41.0	30.0	41.0
43	36.18835380207668	39.0	35.0	41.0	30.0	41.0
44	36.27269110750196	39.0	35.0	41.0	30.0	41.0
45	36.22712093286374	39.0	35.0	41.0	30.0	41.0
46	36.1442325589883	39.0	35.0	41.0	30.0	41.0
47	35.993240752568475	39.0	35.0	41.0	29.0	41.0
48	36.00573003156992	39.0	35.0	41.0	30.0	41.0
49	36.01297103961751	39.0	35.0	41.0	30.0	41.0
50	35.89984853738207	39.0	35.0	41.0	29.0	41.0
51	35.04676362707349	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	2.0
10	3.0
11	5.0
12	7.0
13	5.0
14	16.0
15	18.0
16	20.0
17	34.0
18	74.0
19	169.0
20	300.0
21	631.0
22	946.0
23	1413.0
24	1806.0
25	2402.0
26	3211.0
27	3597.0
28	3664.0
29	3543.0
30	3885.0
31	4663.0
32	6064.0
33	8674.0
34	16097.0
35	21449.0
36	19518.0
37	30485.0
38	56440.0
39	84829.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.73590758955456	15.539334659391596	17.489005273818865	19.235752477234985
2	30.36989726089892	31.005675286045363	20.441613898063835	18.18281355499188
3	27.60159856931696	31.014799540137595	25.797916020365335	15.585685870180113
4	26.32931257869669	20.686508877899232	34.638588295406855	18.345590247997226
5	30.07244657749229	23.732184893884924	25.424186572747676	20.771181955875107
6	23.275607219109837	35.95649555648826	25.870545082939472	14.897352141462436
7	65.01578495957956	6.2596032774320705	21.885435865617985	6.8391758973703904
8	62.463548604901554	13.56192631252395	15.336776218544134	8.637748864030366
9	58.05288417671856	7.76656508330444	16.2871585247906	17.893392215186406
10	40.69599810215515	18.428803445318344	24.0081023376339	16.867096114892608
11	31.532327232248765	21.61900764612493	27.181882881074472	19.666782240551836
12	26.35011587802697	18.664574171061517	30.70019525903757	24.28511469187394
13	24.893519954743702	22.59639774448439	34.88530812606069	17.624774174711217
14	20.46752677968576	27.663643497144108	29.087027135531667	22.78180258763846
15	16.740086497928793	22.799321155495537	39.0908593222504	21.36973302432526
16	19.518969324257743	23.471961167174584	29.989963320498546	27.019106188069124
17	19.954743699702547	24.72855344075622	31.545831128305263	23.77087173123597
18	20.33504261026661	23.162466468366212	31.853865946458875	24.6486249749083
19	20.966805963612476	25.17673680176646	28.21584335480574	25.640613879815326
20	22.607346849395064	24.51285607401595	34.10317706527491	18.776620011314073
21	22.50114053176153	28.508549426084418	29.268052336721475	19.72225770543258
22	20.200368619865326	22.344203361375207	32.026496833883826	25.428931184875637
23	21.230679391959708	26.369094326538807	30.479388310005657	21.92083797149583
24	22.671581598204344	22.3511377944853	29.787404879651085	25.18987572765926
25	18.859468238471504	30.62647128597237	27.810361502947135	22.70369897260899
26	19.977736820014965	23.84970528659282	31.39765324184748	24.774904651544734
27	22.949688862935457	25.684775269621706	27.96875855398821	23.396777313454624
28	17.591926859979196	24.58293034544426	34.32215916348839	23.50298363108816
29	23.812843300060223	21.2576871840727	30.58668953813026	24.34277997773682
30	19.8087556342269	26.824942060986512	30.06076753225424	23.305534772532347
31	22.644938776255042	23.704447161444552	28.704173433821783	24.946440628478623
32	23.96649573897334	25.868355261957337	28.757459077720398	21.40768992134893
33	20.477745944269056	26.38040840161317	26.040986149382288	27.100859504735485
34	23.459187211445464	25.60557674410117	27.789923173780544	23.14531287067282
35	19.757659811310425	23.937298125878208	26.13916312341466	30.165878939396706
36	23.367579700359496	27.987737002500047	26.93479808025694	21.70988521688352
37	18.686107410719174	23.654081278855454	32.796218909104184	24.863592401321192
38	18.73793317396303	27.335535319987592	28.289932298034635	25.636599208014744
39	21.37046296465264	27.689191408602348	29.900545630394713	21.039799996350297
40	20.797459807660722	26.227485903027425	27.86985163962846	25.105202649683388
41	19.25436595558313	30.232303509188124	24.857752878702165	25.655577656526578
42	19.99927005967262	23.103706272012264	30.62428146499024	26.272742203324878
43	23.09202722677421	26.44683297140459	26.513622511359696	23.947517290461505
44	21.106954506469098	23.298235369258563	30.23777806164346	25.357032062628882
45	20.32263362470118	21.66061424478549	28.80490519899998	29.211846931513346
46	25.964342415007575	25.072355334951368	27.609627912918118	21.35367433712294
47	18.37807259256556	23.72160075913794	35.113414478366394	22.78691216993011
48	21.039799996350297	25.184766145367615	27.851968101607692	25.92346575667439
49	20.021898209821348	20.647092100220807	34.81085421266811	24.52015547728973
50	21.497472581616453	21.95441522655523	30.677567108888848	25.870545082939472
51	20.264238398510923	21.47703425244986	27.715104290224275	30.54362305881494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	178.0
2	299.0
3	2089.5
4	3880.0
5	2689.5
6	1499.0
7	1448.0
8	1397.0
9	1403.0
10	1409.0
11	1369.0
12	1329.0
13	1250.5
14	1172.0
15	1124.5
16	1077.0
17	1034.5
18	992.0
19	901.0
20	810.0
21	870.5
22	931.0
23	926.5
24	922.0
25	1006.5
26	1275.5
27	1460.0
28	1851.0
29	2242.0
30	2558.0
31	2874.0
32	3376.5
33	3879.0
34	4411.5
35	4944.0
36	5145.0
37	5346.0
38	6425.5
39	7505.0
40	8604.0
41	9703.0
42	12146.0
43	14589.0
44	28416.0
45	42243.0
46	34401.0
47	26559.0
48	27054.0
49	27549.0
50	23580.0
51	19611.0
52	18061.0
53	16511.0
54	14680.0
55	12849.0
56	11918.5
57	10988.0
58	10358.5
59	9729.0
60	9086.5
61	8444.0
62	7412.0
63	6380.0
64	5859.5
65	5339.0
66	5108.0
67	4877.0
68	4406.0
69	3935.0
70	3345.0
71	2755.0
72	2620.0
73	2485.0
74	2071.0
75	1405.5
76	1154.0
77	925.0
78	696.0
79	552.0
80	408.0
81	302.5
82	197.0
83	157.5
84	118.0
85	88.5
86	59.0
87	50.5
88	42.0
89	22.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	273995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.16449205277468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19372068924997	25.34790780853665
2	10.06099840827271	6.874578003248234
3	3.6182418357209243	3.7084618332451322
4	1.7669241205439647	2.4146426029672075
5	1.134506297471397	1.9379915691892187
6	0.8663696866754266	1.7759448165112501
7	0.7584740783471675	1.8139017135349182
8	0.5811407022829002	1.5883501523750434
9	0.532000128192802	1.6357962736546285
>10	6.394684271811471	38.784284384751544
>50	0.05127712078966766	1.1784886585521634
>100	0.028843380444188056	1.8671873574335296
>500	0.004273093399138971	1.2317743024507748
>1k	0.005341366748923715	3.146042811000201
>5k	0.0032048200493542288	6.694647712549499
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	6879	2.5106297560174453	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	5848	2.1343455172539647	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	5616	2.0496724392780887	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2729	0.9960035767076042	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.7350499096698845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	1513	0.5521998576616362	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	1283	0.4682567200131389	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1081	0.39453274694793694	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	966	0.35256117812368837	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	926	0.33796237157612363	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	920	0.33577255059398897	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	563	0.20547820215697365	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG	343	0.1251847661453676	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	336	0.12262997499954378	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21460245624920163	0.0
2	0.0	0.0	0.0	1.8044124892790014	0.0
3	0.0	0.0	0.0	2.196755415244804	0.0
4	0.0	0.0	0.0	3.06757422580704	0.0
5	0.0	0.0	0.0	6.531871019544152	0.0
6	0.0	0.0	0.0	7.428967681892005	0.0
7	0.0	0.0	0.0	8.776072556068542	0.0
8	0.0	0.0	0.0	10.223179255095896	0.0
9	0.0	0.0	0.0	10.643259913502071	0.0
10	0.0	0.0	0.0	13.635650285589152	0.0
11	0.0	0.0	0.0	15.134217777696673	0.0
12	0.0	0.0	0.0	18.945966167265826	0.0
13	0.0	0.0	0.0	19.577364550448	0.0
14	0.0	0.0	0.0	19.874085293527255	0.0
15	0.0	0.0	0.0	20.65767623496779	0.0
16	0.0	0.0	0.0	21.54382379240497	0.0
17	0.0	0.0	0.0	22.500045621270463	0.0
18	0.0	0.0	0.0	23.372324312487454	0.0
19	3.649701636891184E-4	0.0	0.0	24.546798299239036	0.0
20	3.649701636891184E-4	0.0	0.0	25.223088012554975	0.0
21	3.649701636891184E-4	0.0	0.0	25.93587474223982	0.0
22	3.649701636891184E-4	0.0	0.0	26.67311447289184	0.0
23	3.649701636891184E-4	0.0	0.0	27.353418858008357	0.0
24	3.649701636891184E-4	0.0	0.0	27.91510793992591	0.0
25	3.649701636891184E-4	0.0	0.0	28.442124856293	0.0
26	3.649701636891184E-4	0.0	0.0	28.909651635978758	0.0
27	3.649701636891184E-4	0.0	0.0	29.435573641854777	0.0
28	3.649701636891184E-4	0.0	0.0	29.89799083924889	0.0
29	3.649701636891184E-4	0.0	0.0	30.39982481432143	0.0
30	3.649701636891184E-4	0.0	0.0	30.90968813299513	0.0
31	3.649701636891184E-4	0.0	0.0	31.404952645121263	0.0
32	3.649701636891184E-4	0.0	0.0	31.894742604792057	0.0
33	3.649701636891184E-4	0.0	0.0	32.36993375791529	0.0
34	3.649701636891184E-4	0.0	0.0	32.828336283508825	0.0
35	3.649701636891184E-4	0.0	0.0	33.303162466468365	0.0
36	3.649701636891184E-4	0.0	0.0	33.75901020091607	0.0
37	3.649701636891184E-4	0.0	0.0	34.197704337670395	0.0
38	3.649701636891184E-4	0.0	0.0	34.6334787131152	0.0
39	3.649701636891184E-4	0.0	0.0	35.04005547546488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	30	2.1594296E-6	45.000004	24
CGATGTT	30	2.1594296E-6	45.000004	10
ATTTAGG	25	3.8830032E-5	45.0	2
CGTATTC	20	7.023872E-4	45.0	28
CGTATGG	25	3.8830032E-5	45.0	2
CCGCTTA	25	3.8830032E-5	45.0	41
AGACGCA	20	7.023872E-4	45.0	13
GCGATCC	20	7.023872E-4	45.0	33
ATTGGCG	25	3.8830032E-5	45.0	12
ATTGGAT	20	7.023872E-4	45.0	22
CGTAAGC	40	6.7830115E-9	45.0	43
ATTGCGG	25	3.8830032E-5	45.0	2
TCGGTTG	20	7.023872E-4	45.0	1
GCGTAAG	20	7.023872E-4	45.0	1
CCCGCTT	25	3.8830032E-5	45.0	40
ATCTGCG	20	7.023872E-4	45.0	44
CGCTTAT	20	7.023872E-4	45.0	35
CGTTTTT	1305	0.0	44.137928	1
TACGGCT	685	0.0	43.68613	7
ACGGCTG	720	0.0	41.562504	8
>>END_MODULE