FastQCFastQC Report
Sat 18 Jun 2016
SRR3551925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307951
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC64972.109751226656189No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC56831.8454234602258153No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG51571.674617065702011No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36151.17388805361892No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC23040.7481709752525564No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATGCTCAT15840.5143675454861325No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT12090.39259492581612015No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC10750.34908150972070234No Hit
GAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT10150.3295978905735003No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTA9680.31433572224152545No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC8880.2883575633785895No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT6250.20295436611668738No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC3380.10975772119590453No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3230.10488681640910405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC551.8189894E-1245.00000434
GCATAAG207.025236E-445.01
TCCGATG207.025236E-445.027
TAGCGAG207.025236E-445.01
AATATCG207.025236E-445.038
ATCCGAA207.025236E-445.032
ATATCGG207.025236E-445.039
GACGGGT406.7866495E-945.04
CCAGTCG207.025236E-445.010
CAATACG207.025236E-445.01
GCCAATC207.025236E-445.015
AGTCGAG207.025236E-445.012
TATTGCG302.1603082E-644.9999961
CGTTTTT15700.043.566881
GAATGAC1350.043.3333321
TACGGCT6600.043.2954527
TTACGGG750.042.03
ACGGCTG6850.041.715338
GATGAAT3350.041.64179220
TGAATGA3300.041.59090822