##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307951
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11965215245282	33.0	31.0	34.0	30.0	34.0
2	32.212498092228955	33.0	31.0	34.0	30.0	34.0
3	32.20732194407552	33.0	31.0	34.0	30.0	34.0
4	35.80314075940653	37.0	35.0	37.0	35.0	37.0
5	35.841568950904524	37.0	35.0	37.0	35.0	37.0
6	35.74571928650987	37.0	35.0	37.0	35.0	37.0
7	35.88896610174995	37.0	35.0	37.0	35.0	37.0
8	35.24510068160194	37.0	35.0	37.0	32.0	37.0
9	37.04478959314956	39.0	37.0	39.0	33.0	39.0
10	36.84456293371348	39.0	35.0	39.0	33.0	39.0
11	37.145016577312624	39.0	37.0	39.0	34.0	39.0
12	37.11023182259515	39.0	37.0	39.0	34.0	39.0
13	37.20700371162945	39.0	37.0	39.0	34.0	39.0
14	38.297774645966406	40.0	38.0	41.0	34.0	41.0
15	38.26323018921841	40.0	38.0	41.0	34.0	41.0
16	38.434877626635405	40.0	38.0	41.0	34.0	41.0
17	38.30741579017441	40.0	37.0	41.0	34.0	41.0
18	38.178765452945434	40.0	37.0	41.0	34.0	41.0
19	38.095063825089056	40.0	37.0	41.0	34.0	41.0
20	38.041996941071794	40.0	36.0	41.0	34.0	41.0
21	38.03662270945702	40.0	36.0	41.0	34.0	41.0
22	38.10639354962316	40.0	36.0	41.0	34.0	41.0
23	38.13648924666587	40.0	36.0	41.0	34.0	41.0
24	37.9801851593273	40.0	36.0	41.0	34.0	41.0
25	37.80018899110573	40.0	35.0	41.0	34.0	41.0
26	37.88105899964605	40.0	36.0	41.0	34.0	41.0
27	37.913729781686044	40.0	36.0	41.0	34.0	41.0
28	37.76821961935503	40.0	35.0	41.0	34.0	41.0
29	37.83330789638612	40.0	36.0	41.0	34.0	41.0
30	37.50207338180425	40.0	35.0	41.0	33.0	41.0
31	37.39497842189179	40.0	35.0	41.0	33.0	41.0
32	37.33747901451854	40.0	35.0	41.0	33.0	41.0
33	37.18565940685369	40.0	35.0	41.0	33.0	41.0
34	37.03437884598524	40.0	35.0	41.0	32.0	41.0
35	36.94207844754523	40.0	35.0	41.0	32.0	41.0
36	36.8017866478758	40.0	35.0	41.0	31.0	41.0
37	36.75083048926615	40.0	35.0	41.0	31.0	41.0
38	36.5787251867992	40.0	35.0	41.0	31.0	41.0
39	36.51545538088852	40.0	35.0	41.0	31.0	41.0
40	36.43631291991258	39.0	35.0	41.0	30.0	41.0
41	36.22566577150261	39.0	35.0	41.0	30.0	41.0
42	36.422333423174464	39.0	35.0	41.0	31.0	41.0
43	36.345759552656105	39.0	35.0	41.0	30.0	41.0
44	36.434452234284024	39.0	35.0	41.0	31.0	41.0
45	36.42719133888183	39.0	35.0	41.0	31.0	41.0
46	36.312783527249465	39.0	35.0	41.0	30.0	41.0
47	36.21054323577452	39.0	35.0	41.0	30.0	41.0
48	36.245162379729244	39.0	35.0	41.0	30.0	41.0
49	36.24082077992927	39.0	35.0	41.0	31.0	41.0
50	36.147848196628686	39.0	35.0	41.0	30.0	41.0
51	35.31002659514014	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	5.0
12	8.0
13	7.0
14	10.0
15	14.0
16	19.0
17	40.0
18	76.0
19	106.0
20	265.0
21	588.0
22	940.0
23	1321.0
24	1756.0
25	2466.0
26	3436.0
27	4201.0
28	4051.0
29	3948.0
30	3912.0
31	4801.0
32	6356.0
33	9021.0
34	18127.0
35	22948.0
36	21468.0
37	32564.0
38	60886.0
39	104568.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22375312955633	16.555880643349106	18.51820581845813	19.702160408636438
2	31.257245470870366	30.717224493507082	20.5600891050849	17.46544093053765
3	28.08044136891908	29.223155631902475	27.0520310049326	15.644371994245837
4	27.05820081766255	20.73122022659449	34.55387383057694	17.656705125166017
5	29.841111085854564	24.652623306954677	25.29038710703976	20.215878500151
6	23.081269422732838	35.153319846339194	26.599036859760155	15.166373871167815
7	67.41234806836152	6.011670687869175	20.6113959688392	5.964585274930102
8	65.60751548135904	12.449058454104712	14.730914983227853	7.2125110813083895
9	60.73920851044484	7.589194384820962	15.927858652837626	15.743738451896569
10	40.99710668255664	20.08955970267997	23.172517705738898	15.740815909024489
11	31.543654672334238	22.12170117973314	26.67437352046267	19.660270627469956
12	26.932531474163095	19.00756938603869	30.552587911713225	23.507311228084987
13	25.07834038532104	21.372231296537436	34.920490597530126	18.628937720611397
14	20.297709700569246	26.988709242704196	29.394611480397852	23.3189695763287
15	18.39351065591604	22.856883075554226	37.08122396095483	21.66838230757491
16	19.99506414981604	23.918740319076736	30.07004361083419	26.01615192027303
17	20.196719607989582	25.964845056518733	31.41701114787742	22.421424187614264
18	19.728138567499375	23.941795935067592	31.87747401372296	24.45259148371007
19	21.521605709999317	24.52468087455472	29.618348373604892	24.335365041841072
20	23.12705592772876	25.142961055492595	33.46701260914886	18.262970407629787
21	22.957548441148106	28.282421554078407	30.043091271013893	18.71693873375959
22	20.362655097726588	22.31913518709145	32.67337985588617	24.644829859295797
23	21.63168815818101	25.71318164253404	31.275105455088635	21.380024744196316
24	22.946182996645568	22.76920678939182	29.70310211689522	24.58150809706739
25	19.64306009722326	29.660887608742946	28.15935002646525	22.536702267568543
26	18.97217414458794	23.890489071313294	32.64512860812272	24.49220817597605
27	23.039379641566356	26.35776470932064	28.696448460956454	21.906407188156557
28	17.978834295066424	25.29363437689762	34.07295316462684	22.654578163409113
29	21.70345282203987	21.99895437910577	32.48276511522937	23.81482768362499
30	19.417374842101502	28.464268666118958	30.386652421976223	21.73170406980331
31	22.1275462654773	23.173816613682046	30.46588580650818	24.232751314332475
32	25.276423846650932	26.213910654617127	26.85492172456008	21.654743774171862
33	20.54417748278135	24.532799049199387	26.604557218518533	28.318466249500734
34	21.77911420972817	22.27919376783969	32.61817626830242	23.323515754129716
35	20.75070384574169	24.67990037376076	27.144253468896025	27.42514231160152
36	19.868420625359228	27.73623076398518	30.335670285207712	22.05967832544788
37	21.454062496955682	26.884471880266663	30.752295007972048	20.909170614805603
38	20.505534971472734	25.04197096291293	27.993739263714033	26.4587548019003
39	24.922471432143425	23.581348980844353	29.283554851258803	22.212624735753415
40	20.59970579735088	22.125922630548366	30.242148913301143	27.032222658799615
41	18.682192946280416	26.533766735617032	29.552753522475978	25.231286795626577
42	18.959834519128044	23.237787829882027	31.008504599757753	26.793873051232175
43	21.796649466960652	25.048790229614454	28.48115446937987	24.673405834045028
44	21.416394166604427	23.007881123945044	30.32592847563411	25.24979623381642
45	20.425976859954993	21.19590454325526	29.371231137421212	29.006887459368535
46	24.777967923468346	24.3347155878695	27.96548801595059	22.921828472711567
47	18.249331874226744	22.77602605609334	35.68424846810044	23.290393601579474
48	20.21880104302308	23.58005007290121	30.252215449860532	25.94893343421518
49	20.33570275790629	21.214738708430886	34.17751525405016	24.272043279612664
50	21.616750716834822	21.47516975103182	31.090011073190215	25.818068458943145
51	20.085338251864744	20.66822319135187	29.084821935957343	30.16161662082604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	204.5
2	329.0
3	3226.0
4	6123.0
5	4030.0
6	1937.0
7	1842.5
8	1748.0
9	1704.5
10	1661.0
11	1587.5
12	1514.0
13	1466.5
14	1419.0
15	1309.5
16	1200.0
17	1151.0
18	1102.0
19	1037.0
20	972.0
21	1010.0
22	1048.0
23	1076.0
24	1104.0
25	1190.5
26	1710.5
27	2144.0
28	2391.5
29	2639.0
30	3079.5
31	3520.0
32	4128.5
33	4737.0
34	5276.0
35	5815.0
36	6209.5
37	6604.0
38	7169.5
39	7735.0
40	9416.0
41	11097.0
42	12978.0
43	14859.0
44	28893.5
45	42928.0
46	35840.0
47	28752.0
48	29069.0
49	29386.0
50	25453.5
51	21521.0
52	19129.0
53	16737.0
54	15702.0
55	14667.0
56	13748.0
57	12829.0
58	12652.5
59	12476.0
60	11494.5
61	10513.0
62	10155.5
63	9798.0
64	8643.0
65	7488.0
66	6569.0
67	5650.0
68	4935.5
69	4221.0
70	3963.5
71	3706.0
72	3042.0
73	2378.0
74	2078.5
75	1438.0
76	1097.0
77	847.0
78	597.0
79	508.0
80	419.0
81	303.5
82	188.0
83	140.5
84	93.0
85	61.5
86	30.0
87	22.0
88	14.0
89	11.5
90	9.0
91	6.5
92	4.0
93	5.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	307951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.94082502456392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.07376000689138	23.783612272071853
2	10.647225970097363	7.227512672961348
3	3.865685288596982	3.9361364394090304
4	2.212710380159868	3.0040486337216956
5	1.6581506162606081	2.8139499965437063
6	1.4036632707550907	2.858489367966337
7	1.2829398662446116	3.0480826262072056
8	1.0953859405741013	2.9742642034714355
9	1.0604690857162957	3.239387611403046
>10	6.58528338343659	32.983716629677886
>50	0.06783809570576464	1.5345358240490543
>100	0.03491666690737886	2.2283275061930334
>500	0.002992857163489616	0.8400669416296789
>1k	0.005985714326979232	3.657558687417892
>5k	0.002992857163489616	5.8703105872768
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	6497	2.109751226656189	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	5683	1.8454234602258153	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	5157	1.674617065702011	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3615	1.17388805361892	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2304	0.7481709752525564	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATGCTCAT	1584	0.5143675454861325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	1209	0.39259492581612015	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	1075	0.34908150972070234	No Hit
GAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1015	0.3295978905735003	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATGCTCATCGTA	968	0.31433572224152545	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTC	888	0.2883575633785895	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	625	0.20295436611668738	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	338	0.10975772119590453	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	323	0.10488681640910405	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2065263629603411	0.0
2	0.0	0.0	0.0	1.4976408584482597	0.0
3	0.0	0.0	0.0	1.8645823523872305	0.0
4	0.0	0.0	0.0	2.5883988037057843	0.0
5	0.0	0.0	0.0	5.623946666839855	0.0
6	0.0	0.0	0.0	6.506879341193891	0.0
7	0.0	0.0	0.0	7.692132839315345	0.0
8	0.0	0.0	0.0	9.064104354264153	0.0
9	0.0	0.0	0.0	9.45897236898078	0.0
10	0.0	0.0	0.0	12.03600572818403	0.0
11	0.0	0.0	0.0	13.458959379901348	0.0
12	0.0	0.0	0.0	16.81014187322009	0.0
13	0.0	0.0	0.0	17.40666534611026	0.0
14	0.0	0.0	0.0	17.693074547574128	0.0
15	0.0	0.0	0.0	18.410721186162732	0.0
16	0.0	0.0	0.0	19.2514393523645	0.0
17	0.0	0.0	0.0	20.11521313455712	0.0
18	0.0	0.0	0.0	20.91209315767768	0.0
19	0.0	0.0	0.0	22.028504534812356	3.2472698578669984E-4
20	0.0	0.0	0.0	22.670165058726877	3.2472698578669984E-4
21	0.0	0.0	0.0	23.355014271751024	3.2472698578669984E-4
22	0.0	0.0	0.0	24.099937977145714	3.2472698578669984E-4
23	0.0	0.0	0.0	24.752314491591196	3.2472698578669984E-4
24	0.0	0.0	0.0	25.31149436111589	3.2472698578669984E-4
25	0.0	0.0	0.0	25.84891752259288	3.2472698578669984E-4
26	0.0	0.0	0.0	26.315874928154155	3.2472698578669984E-4
27	0.0	0.0	0.0	26.829593019668714	3.2472698578669984E-4
28	0.0	0.0	0.0	27.29817406015892	3.2472698578669984E-4
29	0.0	0.0	0.0	27.767079827634916	3.2472698578669984E-4
30	0.0	0.0	0.0	28.310023347870278	3.2472698578669984E-4
31	0.0	0.0	0.0	28.829586525129	3.2472698578669984E-4
32	0.0	0.0	0.0	29.3202490006527	3.2472698578669984E-4
33	0.0	0.0	0.0	29.810911476176404	3.2472698578669984E-4
34	0.0	0.0	0.0	30.290208507197573	3.2472698578669984E-4
35	0.0	0.0	0.0	30.804576052683707	3.2472698578669984E-4
36	0.0	0.0	0.0	31.260168013742447	3.2472698578669984E-4
37	0.0	0.0	0.0	31.762845387740256	3.2472698578669984E-4
38	0.0	0.0	0.0	32.24993586642031	3.2472698578669984E-4
39	0.0	0.0	0.0	32.73670161811457	3.2472698578669984E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	55	1.8189894E-12	45.000004	34
GCATAAG	20	7.025236E-4	45.0	1
TCCGATG	20	7.025236E-4	45.0	27
TAGCGAG	20	7.025236E-4	45.0	1
AATATCG	20	7.025236E-4	45.0	38
ATCCGAA	20	7.025236E-4	45.0	32
ATATCGG	20	7.025236E-4	45.0	39
GACGGGT	40	6.7866495E-9	45.0	4
CCAGTCG	20	7.025236E-4	45.0	10
CAATACG	20	7.025236E-4	45.0	1
GCCAATC	20	7.025236E-4	45.0	15
AGTCGAG	20	7.025236E-4	45.0	12
TATTGCG	30	2.1603082E-6	44.999996	1
CGTTTTT	1570	0.0	43.56688	1
GAATGAC	135	0.0	43.333332	1
TACGGCT	660	0.0	43.295452	7
TTACGGG	75	0.0	42.0	3
ACGGCTG	685	0.0	41.71533	8
GATGAAT	335	0.0	41.641792	20
TGAATGA	330	0.0	41.590908	22
>>END_MODULE