FastQCFastQC Report
Sat 18 Jun 2016
SRR3551924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274161
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC73432.6783532304011146No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC61152.2304412370833195No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG57282.0892833043357735No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC24900.9082254587632813RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.9002009767983047No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT20470.7466415719230671No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT13000.47417393429408267No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT12090.44098175889349694RNA PCR Primer, Index 35 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC10910.39794135562680316RNA PCR Primer, Index 10 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA10870.3964823589058984No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTC9700.3538067048194309No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT5200.18966957371763307TruSeq Adapter, Index 10 (95% over 24bp)
CTGAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC4590.16741987372383382No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3990.14553492291026077No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATG3930.14334642782890344No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTTTGTC3660.13349819996279558No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT2800.10212977046334089No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGT2780.10140027210288846No Hit
CTGAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT2760.10067077374243602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGG453.8198777E-1045.0000042
AACGAGC302.159435E-645.00000415
GACGTCT207.0238794E-445.020
CCTCGTA207.0238794E-445.040
ACGGGTC406.7830115E-945.05
TCTAGCG253.8830112E-545.01
CGAATAT700.045.014
ATTGGGT207.0238794E-445.04
ACGTAGG502.1827873E-1145.02
CACCGCT406.7830115E-945.038
CTAGGCG207.0238794E-445.01
GATGAAT6250.043.19999720
CGATGAA6000.043.12519
ATGAATG6300.042.521
CGTTTTT14250.042.4736861
TACGGCT8350.042.305397
GACTATG750.042.024
CCGATGA6150.041.34146518
AAGGGCG1150.041.0869565
ACGGCTG8600.041.075588
Produced by FastQC (version 0.11.5)