##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551924_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	274161
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.999332509000187	33.0	31.0	34.0	30.0	34.0
2	32.10906000488764	33.0	31.0	34.0	30.0	34.0
3	32.10718519410128	33.0	31.0	34.0	30.0	34.0
4	35.74156791082612	37.0	35.0	37.0	35.0	37.0
5	35.76916483380204	37.0	35.0	37.0	35.0	37.0
6	35.653010457358995	37.0	35.0	37.0	35.0	37.0
7	35.80017216161307	37.0	35.0	37.0	35.0	37.0
8	35.02540478040276	37.0	35.0	37.0	32.0	37.0
9	36.81657857973964	39.0	37.0	39.0	32.0	39.0
10	36.71542633707931	38.0	35.0	39.0	32.0	39.0
11	37.02570022723874	39.0	37.0	39.0	34.0	39.0
12	37.01377657653715	39.0	37.0	39.0	33.0	39.0
13	37.07930741425659	39.0	37.0	39.0	33.0	39.0
14	38.16710618942884	40.0	37.0	41.0	33.0	41.0
15	38.09020976725355	40.0	37.0	41.0	33.0	41.0
16	38.31520165158429	40.0	37.0	41.0	34.0	41.0
17	38.14263151943566	40.0	37.0	41.0	33.0	41.0
18	38.04736632854418	40.0	37.0	41.0	34.0	41.0
19	37.97804209935038	40.0	37.0	41.0	34.0	41.0
20	37.84466791410886	40.0	36.0	41.0	33.0	41.0
21	37.87725095837847	40.0	36.0	41.0	33.0	41.0
22	37.94156718132776	40.0	36.0	41.0	34.0	41.0
23	37.943708259015686	40.0	36.0	41.0	34.0	41.0
24	37.80761669238149	40.0	36.0	41.0	33.0	41.0
25	37.664613128781994	39.0	35.0	41.0	33.0	41.0
26	37.72127326643833	40.0	36.0	41.0	33.0	41.0
27	37.70824077822885	40.0	36.0	41.0	33.0	41.0
28	37.48059351986606	40.0	35.0	41.0	33.0	41.0
29	37.58830760027867	40.0	35.0	41.0	33.0	41.0
30	37.23656537581932	39.0	35.0	41.0	32.0	41.0
31	37.045039228774336	39.0	35.0	41.0	31.0	41.0
32	36.9791983542517	39.0	35.0	41.0	31.0	41.0
33	36.85824023110508	40.0	35.0	41.0	31.0	41.0
34	36.676095432975515	40.0	35.0	41.0	31.0	41.0
35	36.669376753075746	40.0	35.0	41.0	31.0	41.0
36	36.34883882098475	39.0	35.0	41.0	30.0	41.0
37	36.359139337834335	39.0	35.0	41.0	30.0	41.0
38	36.0101619121611	39.0	35.0	41.0	29.0	41.0
39	36.05651059049245	39.0	35.0	41.0	30.0	41.0
40	36.02173175615788	39.0	35.0	41.0	29.0	41.0
41	35.856361772826915	39.0	35.0	41.0	27.0	41.0
42	35.96061803101097	39.0	35.0	41.0	29.0	41.0
43	35.900923180175155	39.0	35.0	41.0	29.0	41.0
44	36.044524932430214	39.0	35.0	41.0	29.0	41.0
45	35.94634174809692	39.0	35.0	41.0	28.0	41.0
46	35.87183808054392	39.0	35.0	41.0	28.0	41.0
47	35.688733992070354	39.0	35.0	41.0	28.0	41.0
48	35.6301224462998	38.0	35.0	41.0	28.0	41.0
49	35.644030332541824	38.0	35.0	40.0	28.0	41.0
50	35.5312790659503	38.0	35.0	40.0	28.0	41.0
51	34.56517155977692	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	2.0
12	7.0
13	6.0
14	7.0
15	14.0
16	24.0
17	46.0
18	96.0
19	205.0
20	499.0
21	961.0
22	1316.0
23	1588.0
24	1917.0
25	2474.0
26	3246.0
27	3642.0
28	3602.0
29	3796.0
30	4121.0
31	4990.0
32	6400.0
33	8848.0
34	16229.0
35	20279.0
36	19924.0
37	31201.0
38	57740.0
39	80954.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.844514719453166	15.498922166172433	17.819821199951853	18.836741914422547
2	29.048989462396186	33.85638365777773	20.218776558299687	16.875850321526404
3	27.291627912066268	32.1613942172665	25.2636224700085	15.283355400658738
4	26.563953297514963	20.61671791392649	36.11016884239553	16.70915994616302
5	32.0278960173037	22.904060023125098	24.495095947271857	20.572948012299342
6	22.74977111988941	37.204051633893954	25.65208034694942	14.39409689926722
7	66.01121238980015	5.5766502164786385	22.504294921597165	5.907842472124044
8	63.04871954800282	15.369071458011899	14.286131141920258	7.296077852065028
9	58.86431695244765	7.303737584849777	15.387673666203435	18.444271796499137
10	42.98277289621792	19.722352924011805	22.17966815119583	15.115206028574452
11	31.84770992227195	21.66683080379777	25.785578546912213	20.699880727018066
12	26.09926284190676	18.18347613263739	30.209986103056234	25.507274922399613
13	25.74727988298846	20.973807361367953	34.51621492480695	18.762697830836625
14	20.163699432085526	27.8179609791327	28.01127804465259	24.00706154412918
15	17.062966650982453	22.78624603791203	37.39007371580932	22.760713595296195
16	19.062521656982575	23.302730877112353	29.341153555757383	28.293593910147685
17	18.98008834225145	26.059869930442332	31.845521427190594	23.114520300115625
18	19.019116504535656	23.673680793402415	30.624705920973444	26.68249678108848
19	20.378171950058544	25.321982338844695	28.113407815115938	26.18643789598083
20	24.279529181758164	24.44184256695883	33.73236893650082	17.54625931478219
21	23.010566783751155	29.18066391645785	28.79731252804009	19.011456771750908
22	19.402832642133635	22.32447357574564	32.5356268761786	25.73706690594213
23	21.786468534911968	26.705111230262514	29.874416857248114	21.63400337757741
24	23.039017219808798	23.14588872961508	28.461378533051747	25.35371551752437
25	19.039542458628326	31.265205481450682	26.23531428613114	23.459937773789854
26	19.883936810852017	23.37823395741918	31.241861533916204	25.4959676978126
27	25.158574706103348	25.771353328883396	26.925419735119142	22.144652229894113
28	18.78713602591178	25.7812015567495	33.33989881857741	22.091763598761315
29	24.934983458624675	20.773195312243537	30.616681439008463	23.675139790123325
30	23.180539901736573	26.31045261725774	30.393090191529794	20.11591728947589
31	25.31541685360062	22.1778444052947	27.15958870882438	25.347150032280304
32	25.62326516171155	25.55177432238721	29.29701890495001	19.52794161095123
33	23.454831285266685	21.70038772837858	28.365084749472025	26.479696236882706
34	27.688110270972167	21.673761038222068	28.368002742913834	22.270125947891934
35	24.6278646488742	20.564558781154137	29.813138994970107	24.994437575001548
36	26.135737759929384	23.25020699515978	27.137703758010804	23.476351486900032
37	25.536819605997934	21.776255557865635	32.92481425147997	19.76211058465646
38	20.38400793694216	22.434627828173955	31.767100353441958	25.41426388144193
39	25.162586947085835	21.412600625180094	31.44247358304062	21.982338844693448
40	22.316084344600434	19.99555006000124	35.25191402132324	22.436451574075086
41	23.61276768030464	22.279609426577814	27.072778403930535	27.03484448918701
42	21.225849044904272	20.01779975999504	34.88570584437611	23.870645350724573
43	23.92134548677602	21.22694329244495	27.504641433318376	27.347069787460654
44	23.890706555637017	20.088561100958927	30.9121282749917	25.108604068412355
45	21.868901849643095	19.384959932302554	29.650096111408992	29.096042106645363
46	26.861223879399333	22.554995057648608	28.31949110194375	22.264289961008313
47	19.112857043853793	21.493939692370542	37.61621820754958	21.776985056226085
48	22.484963215045173	22.50465967077739	29.325469341007654	25.68490777316978
49	20.612705672944003	18.940330681606792	36.97462439953166	23.472339245917546
50	22.694329244495023	19.309456851995723	31.511411178103376	26.484802725405878
51	20.712646948325983	19.250367484799078	28.967650395205734	31.0693351716692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	218.0
2	369.0
3	2349.5
4	4330.0
5	2907.0
6	1484.0
7	1432.0
8	1380.0
9	1392.5
10	1405.0
11	1270.0
12	1135.0
13	1109.5
14	1084.0
15	1044.0
16	1004.0
17	857.0
18	710.0
19	704.0
20	698.0
21	780.5
22	863.0
23	875.0
24	887.0
25	987.5
26	1396.0
27	1704.0
28	1881.5
29	2059.0
30	2405.0
31	2751.0
32	3328.0
33	3905.0
34	4367.0
35	4829.0
36	5148.5
37	5468.0
38	6096.5
39	6725.0
40	7533.0
41	8341.0
42	9613.5
43	10886.0
44	12568.0
45	14250.0
46	28579.5
47	42909.0
48	34227.5
49	25546.0
50	25813.5
51	26081.0
52	22153.0
53	18225.0
54	16536.5
55	14848.0
56	14015.0
57	13182.0
58	12740.0
59	12298.0
60	11799.5
61	11301.0
62	9991.0
63	8681.0
64	7869.5
65	7058.0
66	6020.5
67	4983.0
68	4349.5
69	3716.0
70	3233.0
71	2750.0
72	2371.0
73	1992.0
74	1740.5
75	1130.5
76	772.0
77	665.0
78	558.0
79	366.0
80	174.0
81	153.5
82	133.0
83	78.5
84	24.0
85	20.0
86	16.0
87	9.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	274161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.53970112452172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16868508559465	26.359329007408057
2	9.028490496325793	6.41739707690007
3	3.33244386058541	3.5530217645835838
4	1.848392791165483	2.6276530943496703
5	1.356788045486268	2.4109920812952974
6	1.1730777125497762	2.501449877991399
7	1.027341023851554	2.5557975058451055
8	0.9821831766492878	2.7925197238119206
9	0.8313149144053532	2.659021523849125
>10	6.160967198981896	32.977338133432546
>50	0.05028942074797815	1.224462998019412
>100	0.027710497146845106	2.1148157469516087
>500	0.002052629418284823	0.543476278537064
>1k	0.00718420296399688	4.2646474152049345
>5k	0.0030789441274272343	6.998077771820207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	7343	2.6783532304011146	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	6115	2.2304412370833195	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	5728	2.0892833043357735	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	2490	0.9082254587632813	RNA PCR Primer, Index 35 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2468	0.9002009767983047	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTTGTCT	2047	0.7466415719230671	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	1300	0.47417393429408267	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCT	1209	0.44098175889349694	RNA PCR Primer, Index 35 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1091	0.39794135562680316	RNA PCR Primer, Index 10 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTA	1087	0.3964823589058984	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTC	970	0.3538067048194309	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	520	0.18966957371763307	TruSeq Adapter, Index 10 (95% over 24bp)
CTGAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	459	0.16741987372383382	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	399	0.14553492291026077	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATG	393	0.14334642782890344	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTTTGTC	366	0.13349819996279558	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTT	280	0.10212977046334089	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGT	278	0.10140027210288846	No Hit
CTGAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCT	276	0.10067077374243602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1965998081419312	0.0
2	0.0	0.0	0.0	1.6920714470694227	0.0
3	0.0	0.0	0.0	2.011226979767363	0.0
4	0.0	0.0	0.0	2.8224291565904704	0.0
5	0.0	0.0	0.0	6.1540481687767405	0.0
6	0.0	0.0	0.0	6.883546529229176	0.0
7	0.0	0.0	0.0	8.16089815838139	0.0
8	0.0	0.0	0.0	9.423295071144327	0.0
9	0.0	0.0	0.0	9.785126257928736	0.0
10	0.0	0.0	0.0	12.695095217773497	0.0
11	0.0	0.0	0.0	13.975000091187296	0.0
12	0.0	0.0	0.0	17.96863886548415	0.0
13	0.0	0.0	0.0	18.559167788270397	0.0
14	0.0	0.0	0.0	18.88087656522992	0.0
15	0.0	0.0	0.0	19.636272117478416	0.0
16	3.6474918022621745E-4	0.0	0.0	20.3887496762851	0.0
17	3.6474918022621745E-4	0.0	0.0	21.17770215311441	0.0
18	3.6474918022621745E-4	0.0	0.0	21.909389008648205	0.0
19	3.6474918022621745E-4	0.0	0.0	23.244006259095933	0.0
20	3.6474918022621745E-4	0.0	0.0	23.857879129416656	0.0
21	3.6474918022621745E-4	0.0	0.0	24.46773975875489	0.0
22	3.6474918022621745E-4	0.0	0.0	25.162586947085835	0.0
23	3.6474918022621745E-4	0.0	0.0	25.754574866592986	0.0
24	3.6474918022621745E-4	0.0	0.0	26.255740240223812	0.0
25	3.6474918022621745E-4	0.0	0.0	26.713135712227487	0.0
26	3.6474918022621745E-4	0.0	0.0	27.111441817034517	0.0
27	3.6474918022621745E-4	0.0	0.0	27.61224244148511	0.0
28	3.6474918022621745E-4	0.0	0.0	28.03389249382662	0.0
29	3.6474918022621745E-4	0.0	0.0	28.42928060519184	0.0
30	3.6474918022621745E-4	0.0	0.0	28.878286846050315	0.0
31	3.6474918022621745E-4	0.0	0.0	29.324375093466976	0.0
32	3.6474918022621745E-4	0.0	0.0	29.80402026546445	0.0
33	3.6474918022621745E-4	0.0	0.0	30.236612793212746	0.0
34	3.6474918022621745E-4	0.0	0.0	30.64039013572317	0.0
35	3.6474918022621745E-4	0.0	0.0	31.08100714543644	0.0
36	3.6474918022621745E-4	0.0	0.0	31.52089465678926	0.0
37	3.6474918022621745E-4	0.0	0.0	31.98084337305452	0.0
38	3.6474918022621745E-4	0.0	0.0	32.41270640244236	0.0
39	3.6474918022621745E-4	0.0	0.0	32.82159023347595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGACGG	45	3.8198777E-10	45.000004	2
AACGAGC	30	2.159435E-6	45.000004	15
GACGTCT	20	7.0238794E-4	45.0	20
CCTCGTA	20	7.0238794E-4	45.0	40
ACGGGTC	40	6.7830115E-9	45.0	5
TCTAGCG	25	3.8830112E-5	45.0	1
CGAATAT	70	0.0	45.0	14
ATTGGGT	20	7.0238794E-4	45.0	4
ACGTAGG	50	2.1827873E-11	45.0	2
CACCGCT	40	6.7830115E-9	45.0	38
CTAGGCG	20	7.0238794E-4	45.0	1
GATGAAT	625	0.0	43.199997	20
CGATGAA	600	0.0	43.125	19
ATGAATG	630	0.0	42.5	21
CGTTTTT	1425	0.0	42.473686	1
TACGGCT	835	0.0	42.30539	7
GACTATG	75	0.0	42.0	24
CCGATGA	615	0.0	41.341465	18
AAGGGCG	115	0.0	41.086956	5
ACGGCTG	860	0.0	41.07558	8
>>END_MODULE