FastQCFastQC Report
Sat 18 Jun 2016
SRR3551922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401173
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC110412.7521792343951366No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC101662.534068843117558No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG88052.194813708798947No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.8966206599147998No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC35770.8916352795427409No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT23160.5773070470844249No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT20310.5062653767825851No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT19390.483332627071114No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA16780.41827341321574485No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC15930.3970855466344943No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC11610.2894013305980213No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT7010.17473758204066575No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATG6160.15354971545941526No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5590.13934138139904728No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTT5520.13759649826882667No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4860.12114474304103218No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4640.11566082463176734No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAACGGAATCGT4090.10195102860860526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG207.027789E-445.0000042
TTTACGG207.027789E-445.0000042
GCGGTAC207.027789E-445.00000421
GACGTTG207.027789E-445.00000428
TTATCGG207.027789E-445.0000042
TATTGCC207.027789E-445.00000412
CGAATAT750.045.00000414
ATGCGTA207.027789E-445.00000420
CGGTCTA207.027789E-445.00000431
AATAGCG207.027789E-445.0000041
CTAGGCG207.027789E-445.0000041
TCAACGG207.027789E-445.0000043
TGCGTAC207.027789E-445.00000421
ACGCAAA253.8862505E-545.013
ATTCGTA351.2092823E-745.032
TTCGTAC351.2092823E-745.033
AAATTCG351.2092823E-745.030
CGTAAGC351.2092823E-745.043
CGATCGA351.2092823E-745.041
CGTTTTT18050.044.3767281