##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551921_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399360
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98935546875	33.0	31.0	34.0	30.0	34.0
2	32.086353165064104	33.0	31.0	34.0	30.0	34.0
3	32.08825871394231	33.0	31.0	34.0	30.0	34.0
4	35.6994866786859	37.0	35.0	37.0	33.0	37.0
5	35.75566907051282	37.0	35.0	37.0	35.0	37.0
6	35.656310096153845	37.0	35.0	37.0	35.0	37.0
7	35.80652544070513	37.0	35.0	37.0	35.0	37.0
8	35.20655799278846	37.0	35.0	37.0	32.0	37.0
9	36.95748447516026	39.0	37.0	39.0	32.0	39.0
10	36.70353315304487	38.0	35.0	39.0	32.0	39.0
11	37.03014823717949	39.0	37.0	39.0	34.0	39.0
12	37.04841496394231	39.0	37.0	39.0	33.0	39.0
13	37.14867037259615	39.0	37.0	39.0	34.0	39.0
14	38.19698267227564	40.0	38.0	41.0	33.0	41.0
15	38.16564002403846	40.0	37.0	41.0	33.0	41.0
16	38.34224759615385	40.0	38.0	41.0	34.0	41.0
17	38.21024389022436	40.0	37.0	41.0	34.0	41.0
18	38.07967497996795	40.0	37.0	41.0	34.0	41.0
19	38.021802383814105	40.0	37.0	41.0	34.0	41.0
20	38.003350360576924	40.0	36.0	41.0	34.0	41.0
21	38.00172526041667	40.0	36.0	41.0	34.0	41.0
22	38.11294320913461	40.0	37.0	41.0	34.0	41.0
23	38.10865885416667	40.0	36.0	41.0	34.0	41.0
24	37.94117838541667	40.0	36.0	41.0	34.0	41.0
25	37.74318159054487	39.0	36.0	41.0	33.0	41.0
26	37.81369441105769	40.0	36.0	41.0	33.0	41.0
27	37.85679336939103	40.0	36.0	41.0	34.0	41.0
28	37.71291065705128	40.0	36.0	41.0	33.0	41.0
29	37.76294070512821	40.0	36.0	41.0	33.0	41.0
30	37.44931139823718	40.0	36.0	41.0	33.0	41.0
31	37.34706029647436	39.0	35.0	41.0	33.0	41.0
32	37.26006610576923	40.0	35.0	41.0	33.0	41.0
33	37.179740084134615	40.0	35.0	41.0	32.0	41.0
34	36.979439603365385	40.0	35.0	41.0	31.0	41.0
35	36.93239433092949	40.0	35.0	41.0	31.0	41.0
36	36.84014172676282	40.0	35.0	41.0	31.0	41.0
37	36.89027944711538	40.0	35.0	41.0	31.0	41.0
38	36.70336288060897	40.0	35.0	41.0	31.0	41.0
39	36.585869891826924	39.0	35.0	41.0	31.0	41.0
40	36.39413311298077	39.0	35.0	41.0	30.0	41.0
41	36.17140174278846	39.0	35.0	41.0	30.0	41.0
42	36.422518529647434	39.0	35.0	41.0	30.0	41.0
43	36.39453125	39.0	35.0	41.0	31.0	41.0
44	36.53089943910256	39.0	35.0	41.0	31.0	41.0
45	36.51065705128205	39.0	35.0	41.0	31.0	41.0
46	36.415965544871796	39.0	35.0	41.0	31.0	41.0
47	36.208804086538464	39.0	35.0	41.0	30.0	41.0
48	36.23096955128205	39.0	35.0	41.0	31.0	41.0
49	36.255686598557695	39.0	35.0	41.0	31.0	41.0
50	36.14088291266026	39.0	35.0	41.0	30.0	41.0
51	35.26847956730769	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	5.0
11	6.0
12	11.0
13	13.0
14	15.0
15	23.0
16	39.0
17	54.0
18	95.0
19	200.0
20	358.0
21	706.0
22	1160.0
23	1773.0
24	2410.0
25	3318.0
26	4268.0
27	4892.0
28	4942.0
29	4934.0
30	5477.0
31	6780.0
32	8773.0
33	12303.0
34	23050.0
35	28740.0
36	28983.0
37	44861.0
38	85487.0
39	125649.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.03210136217949	16.148587740384617	17.76642628205128	18.052884615384617
2	30.847105368589745	31.450070112179485	20.697365785256412	17.00545873397436
3	28.93103966346154	30.792267628205128	25.07061298076923	15.206079727564104
4	26.47911658653846	20.895432692307693	34.37875600961539	18.24669471153846
5	30.477764423076927	24.168419471153847	23.881209935897434	21.472606169871796
6	22.930939503205128	36.46459334935898	25.590194310897434	15.014272836538462
7	68.27599158653847	5.462239583333333	20.269681490384617	5.992087339743589
8	66.08373397435898	12.265374599358974	13.935546874999998	7.715344551282051
9	61.19115584935897	7.003956330128205	15.131961137820513	16.672926682692307
10	40.74293870192307	19.51347155448718	23.213892227564102	16.52969751602564
11	30.846604567307693	22.49774639423077	26.57902644230769	20.076622596153847
12	26.412760416666664	18.875951522435898	30.65079126602564	24.060496794871796
13	25.323768028846157	20.530598958333332	34.74258814102564	19.403044871794872
14	20.810546875	27.626702724358974	28.898988381410255	22.663762019230766
15	18.134014423076923	23.279246794871796	37.32346754807692	21.26327123397436
16	20.09364983974359	23.545172275641026	29.55879407051282	26.802383814102566
17	19.513721955128204	26.078725961538463	31.573768028846157	22.83378405448718
18	19.64092548076923	24.157652243589745	31.50791266025641	24.693509615384617
19	20.887419871794872	25.552884615384613	28.763271233974358	24.796424278846153
20	23.197866586538463	24.817457932692307	33.32532051282051	18.65935496794872
21	22.44140625	28.968599759615383	29.28535657051282	19.304637419871796
22	20.086638621794872	22.252854567307693	32.05378605769231	25.606720753205124
23	21.19641426282051	26.69871794871795	30.328024839743588	21.77684294871795
24	23.202373798076923	23.14953926282051	28.617788461538463	25.0302984775641
25	19.489683493589745	30.503054887820515	26.446063701923077	23.561197916666668
26	18.73973357371795	25.13721955128205	30.880909455128204	25.242137419871796
27	23.253455528846153	25.78625801282051	27.090845352564102	23.869441105769234
28	17.52979767628205	25.53485576923077	31.228465544871796	25.706881009615383
29	21.208934294871796	24.58633814102564	27.969751602564102	26.23497596153846
30	20.769981971153847	26.04992988782051	28.291766826923077	24.888321314102562
31	21.58678886217949	23.92052283653846	25.40840344551282	29.084284855769234
32	23.03535657051282	27.82176482371795	24.07927684294872	25.063601762820515
33	23.13075921474359	24.406800881410255	24.29061498397436	28.171824919871796
34	21.775841346153847	25.713641826923077	28.33708934294872	24.173427483974358
35	20.404146634615383	26.86298076923077	24.99599358974359	27.73687900641026
36	19.930388621794872	31.604316907051285	24.57557091346154	23.889723557692307
37	19.262570112179485	24.43409455128205	28.723207131410255	27.580128205128208
38	21.357171474358974	26.107271634615387	23.33984375	29.195713141025642
39	22.764923878205128	24.040965544871796	27.770933493589745	25.423177083333332
40	23.193609775641026	24.37224559294872	29.51697716346154	22.91716746794872
41	18.65710136217949	28.24944911858974	28.329076522435898	24.764372996794872
42	19.601362179487182	27.090094150641026	29.037710336538463	24.270833333333332
43	22.353265224358974	26.898788060897438	25.38787059294872	25.360076121794872
44	21.925080128205128	22.581129807692307	28.525641025641026	26.968149038461537
45	20.706881009615387	21.817658253205128	26.983673878205128	30.491786858974358
46	25.25340544871795	25.427433894230766	25.871895032051285	23.447265625
47	18.525641025641026	24.180438701923077	33.80709134615385	23.48682892628205
48	20.936247996794872	24.34845753205128	27.384314903846153	27.330979567307693
49	20.150240384615383	21.806891025641026	33.13551682692308	24.90735176282051
50	21.817658253205128	21.416766826923077	30.349559294871796	26.416015625
51	19.425831330128204	21.55724158653846	27.518529647435898	31.498397435897434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	125.0
1	327.5
2	530.0
3	2999.0
4	5468.0
5	3687.0
6	1906.0
7	1867.0
8	1828.0
9	1807.5
10	1787.0
11	1712.0
12	1637.0
13	1539.0
14	1441.0
15	1408.0
16	1375.0
17	1347.0
18	1319.0
19	1212.5
20	1106.0
21	1130.5
22	1155.0
23	1206.5
24	1258.0
25	1561.5
26	2062.0
27	2259.0
28	2739.5
29	3220.0
30	3794.5
31	4369.0
32	4900.0
33	5431.0
34	6375.5
35	7320.0
36	7720.0
37	8120.0
38	9017.0
39	9914.0
40	11149.5
41	12385.0
42	13804.5
43	15224.0
44	17950.0
45	20676.0
46	40225.0
47	59774.0
48	49752.0
49	39730.0
50	40172.0
51	40614.0
52	35193.5
53	29773.0
54	27221.5
55	24670.0
56	21978.0
57	19286.0
58	17935.5
59	16585.0
60	15772.0
61	14959.0
62	13891.5
63	12824.0
64	11034.0
65	9244.0
66	7538.0
67	5832.0
68	5076.5
69	4321.0
70	3894.0
71	3467.0
72	2985.5
73	2504.0
74	2098.0
75	1351.5
76	1011.0
77	883.0
78	755.0
79	577.5
80	400.0
81	262.5
82	125.0
83	78.5
84	32.0
85	23.5
86	15.0
87	19.5
88	24.0
89	13.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.556347984837245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41459139611295	26.103547588448308
2	9.908166913053979	7.045964613047957
3	3.567819937836922	3.805759417709099
4	2.039406735952683	2.900554223446187
5	1.4248434990477283	2.533111563803707
6	1.1276200534192589	2.4056430608433557
7	0.9488299631202055	2.361584984299962
8	0.7983968098570221	2.271045984100817
9	0.7007135532929456	2.2423333444718194
>10	5.982741831200461	34.77257722549276
>50	0.048025307993468096	1.2061212818447096
>100	0.030368944760575417	2.1505772762269606
>500	7.062545293157074E-4	0.20993491393341185
>1k	0.005650036234525659	3.873751175044032
>5k	0.002118763587947122	6.117493347286897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	8975	2.247345753205128	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	8235	2.062049278846154	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	7151	1.790614983974359	No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	3536	0.8854166666666666	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2928	0.7331730769230769	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCGTAAT	1919	0.4805188301282052	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	1611	0.4033954326923077	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	1598	0.40014022435897434	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTA	1377	0.3448016826923077	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTC	1270	0.3180088141025641	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	1187	0.2972255608974359	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	836	0.2093349358974359	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATG	480	0.1201923076923077	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTT	457	0.11443309294871795	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	431	0.1079226762820513	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	405	0.10141225961538462	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.50400641025641E-4	0.0	0.0	0.21459334935897437	0.0
2	2.50400641025641E-4	0.0	0.0	1.6063201121794872	0.0
3	2.50400641025641E-4	0.0	0.0	1.9739082532051282	0.0
4	2.50400641025641E-4	0.0	0.0	2.842297676282051	0.0
5	2.50400641025641E-4	0.0	0.0	6.25175280448718	0.0
6	2.50400641025641E-4	0.0	0.0	7.256860977564102	0.0
7	2.50400641025641E-4	0.0	0.0	8.477313701923077	0.0
8	2.50400641025641E-4	0.0	0.0	10.001752804487179	0.0
9	2.50400641025641E-4	0.0	0.0	10.471254006410257	0.0
10	2.50400641025641E-4	0.0	0.0	13.279246794871796	0.0
11	2.50400641025641E-4	0.0	0.0	14.82772435897436	0.0
12	2.50400641025641E-4	0.0	0.0	18.38115985576923	0.0
13	2.50400641025641E-4	0.0	0.0	19.01817908653846	0.0
14	2.50400641025641E-4	0.0	0.0	19.322916666666668	0.0
15	2.50400641025641E-4	0.0	0.0	20.057091346153847	0.0
16	2.50400641025641E-4	0.0	0.0	20.932491987179485	0.0
17	2.50400641025641E-4	0.0	0.0	21.82567107371795	0.0
18	2.50400641025641E-4	0.0	0.0	22.706330128205128	0.0
19	2.50400641025641E-4	0.0	0.0	23.820863381410255	0.0
20	2.50400641025641E-4	0.0	0.0	24.489182692307693	0.0
21	2.50400641025641E-4	0.0	0.0	25.192057291666668	0.0
22	2.50400641025641E-4	0.0	0.0	25.967798477564102	0.0
23	2.50400641025641E-4	0.0	0.0	26.677183493589745	0.0
24	2.50400641025641E-4	0.0	0.0	27.277393830128204	0.0
25	5.00801282051282E-4	0.0	0.0	27.83027844551282	0.0
26	5.00801282051282E-4	0.0	0.0	28.324569310897434	0.0
27	5.00801282051282E-4	0.0	0.0	28.83513621794872	0.0
28	5.00801282051282E-4	0.0	0.0	29.29962940705128	0.0
29	5.00801282051282E-4	0.0	0.0	29.811949118589745	0.0
30	5.00801282051282E-4	0.0	0.0	30.31700721153846	0.0
31	5.00801282051282E-4	0.0	0.0	30.828826121794872	0.0
32	5.00801282051282E-4	0.0	0.0	31.347405849358974	0.0
33	5.00801282051282E-4	0.0	0.0	31.823167067307693	0.0
34	5.00801282051282E-4	0.0	0.0	32.29792668269231	0.0
35	5.00801282051282E-4	0.0	0.0	32.810496794871796	0.0
36	0.001001602564102564	0.0	0.0	33.308543669871796	0.0
37	0.001001602564102564	0.0	0.0	33.800831330128204	0.0
38	0.001001602564102564	0.0	0.0	34.28135016025641	0.0
39	0.001001602564102564	0.0	0.0	34.74634415064103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACTT	20	7.027751E-4	45.000004	9
CTAACGG	20	7.027751E-4	45.000004	2
CGAATAA	20	7.027751E-4	45.000004	22
GCTAGCG	30	2.1619308E-6	45.000004	1
TTCGGGC	30	2.1619308E-6	45.000004	4
CCGAATA	20	7.027751E-4	45.000004	21
GCGTAAG	20	7.027751E-4	45.000004	1
CCGTAAA	25	3.8862192E-5	45.0	41
CGTAAAC	25	3.8862192E-5	45.0	42
CGTTTTT	1645	0.0	43.35866	1
TACGGCT	1065	0.0	42.676056	7
TACGGGA	80	0.0	42.187504	4
ACGGCTG	1085	0.0	41.8894	8
CCGATGA	485	0.0	41.288662	18
ATGGGCG	60	3.6379788E-12	41.250004	5
GATGAAT	510	0.0	41.02941	20
TAGGGCG	100	0.0	40.5	5
TGATACC	915	0.0	40.081963	4
ATTGGGC	45	1.9228537E-8	40.0	4
GATACCT	920	0.0	39.864132	5
>>END_MODULE