FastQCFastQC Report
Sat 18 Jun 2016
SRR3551916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373732
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC80642.157695889032783No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC76752.0536106086714545No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG65181.7440304817355752No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30820.8246551004463091No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC27600.7384971048772918No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT18450.49366926032558095No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT14590.3903866942086843No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT14400.38530283732728265No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA12390.33152098295034943No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC12310.32938041163186454No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC9600.25686855821818844No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT5870.1570644204938298No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATG4410.1179989939314803No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT4070.10890156582791946No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4060.10863399441310886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT207.02717E-445.045
CTCACGA207.02717E-445.024
CAAACGA253.885736E-545.015
CGCGGTC207.02717E-445.032
ATAGCGG406.7939254E-945.02
CACTAGG253.885736E-545.02
AGCGCGT207.02717E-445.021
CAAGCGC207.02717E-445.025
TTAGTAG253.885736E-545.01
CGTAAGC253.885736E-545.043
ACGTAAG207.02717E-445.01
TCATACT207.02717E-445.040
CACCGCT207.02717E-445.038
TCGCGCG207.02717E-445.029
AGAGCGT207.02717E-445.029
ATCACGG302.161556E-644.9999962
GCATATT600.044.99999627
CGTTTTT17150.043.032071
TACGGCT10250.042.585367
TGATACC7950.042.4528274