##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373732
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.060104566908905	33.0	31.0	34.0	30.0	34.0
2	32.15248894930057	33.0	31.0	34.0	30.0	34.0
3	32.14683784101977	33.0	31.0	34.0	30.0	34.0
4	35.77000899039954	37.0	35.0	37.0	35.0	37.0
5	35.79855886035983	37.0	35.0	37.0	35.0	37.0
6	35.69859417978658	37.0	35.0	37.0	35.0	37.0
7	35.84723545214218	37.0	35.0	37.0	35.0	37.0
8	35.24262038037952	37.0	35.0	37.0	32.0	37.0
9	37.03244570975993	39.0	37.0	39.0	33.0	39.0
10	36.81431614097803	39.0	35.0	39.0	33.0	39.0
11	37.08951601682489	39.0	37.0	39.0	34.0	39.0
12	37.08389166568557	39.0	37.0	39.0	34.0	39.0
13	37.1706731026511	39.0	37.0	39.0	34.0	39.0
14	38.2769926043261	40.0	38.0	41.0	34.0	41.0
15	38.229201673926774	40.0	38.0	41.0	33.0	41.0
16	38.42792161227832	40.0	38.0	41.0	34.0	41.0
17	38.28502509819871	40.0	37.0	41.0	34.0	41.0
18	38.15639014052851	40.0	37.0	41.0	34.0	41.0
19	38.09071473676324	40.0	37.0	41.0	34.0	41.0
20	38.022315455995205	40.0	36.0	41.0	34.0	41.0
21	38.04426166343797	40.0	36.0	41.0	34.0	41.0
22	38.106806481649954	40.0	37.0	41.0	34.0	41.0
23	38.11610726402877	40.0	36.0	41.0	34.0	41.0
24	37.97026746438625	40.0	36.0	41.0	34.0	41.0
25	37.77179101602218	40.0	36.0	41.0	33.0	41.0
26	37.846676227885226	40.0	36.0	41.0	33.0	41.0
27	37.8780008134171	40.0	36.0	41.0	34.0	41.0
28	37.72645371549667	40.0	36.0	41.0	33.0	41.0
29	37.767424250532464	40.0	36.0	41.0	33.0	41.0
30	37.4542292337825	40.0	36.0	41.0	33.0	41.0
31	37.36871608532317	39.0	35.0	41.0	33.0	41.0
32	37.28742521378956	40.0	35.0	41.0	33.0	41.0
33	37.119387689574346	40.0	35.0	41.0	32.0	41.0
34	36.96968683441611	40.0	35.0	41.0	31.0	41.0
35	36.898892789485515	40.0	35.0	41.0	31.0	41.0
36	36.80726563419777	40.0	35.0	41.0	31.0	41.0
37	36.76761957766528	40.0	35.0	41.0	31.0	41.0
38	36.554488242912036	40.0	35.0	41.0	30.0	41.0
39	36.5154683034902	40.0	35.0	41.0	31.0	41.0
40	36.36894352102576	39.0	35.0	41.0	30.0	41.0
41	36.22653131120696	39.0	35.0	41.0	30.0	41.0
42	36.43791540462149	39.0	35.0	41.0	30.0	41.0
43	36.37331028651547	39.0	35.0	41.0	30.0	41.0
44	36.51257853221025	39.0	35.0	41.0	31.0	41.0
45	36.50883520811705	39.0	35.0	41.0	31.0	41.0
46	36.40226151359798	39.0	35.0	41.0	31.0	41.0
47	36.32037930923764	39.0	35.0	41.0	30.0	41.0
48	36.240479809061036	39.0	35.0	41.0	30.0	41.0
49	36.2704852675179	39.0	35.0	41.0	30.0	41.0
50	36.16929243415068	39.0	35.0	41.0	30.0	41.0
51	35.36566577119433	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	9.0
12	8.0
13	10.0
14	15.0
15	27.0
16	34.0
17	54.0
18	85.0
19	170.0
20	471.0
21	916.0
22	1425.0
23	1801.0
24	2274.0
25	2964.0
26	4137.0
27	4729.0
28	4700.0
29	4434.0
30	5081.0
31	6141.0
32	7834.0
33	10872.0
34	21035.0
35	26313.0
36	25998.0
37	39799.0
38	76970.0
39	125378.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.66365202872647	16.197168024145643	19.040114306508407	18.09906564061948
2	30.175098733852064	32.382027763210004	20.803945072939968	16.638928429997968
3	28.58492181563259	30.46915971872893	25.508385688140166	15.437532777498314
4	26.744298053150384	21.750345167125108	34.153885672085885	17.351471107638627
5	30.55612042854238	24.451746171053053	24.308327892714566	20.683805507690003
6	23.16285466591033	36.67146511403894	25.32831012597262	14.837370094078109
7	68.95128059679129	5.688835850288442	19.98410625796025	5.375777294960025
8	67.16042511746384	12.587094495520853	13.30552374428735	6.946956642727945
9	62.3235366519324	7.261085483715604	14.297143407575483	16.118234456776513
10	41.36627315830595	21.417218755685894	21.591942889557224	15.624565196450932
11	32.540965183607504	22.574465124741792	25.836160671283164	19.048409020367536
12	27.95024241970182	18.404899767748013	29.967195744544217	23.67766206800595
13	25.90947523894127	20.48178908950799	34.32165294917213	19.287082722378603
14	20.156422249098284	28.132993696017465	28.118544839617694	23.592039215266556
15	17.59308809521261	24.056275619962967	36.98612909785622	21.3645071869682
16	20.06223711108495	23.066528956578512	30.84349212804898	26.02774180428756
17	19.550908137381867	25.553605257243156	31.502252951312705	23.39323365406227
18	19.62208213372149	24.343112176639945	30.571639570601395	25.463166119037172
19	22.045744009076024	26.014095662132224	27.53684458381942	24.403315744972335
20	25.407778836171374	25.085890424154204	31.680990656406195	17.825340083268223
21	23.769974206115613	28.48779339205634	28.39146768272452	19.350764719103527
22	20.90428435349395	22.606841265933877	31.05861954555671	25.430254835015464
23	22.784508685368124	27.43570258902101	29.197392784134085	20.58239594147678
24	23.846499630751445	23.67204306829493	27.704611860905676	24.77684544004795
25	20.164716962957414	30.789175130842423	26.022657947406163	23.023449958794
26	20.068123682210782	25.932753952029795	30.271692014598695	23.727430351160724
27	24.75196129847056	27.531493155523208	26.516059636316935	21.200485909689295
28	20.480451232433936	27.08571917844873	30.353033724701124	22.080795864416213
29	23.63378035597701	26.488232209176633	27.788094142326585	22.089893292519776
30	21.47768989543309	30.876670983485493	27.036753609538383	20.608885511543033
31	27.273554311645775	25.11719627968705	23.225466376976016	24.38378303169116
32	27.72949600248306	28.66198238309805	23.682745924887353	19.925775689531537
33	24.168655614183425	27.65457600633609	24.084905761347702	24.09186261813278
34	26.93026018644376	26.0159686620359	24.964413001830188	22.089358149690153
35	23.082583241467148	26.42669078377019	24.35943403294339	26.131291941819274
36	23.382798368884654	29.963717316151683	25.728329391114492	20.925154923849178
37	24.731090728115333	27.55370158295249	27.641465007010368	20.073742681921804
38	24.52907430993332	26.44140721158477	23.958879624971903	25.07063885351
39	28.660912097438807	24.16276904305759	26.103196943264155	21.073121916239444
40	22.369237849582053	25.485106975051643	28.096603983603224	24.049051191763084
41	22.260871426583755	25.954694808044266	27.775250714415677	24.009183050956302
42	21.361831472820096	24.429805315038582	29.34295163379106	24.865411578350262
43	24.195412755664485	24.45415431378635	27.913049993043142	23.437382937506023
44	23.211820234820674	24.470476170089796	28.586794815536265	23.73090877955326
45	22.79628182761979	22.27157428317618	26.32153521774962	28.61060867145441
46	26.787377050934897	25.629863110464186	25.51453983068081	22.068220007920115
47	19.790116982222557	25.210043560626332	33.26501343208502	21.73482602506609
48	23.2527586612867	24.155009472028084	27.05039975169373	25.541832114991493
49	21.62993803046033	21.59167531814241	33.0247343015851	23.753652349812164
50	22.1182558624897	22.542624126379334	30.061648453972367	25.277471557158606
51	20.91418449584194	22.144745432555947	27.27783545428275	29.66323461731936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	271.5
2	436.0
3	3048.5
4	5661.0
5	3803.0
6	1945.0
7	1865.5
8	1786.0
9	1751.0
10	1716.0
11	1625.0
12	1534.0
13	1448.0
14	1362.0
15	1261.0
16	1160.0
17	1128.5
18	1097.0
19	1108.5
20	1120.0
21	1073.0
22	1026.0
23	1096.0
24	1166.0
25	1476.0
26	1976.0
27	2166.0
28	2759.0
29	3352.0
30	3858.5
31	4365.0
32	4866.5
33	5368.0
34	5895.5
35	6423.0
36	7140.5
37	7858.0
38	8463.0
39	9068.0
40	10998.5
41	12929.0
42	13915.0
43	14901.0
44	17248.5
45	19596.0
46	36712.0
47	53828.0
48	43942.5
49	34057.0
50	34611.0
51	35165.0
52	31154.5
53	27144.0
54	23919.5
55	20695.0
56	19505.0
57	18315.0
58	18204.5
59	18094.0
60	17263.5
61	16433.0
62	15155.0
63	13877.0
64	11908.0
65	9939.0
66	7951.0
67	5963.0
68	5015.0
69	4067.0
70	3546.0
71	3025.0
72	2603.0
73	2181.0
74	1845.5
75	1112.0
76	714.0
77	556.0
78	398.0
79	323.5
80	249.0
81	175.0
82	101.0
83	64.0
84	27.0
85	16.5
86	6.0
87	8.5
88	11.0
89	5.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.82520367934147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94284157879575	24.67306473352704
2	9.344804290021557	6.321798169071262
3	3.5776436198132746	3.6304357239684157
4	2.0436517072067466	2.765077409836085
5	1.45882931693354	2.467259938933579
6	1.1446728936067156	2.3231276263480996
7	1.0118931735157428	2.3959244887133653
8	0.8889018539115381	2.405382900760161
9	0.7992800194894477	2.4332228510452727
>10	6.682021880419196	37.170713064883074
>50	0.0626414559145172	1.4759603301202433
>100	0.03330305251151547	2.0518074732727354
>500	0.0015858596434054986	0.4149210668173753
>1k	0.005550508751919246	3.501751421052134
>5k	0.002378789465108248	5.969552801651146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	8064	2.157695889032783	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	7675	2.0536106086714545	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	6518	1.7440304817355752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3082	0.8246551004463091	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	2760	0.7384971048772918	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	1845	0.49366926032558095	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT	1459	0.3903866942086843	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT	1440	0.38530283732728265	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTA	1239	0.33152098295034943	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTC	1231	0.32938041163186454	No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	960	0.25686855821818844	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	587	0.1570644204938298	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATG	441	0.1179989939314803	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT	407	0.10890156582791946	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	406	0.10863399441310886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16214827737523144	0.0
2	0.0	0.0	0.0	1.3426733595196558	0.0
3	0.0	0.0	0.0	1.6305802018558753	0.0
4	0.0	0.0	0.0	2.377372020592296	0.0
5	0.0	0.0	0.0	5.5125062879282485	0.0
6	0.0	0.0	0.0	6.410208384617855	0.0
7	0.0	0.0	0.0	7.494675328845269	0.0
8	0.0	0.0	0.0	8.778750548521401	0.0
9	0.0	0.0	0.0	9.168602099900463	0.0
10	0.0	0.0	0.0	11.772607108837349	0.0
11	0.0	0.0	0.0	13.029122472787988	0.0
12	0.0	0.0	0.0	16.275834020099964	0.0
13	0.0	0.0	0.0	16.84067727676517	0.0
14	0.0	0.0	0.0	17.0809564072651	0.0
15	0.0	0.0	0.0	17.73195765949932	0.0
16	0.0	0.0	0.0	18.40382948208877	0.0
17	0.0	0.0	0.0	19.144467158284545	0.0
18	0.0	0.0	0.0	19.850320550554944	0.0
19	0.0	0.0	0.0	20.85210792760588	0.0
20	0.0	0.0	0.0	21.41641604144146	0.0
21	0.0	0.0	0.0	22.00534072543962	0.0
22	0.0	0.0	0.0	22.658750120407138	0.0
23	0.0	0.0	0.0	23.226001519805635	0.0
24	0.0	0.0	0.0	23.723684351353377	0.0
25	0.0	0.0	0.0	24.14832018665782	0.0
26	0.0	0.0	0.0	24.56278830819946	0.0
27	0.0	0.0	0.0	24.98581871501504	0.0
28	0.0	0.0	0.0	25.399751693727055	0.0
29	0.0	0.0	0.0	25.82144424346858	0.0
30	0.0	0.0	0.0	26.263472220735714	0.0
31	0.0	0.0	0.0	26.719413911573	0.0
32	0.0	0.0	0.0	27.192747744372973	0.0
33	0.0	0.0	0.0	27.61390515128488	0.0
34	0.0	0.0	0.0	28.03506255819678	0.0
35	0.0	0.0	0.0	28.458092965012362	0.0
36	0.0	0.0	0.0	28.92420236961245	0.0
37	0.0	0.0	0.0	29.380946774694166	0.0
38	0.0	0.0	0.0	29.802104181606072	0.0
39	0.0	0.0	0.0	30.21202358909593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	20	7.02717E-4	45.0	45
CTCACGA	20	7.02717E-4	45.0	24
CAAACGA	25	3.885736E-5	45.0	15
CGCGGTC	20	7.02717E-4	45.0	32
ATAGCGG	40	6.7939254E-9	45.0	2
CACTAGG	25	3.885736E-5	45.0	2
AGCGCGT	20	7.02717E-4	45.0	21
CAAGCGC	20	7.02717E-4	45.0	25
TTAGTAG	25	3.885736E-5	45.0	1
CGTAAGC	25	3.885736E-5	45.0	43
ACGTAAG	20	7.02717E-4	45.0	1
TCATACT	20	7.02717E-4	45.0	40
CACCGCT	20	7.02717E-4	45.0	38
TCGCGCG	20	7.02717E-4	45.0	29
AGAGCGT	20	7.02717E-4	45.0	29
ATCACGG	30	2.161556E-6	44.999996	2
GCATATT	60	0.0	44.999996	27
CGTTTTT	1715	0.0	43.03207	1
TACGGCT	1025	0.0	42.58536	7
TGATACC	795	0.0	42.452827	4
>>END_MODULE