FastQCFastQC Report
Sat 18 Jun 2016
SRR3551913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544546
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC100951.853837875955383No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC91491.6801151785156807No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG81731.5008833046244028No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40160.7374950876509972No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC38560.708112813242591No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCAGACTT22010.4041899123306387No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCT20100.3691148222556037No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT17910.3288978341590977No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTC16760.30777932442805567No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTA16540.3037392616968998No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC11880.21816338748241654No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT11030.2025540542029507No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9510.17464089351496476No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC6360.11679454077341493No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATG5600.10283796042942193No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5540.10173612513910671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTAC207.030011E-445.0000048
TATTGCG207.030011E-445.0000041
AACGCAC207.030011E-445.00000438
TGCGTCG207.030011E-445.0000041
TCGCAAC253.8880928E-545.016
CGCTAGG453.8380676E-1045.02
TATAGCG253.8880928E-545.01
CTAGGCG253.8880928E-545.01
GCGTACT253.8880928E-545.020
TCGATGG302.1633896E-644.9999962
TACGGCT10350.043.043487
CGTTTTT22150.042.8668171
CGAATAT1600.042.18750414
GACCTAT1450.041.89655333
CGAAAGG700.041.7857172
ATAGCGG700.041.7857172
ACGGCTG10700.041.6355138
TACGAAT1650.040.90909212
TCGTACG501.0786607E-940.530
CGGGAAC451.9250365E-840.06