##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01180616513573	33.0	31.0	34.0	30.0	34.0
2	32.11952341950909	33.0	31.0	34.0	30.0	34.0
3	32.1319466124074	33.0	31.0	34.0	30.0	34.0
4	35.73829575462862	37.0	35.0	37.0	35.0	37.0
5	35.782264491888654	37.0	35.0	37.0	35.0	37.0
6	35.688694802642935	37.0	35.0	37.0	35.0	37.0
7	35.845100322103185	37.0	35.0	37.0	35.0	37.0
8	35.20260363679102	37.0	35.0	37.0	32.0	37.0
9	37.01209080591906	39.0	37.0	39.0	32.0	39.0
10	36.777574346336216	39.0	35.0	39.0	32.0	39.0
11	37.04791881677581	39.0	37.0	39.0	34.0	39.0
12	37.06017489798842	39.0	37.0	39.0	33.0	39.0
13	37.20304253451499	39.0	37.0	39.0	34.0	39.0
14	38.28429737799929	40.0	38.0	41.0	34.0	41.0
15	38.28004429377867	40.0	38.0	41.0	34.0	41.0
16	38.42773245970037	40.0	38.0	41.0	34.0	41.0
17	38.31987013034711	40.0	38.0	41.0	34.0	41.0
18	38.214549367730186	40.0	37.0	41.0	34.0	41.0
19	38.13641455450963	40.0	37.0	41.0	34.0	41.0
20	38.1401461033595	40.0	37.0	41.0	34.0	41.0
21	38.098961336599665	40.0	37.0	41.0	34.0	41.0
22	38.21638759627286	40.0	37.0	41.0	34.0	41.0
23	38.21671080129135	40.0	37.0	41.0	34.0	41.0
24	38.08629390354534	40.0	37.0	41.0	34.0	41.0
25	37.85064622639777	40.0	36.0	41.0	33.0	41.0
26	37.957689524851894	40.0	36.0	41.0	34.0	41.0
27	38.00669915856512	40.0	37.0	41.0	34.0	41.0
28	37.846991806018224	40.0	36.0	41.0	33.0	41.0
29	37.912022124852626	40.0	36.0	41.0	34.0	41.0
30	37.5756869024839	40.0	36.0	41.0	33.0	41.0
31	37.47778149137079	40.0	36.0	41.0	33.0	41.0
32	37.467624406386236	40.0	36.0	41.0	33.0	41.0
33	37.41318272469176	40.0	36.0	41.0	33.0	41.0
34	37.22118425257003	40.0	36.0	41.0	33.0	41.0
35	37.20091782879683	40.0	36.0	41.0	32.0	41.0
36	37.000124874666234	40.0	35.0	41.0	31.0	41.0
37	37.00150951434773	40.0	35.0	41.0	32.0	41.0
38	36.83672637389679	40.0	35.0	41.0	31.0	41.0
39	36.744117117745795	39.0	35.0	41.0	31.0	41.0
40	36.61716365559567	39.0	35.0	41.0	31.0	41.0
41	36.36057927153996	39.0	35.0	41.0	30.0	41.0
42	36.59080591905918	39.0	35.0	41.0	31.0	41.0
43	36.482012538885606	39.0	35.0	41.0	31.0	41.0
44	36.60231091588222	39.0	35.0	41.0	31.0	41.0
45	36.552434505073954	39.0	35.0	41.0	31.0	41.0
46	36.42589974033415	39.0	35.0	41.0	31.0	41.0
47	36.254591898572386	39.0	35.0	41.0	30.0	41.0
48	36.21651798011555	39.0	35.0	41.0	30.0	41.0
49	36.16413489402181	39.0	35.0	41.0	31.0	41.0
50	36.00439265002406	39.0	35.0	40.0	30.0	41.0
51	35.10602777359488	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	12.0
11	9.0
12	8.0
13	13.0
14	25.0
15	32.0
16	36.0
17	57.0
18	128.0
19	248.0
20	494.0
21	1137.0
22	1653.0
23	2227.0
24	2880.0
25	3870.0
26	5179.0
27	6002.0
28	6221.0
29	6384.0
30	7243.0
31	9034.0
32	11751.0
33	16410.0
34	30459.0
35	38389.0
36	39816.0
37	62777.0
38	119812.0
39	172169.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.94883444190206	16.690784616910236	19.637826740073383	16.722554201114324
2	29.794177167769114	31.405244001425043	21.29039603633118	17.510182794474662
3	29.685646391673064	29.606681529200472	24.976402360865748	15.731269718260716
4	27.243428470689345	21.97720670062768	32.46998417029967	18.309380658383315
5	30.457482012538883	25.38426505749744	23.77136183169099	20.386891098272688
6	24.06959191693631	36.39563967047779	24.287571665203675	15.247196747382223
7	70.45869403135823	6.122898708281761	17.947795043944865	5.470612216415143
8	68.66729348852071	12.326782310401692	12.486548427497402	6.519375773580194
9	63.00294190022514	8.35540064567548	13.778633944607066	14.86302350949231
10	40.74641995350255	22.278191374098792	21.495888317974973	15.479500354423687
11	30.387882750033974	22.55438475353781	27.084580549668896	19.97315194675932
12	26.359022010996313	20.031732856361078	30.331505511012846	23.277739621629763
13	25.538705637356625	21.771530779768835	32.989315870468246	19.700447712406298
14	20.359896133659966	27.506767105074687	28.396682741219294	23.736654020046057
15	18.22876304297525	24.505735052686088	35.43998119534438	21.82552070899428
16	20.528476933078196	24.585434472018893	29.510454580512942	25.37563401438997
17	20.222166722370563	26.623646119887024	30.406797589184386	22.747389568558027
18	19.946891539006806	25.326418704755888	30.539568741667374	24.187121014569936
19	21.549510968770317	26.42935583036144	27.50933805408542	24.511795146782823
20	23.595802742100762	26.079339486471298	32.265226445516085	18.05963132591186
21	23.35450081352172	29.438468008212347	28.031424342479788	19.17560683578614
22	21.135404538826837	23.073349175276288	30.918967359965915	24.87227892593096
23	21.591380709802294	27.075031310486164	30.263375362228352	21.07021261748319
24	22.830945411406933	23.948573674216686	29.123343115182188	24.09713779919419
25	20.333635725907452	30.556463549452207	26.283362654394672	22.82653807024567
26	21.156890326987988	25.051143521392135	29.366848714341852	24.425117437278026
27	24.399775225600777	26.43064130486681	27.22947189034535	21.940111579187064
28	19.481733407278725	26.218170733051018	30.722106121429594	23.577989738240664
29	24.677254079545165	23.40702162902675	28.18843587134971	23.727288420078377
30	24.54613567999765	26.506484300683503	27.533394791257304	21.41398522806154
31	23.869608811744094	25.214031505143737	23.958490191829522	26.95786949128265
32	25.03002501166109	29.16906927973027	24.17004256757005	21.63086314103859
33	24.494349421352833	24.485534739030314	24.932879866898297	26.08723597271856
34	23.75446702390615	26.79314511538052	25.499223206120327	23.953164654593
35	25.18262919936975	23.889074568539666	24.695250722620308	26.233045509470276
36	24.586352668094154	25.6382380919151	26.060608286535942	23.714800953454805
37	22.90183014841721	27.398970885838846	27.871841864599133	21.827357101144806
38	22.578625129924745	24.932145310038088	25.178038218993436	27.311191341043735
39	23.580009769606242	27.570489912697916	27.398603607408738	21.4508967102871
40	24.640709875749707	23.324383982253106	28.38713350203656	23.647772639960625
41	20.675021026690125	27.118186526023514	27.21422983549599	24.992562611790373
42	20.761515096979867	23.47276446801556	29.805011881457215	25.960708553547356
43	23.7252683887128	23.688724184917344	28.46389469392852	24.122112732441337
44	23.10768970849111	23.650527228186416	28.23563114961821	25.00615191370426
45	22.723883749031305	22.28259871526005	26.0506917689231	28.94282576678554
46	25.596368350883118	26.000558263213758	25.581493574463863	22.821579811439253
47	20.159913028467752	23.59800641268139	33.479448935443465	22.76263162340739
48	21.840028206983433	24.733631318566292	27.461775497386814	25.964564977063464
49	21.333551251868528	21.86316674808005	32.7874963731255	24.01578562692592
50	22.023300143605866	21.806422230628815	30.702640364634025	25.467637261131294
51	20.727909120625256	22.231730652690498	27.36609946634444	29.674260760339806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	383.5
2	646.0
3	3742.5
4	6839.0
5	4670.5
6	2502.0
7	2380.5
8	2259.0
9	2195.0
10	2131.0
11	2034.0
12	1937.0
13	1844.5
14	1752.0
15	1717.0
16	1682.0
17	1555.0
18	1428.0
19	1346.0
20	1264.0
21	1288.0
22	1312.0
23	1572.0
24	1832.0
25	2238.0
26	3357.0
27	4070.0
28	4701.5
29	5333.0
30	6683.5
31	8034.0
32	8648.5
33	9263.0
34	10221.0
35	11179.0
36	12411.5
37	13644.0
38	14447.5
39	15251.0
40	17352.5
41	19454.0
42	21056.0
43	22658.0
44	24935.5
45	27213.0
46	29353.5
47	31494.0
48	53879.0
49	76264.0
50	63792.0
51	51320.0
52	50632.5
53	49945.0
54	43868.0
55	37791.0
56	33953.0
57	30115.0
58	26637.5
59	23160.0
60	22203.0
61	21246.0
62	19311.0
63	17376.0
64	15177.5
65	12979.0
66	11219.5
67	9460.0
68	8040.0
69	6620.0
70	5686.0
71	4752.0
72	3796.0
73	2840.0
74	2395.5
75	1627.0
76	1303.0
77	994.0
78	685.0
79	608.5
80	532.0
81	359.0
82	186.0
83	115.5
84	45.0
85	35.5
86	26.0
87	16.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.54448331598142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61312324208879	22.905434546313728
2	9.959290768736137	6.283213629868096
3	3.521861851919649	3.332859372872124
4	1.866233608340999	2.354774996881458
5	1.2393279457336044	1.9546979853611608
6	0.8977807010301591	1.6992017007033573
7	0.7770786618159155	1.7158781418000117
8	0.681272759522038	1.719231775710044
9	0.6659330900557271	1.8905863723940555
>10	7.66578076572009	42.988818223354734
>50	0.06259310801538084	1.349649857698413
>100	0.0391938152993506	2.4614409969700746
>500	0.0035098939074045313	0.6875574746765497
>1k	0.005264840861106797	3.5974055203487216
>5k	0.0011699646358015103	3.196422591612237
>10k+	5.849823179007551E-4	1.8628268134352577
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	10095	1.853837875955383	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	9149	1.6801151785156807	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	8173	1.5008833046244028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4016	0.7374950876509972	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	3856	0.708112813242591	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCAGACTT	2201	0.4041899123306387	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCT	2010	0.3691148222556037	No Hit
GAACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	1791	0.3288978341590977	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTC	1676	0.30777932442805567	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCAGACTTCGTA	1654	0.3037392616968998	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1188	0.21816338748241654	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	1103	0.2025540542029507	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	951	0.17464089351496476	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	636	0.11679454077341493	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATG	560	0.10283796042942193	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	554	0.10173612513910671	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21155237574052513	0.0
2	0.0	0.0	0.0	1.3352407326470124	0.0
3	0.0	0.0	0.0	1.6303489512364429	0.0
4	0.0	0.0	0.0	2.358845717349866	0.0
5	0.0	0.0	0.0	5.118024923514267	0.0
6	0.0	0.0	0.0	5.962948951971	0.0
7	0.0	0.0	0.0	7.0524804148777145	0.0
8	0.0	0.0	0.0	8.40094317100851	0.0
9	0.0	0.0	0.0	8.773179859920006	0.0
10	0.0	0.0	0.0	11.090890393098103	0.0
11	0.0	0.0	0.0	12.360204647541254	0.0
12	0.0	0.0	0.0	15.34599464508049	0.0
13	0.0	0.0	0.0	15.888832164775796	0.0
14	0.0	0.0	0.0	16.137479661956935	0.0
15	0.0	0.0	0.0	16.805375487103017	0.0
16	0.0	0.0	0.0	17.521752065022973	0.0
17	0.0	0.0	0.0	18.318746258350995	0.0
18	0.0	0.0	0.0	19.060097769518094	0.0
19	0.0	0.0	0.0	20.003268778027934	0.0
20	0.0	0.0	0.0	20.544820823217872	0.0
21	0.0	0.0	0.0	21.178376115149135	0.0
22	0.0	0.0	0.0	21.86224855200479	0.0
23	0.0	0.0	0.0	22.506271279194046	0.0
24	0.0	0.0	0.0	23.01219731666379	0.0
25	0.0	0.0	0.0	23.520327024714167	0.0
26	0.0	0.0	0.0	23.964917564356362	0.0
27	0.0	0.0	0.0	24.443848637213385	0.0
28	0.0	0.0	0.0	24.889357372930846	0.0
29	0.0	0.0	0.0	25.32164408516452	0.0
30	0.0	0.0	0.0	25.814899016795643	0.0
31	0.0	0.0	0.0	26.28776999555593	0.0
32	0.0	0.0	0.0	26.78102492718705	0.0
33	0.0	0.0	0.0	27.252610431441973	0.0
34	0.0	0.0	0.0	27.72401229648184	0.0
35	0.0	0.0	0.0	28.202208812478652	0.0
36	0.0	0.0	0.0	28.64973757956169	0.0
37	0.0	0.0	0.0	29.130137766139132	0.0
38	0.0	0.0	0.0	29.580788399878063	0.0
39	0.0	0.0	0.0	30.031439033616994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGTAC	20	7.030011E-4	45.000004	8
TATTGCG	20	7.030011E-4	45.000004	1
AACGCAC	20	7.030011E-4	45.000004	38
TGCGTCG	20	7.030011E-4	45.000004	1
TCGCAAC	25	3.8880928E-5	45.0	16
CGCTAGG	45	3.8380676E-10	45.0	2
TATAGCG	25	3.8880928E-5	45.0	1
CTAGGCG	25	3.8880928E-5	45.0	1
GCGTACT	25	3.8880928E-5	45.0	20
TCGATGG	30	2.1633896E-6	44.999996	2
TACGGCT	1035	0.0	43.04348	7
CGTTTTT	2215	0.0	42.866817	1
CGAATAT	160	0.0	42.187504	14
GACCTAT	145	0.0	41.896553	33
CGAAAGG	70	0.0	41.785717	2
ATAGCGG	70	0.0	41.785717	2
ACGGCTG	1070	0.0	41.635513	8
TACGAAT	165	0.0	40.909092	12
TCGTACG	50	1.0786607E-9	40.5	30
CGGGAAC	45	1.9250365E-8	40.0	6
>>END_MODULE