FastQCFastQC Report
Sat 18 Jun 2016
SRR3551912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397596
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC106932.689413374380024No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC97472.4514834153261096No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG84822.1333212607772714No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC40131.0093159890944579TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.9730983209086611No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT24110.6063944305274701No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT19590.49271119427760846No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT17910.4504572480608457No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA16540.4160001609674142No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC15580.39185504884354977No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC14040.3531222648115172TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT10290.2588054205776718TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG6050.1521645086972706No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT4810.12097707220394571No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC4290.10789846980351915No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC4250.1068924234650248TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT4080.1026167265264238TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCATGCAC4070.10236521494180022No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT4030.10135916860330586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG453.8380676E-1045.0000042
CTATGCG253.8861865E-545.01
TTTACGG406.7957444E-945.02
CACGAAT207.0277136E-445.044
CTACGCG207.0277136E-445.01
TGTTAAC207.0277136E-445.044
TTACGAG253.8861865E-545.01
AATAGCG207.0277136E-445.01
CAATCAC406.7957444E-945.042
CGTTTTT20250.043.111111
TACGGCT11000.042.9545447
CTCGAAT700.041.78571743
AACGGGC650.041.538464
CCGATGA4900.041.3265318
CGATGAA4900.041.3265319
CGAATAT603.6379788E-1241.24999614
ACGGCTG11600.040.9267238
TACGAAT556.002665E-1140.90909212
TGATACC10100.040.7673264
TATGCGG501.0786607E-940.52
Produced by FastQC (version 0.11.5)