##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397596
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01556102174066	33.0	31.0	34.0	30.0	34.0
2	32.110687733277	33.0	31.0	34.0	30.0	34.0
3	32.11177174820672	33.0	31.0	34.0	30.0	34.0
4	35.729157234982246	37.0	35.0	37.0	35.0	37.0
5	35.765088179961566	37.0	35.0	37.0	35.0	37.0
6	35.671525870481595	37.0	35.0	37.0	35.0	37.0
7	35.809319007233476	37.0	35.0	37.0	35.0	37.0
8	35.16944586967676	37.0	35.0	37.0	32.0	37.0
9	36.91506705298846	39.0	37.0	39.0	32.0	39.0
10	36.71243674483647	38.0	35.0	39.0	32.0	39.0
11	37.04556383867041	39.0	37.0	39.0	34.0	39.0
12	37.049912473968554	39.0	37.0	39.0	33.0	39.0
13	37.14271018823127	39.0	37.0	39.0	33.0	39.0
14	38.20475055081037	40.0	37.0	41.0	33.0	41.0
15	38.14326602883329	40.0	37.0	41.0	33.0	41.0
16	38.350141852533724	40.0	38.0	41.0	34.0	41.0
17	38.217756214851256	40.0	37.0	41.0	34.0	41.0
18	38.058999587521	40.0	37.0	41.0	34.0	41.0
19	38.006290304731436	40.0	37.0	41.0	34.0	41.0
20	37.93037153291281	40.0	36.0	41.0	34.0	41.0
21	37.965198342035634	40.0	36.0	41.0	34.0	41.0
22	38.06411784826809	40.0	36.0	41.0	34.0	41.0
23	38.066331150212775	40.0	36.0	41.0	34.0	41.0
24	37.892101530196484	40.0	36.0	41.0	33.0	41.0
25	37.7156208814978	39.0	36.0	41.0	33.0	41.0
26	37.807452791275566	40.0	36.0	41.0	33.0	41.0
27	37.831809173130516	40.0	36.0	41.0	34.0	41.0
28	37.66238342438053	40.0	36.0	41.0	33.0	41.0
29	37.77266622400628	40.0	36.0	41.0	33.0	41.0
30	37.418387005905494	40.0	35.0	41.0	33.0	41.0
31	37.26088039115081	39.0	35.0	41.0	33.0	41.0
32	37.20585468666687	40.0	35.0	41.0	33.0	41.0
33	37.107226933872575	40.0	35.0	41.0	32.0	41.0
34	36.932549623235644	40.0	35.0	41.0	31.0	41.0
35	36.83481976679846	40.0	35.0	41.0	31.0	41.0
36	36.70552520649101	40.0	35.0	41.0	31.0	41.0
37	36.72994949647381	40.0	35.0	41.0	31.0	41.0
38	36.46410929687421	39.0	35.0	41.0	30.0	41.0
39	36.396266562037844	39.0	35.0	41.0	30.0	41.0
40	36.278815682250325	39.0	35.0	41.0	30.0	41.0
41	36.1881281501826	39.0	35.0	41.0	29.0	41.0
42	36.321124961015705	39.0	35.0	41.0	30.0	41.0
43	36.28267890019014	39.0	35.0	41.0	30.0	41.0
44	36.40678729162265	39.0	35.0	41.0	30.0	41.0
45	36.400197688105514	39.0	35.0	41.0	31.0	41.0
46	36.298126741717724	39.0	35.0	41.0	30.0	41.0
47	36.16378434390688	39.0	35.0	41.0	30.0	41.0
48	36.17794947635288	39.0	35.0	41.0	30.0	41.0
49	36.22967031861487	39.0	35.0	41.0	30.0	41.0
50	36.12020493163915	39.0	35.0	41.0	30.0	41.0
51	35.2936322297005	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	8.0
11	4.0
12	8.0
13	13.0
14	14.0
15	20.0
16	33.0
17	59.0
18	81.0
19	169.0
20	394.0
21	855.0
22	1480.0
23	2000.0
24	2606.0
25	3363.0
26	4483.0
27	5126.0
28	5159.0
29	5137.0
30	5525.0
31	6677.0
32	8727.0
33	12089.0
34	22896.0
35	28756.0
36	28262.0
37	43174.0
38	81285.0
39	129128.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.887554200746486	15.556494532138151	17.789414380426365	18.766536886689
2	30.230686425416753	32.20178271411181	20.121429793056265	17.446101067415164
3	27.650177567178748	31.573758287306713	26.018118894556284	14.75794525095826
4	25.44014527309128	20.626465054980432	36.67516775822695	17.258221913701345
5	31.36450064889989	23.367689815792918	24.709252608175134	20.558556927132063
6	22.768337709634906	36.38240827372508	26.116962947313354	14.732291069326653
7	65.80222135031539	5.312176178834798	22.884536061731005	6.001066409118804
8	63.304208291833916	13.398273624483142	15.60050905944728	7.697009024235656
9	58.680419320113884	6.857463354762121	16.41943077898168	18.042686546142313
10	40.18023320154126	19.326653185645732	23.94415436775018	16.54895924506283
11	31.19347277135585	21.158160544874697	27.294288674936368	20.35407800883309
12	25.83501846095031	17.153593094497932	32.30213583637662	24.709252608175134
13	24.33776999768609	20.314590690047183	36.94956689705128	18.398072415215445
14	20.36615056489502	27.45978329761869	29.310908560448297	22.863157577038
15	16.594985865048944	22.724071670741157	39.42896809827061	21.251974365939294
16	18.672220042455155	23.284690992867134	30.52747009527259	27.515618869405124
17	18.86261431201521	25.173542993390274	32.75561122345295	23.20823147114156
18	18.161148502500023	23.909194257487503	32.55767160635419	25.371985633658284
19	19.464984557188707	25.18083682934436	29.591092465718972	25.763086147747966
20	22.33598929566696	24.619714484049133	34.75261320536424	18.29168301491967
21	21.639553717844244	29.386362035835372	30.0893369148583	18.884747331462087
22	18.83218141027576	22.184327810138935	32.935442006458814	26.048048773126492
23	20.67400074447429	26.696194126701478	31.26993229308142	21.359872835742813
24	22.333725691405345	22.513053451241966	29.422076680851923	25.73114417650077
25	18.681274459501605	30.352669543959195	27.29705530236723	23.669000694171974
26	18.03237457117275	22.83473676797553	33.385899254519664	25.746989406332055
27	22.763307477942433	25.60211873358887	28.207778750289236	23.42679503817946
28	17.052233925894626	25.67430255837584	33.69928268896065	23.57418082676888
29	20.752723870461473	20.51479391140756	34.04259600197185	24.689886216159117
30	20.20166198855119	25.928077747261042	30.13133934949044	23.73892091469733
31	21.04749544764032	25.39864586162839	26.59533798126742	26.95852070946388
32	22.677290516001168	27.362196802784737	29.276451473354864	20.684061207859234
33	23.119699393354058	22.522107868288412	25.150152416020283	29.208040322337247
34	21.44085956599161	22.86340908862262	29.8760550910975	25.81967625428827
35	20.364389983802653	24.159196772603348	27.160987535085866	28.315425708508137
36	19.60633406774716	26.712793891286633	27.6539502409481	26.02692180001811
37	18.19862372860894	24.220817110836126	31.560428173321665	26.02013098723327
38	21.504491996901375	27.529452006559424	24.995724303061397	25.9703316934778
39	22.332971156651478	21.668477550075956	30.3783237255908	25.62022756768177
40	23.327447962253142	23.086499864183743	29.63284338876649	23.95320878479663
41	18.247919999195165	25.07721405647944	25.24924798036198	31.425617963963422
42	18.62845702673065	25.58375838791135	30.793820863388966	24.993963721969035
43	22.09051398907434	22.147355607199266	27.443435044618152	28.318695359108244
44	21.029889636716668	21.39936015452872	30.208804917554506	27.36194529120011
45	20.05075503777704	20.929536514451854	28.358182677894145	30.661525769876963
46	25.223845310314992	23.85864042897816	27.438907836094927	23.478606424611918
47	17.838710651012583	22.507017173210997	36.44201651928088	23.21225565649554
48	20.347035684463627	23.277648668497672	28.16074608396463	28.214569563074075
49	19.54748035694524	20.395325908711357	35.35649252004547	24.70070121429793
50	21.270083200032193	19.373434340385717	31.68291431503335	27.67356814454874
51	19.12619845270073	19.706435678427347	28.521916719484096	32.64544914938782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	280.5
2	459.0
3	3659.5
4	6860.0
5	4525.0
6	2190.0
7	2137.0
8	2084.0
9	2018.5
10	1953.0
11	1897.0
12	1841.0
13	1753.5
14	1666.0
15	1551.5
16	1437.0
17	1379.0
18	1321.0
19	1294.0
20	1267.0
21	1283.0
22	1299.0
23	1398.0
24	1497.0
25	1560.0
26	1948.0
27	2273.0
28	2708.0
29	3143.0
30	3573.0
31	4003.0
32	4717.5
33	5432.0
34	6128.0
35	6824.0
36	7620.0
37	8416.0
38	8983.5
39	9551.0
40	10714.0
41	11877.0
42	13662.0
43	15447.0
44	17860.5
45	20274.0
46	42737.5
47	65201.0
48	53309.0
49	41417.0
50	41252.0
51	41087.0
52	34954.0
53	28821.0
54	25452.5
55	22084.0
56	19957.0
57	17830.0
58	16665.5
59	15501.0
60	14695.0
61	13889.0
62	12452.0
63	11015.0
64	9997.0
65	8979.0
66	7430.5
67	5882.0
68	5129.0
69	4376.0
70	3770.0
71	3164.0
72	2640.0
73	2116.0
74	1814.5
75	1194.5
76	876.0
77	662.5
78	449.0
79	371.0
80	293.0
81	217.5
82	142.0
83	108.5
84	75.0
85	51.5
86	28.0
87	19.5
88	11.0
89	8.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.47114799719034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90387811850928	25.85984250310769
2	10.358216254276122	7.348356434846619
3	3.803776427136806	4.047729497855808
4	2.1062477381674407	2.9884410095713876
5	1.3814803030735305	2.4501346142762284
6	1.1598985144961724	2.4685759121648974
7	0.9572340439958988	2.376793330976928
8	0.8350056122151774	2.369488611949527
9	0.7651228250191657	2.442580646605488
>10	5.638134608613673	31.72408164524945
>50	0.05332356708793698	1.3406584415712703
>100	0.028439235780233052	2.171634657706962
>500	7.109808945058263E-4	0.1525768166197552
>1k	0.006398828050552438	4.965177463817752
>5k	0.0014219617890116527	4.597227752333087
>10k+	7.109808945058263E-4	2.6967006613471773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	10693	2.689413374380024	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	9747	2.4514834153261096	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	8482	2.1333212607772714	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	4013	1.0093159890944579	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3869	0.9730983209086611	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	2411	0.6063944305274701	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	1959	0.49271119427760846	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	1791	0.4504572480608457	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	1654	0.4160001609674142	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	1558	0.39185504884354977	No Hit
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	1404	0.3531222648115172	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1029	0.2588054205776718	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATG	605	0.1521645086972706	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	481	0.12097707220394571	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	429	0.10789846980351915	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	425	0.1068924234650248	TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCTT	408	0.1026167265264238	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCATGCAC	407	0.10236521494180022	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCATGCACTCGT	403	0.10135916860330586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26383565227014355	0.0
2	0.0	0.0	0.0	1.8561554945220777	0.0
3	0.0	0.0	0.0	2.2668739122123966	0.0
4	0.0	0.0	0.0	3.2012394490890252	0.0
5	0.0	0.0	0.0	6.993279610458858	0.0
6	0.0	0.0	0.0	8.049628265877926	0.0
7	0.0	0.0	0.0	9.443505467861849	0.0
8	0.0	0.0	0.0	11.12033320254731	0.0
9	0.0	0.0	0.0	11.631656254087064	0.0
10	0.0	0.0	0.0	14.820068612360286	0.0
11	0.0	0.0	0.0	16.42446101067415	0.0
12	2.5151158462358777E-4	0.0	0.0	20.53164518757734	0.0
13	2.5151158462358777E-4	0.0	0.0	21.252728900693167	0.0
14	2.5151158462358777E-4	0.0	0.0	21.56208814978018	0.0
15	2.5151158462358777E-4	0.0	0.0	22.421754746023602	0.0
16	2.5151158462358777E-4	0.0	0.0	23.317890522037445	0.0
17	2.5151158462358777E-4	0.0	0.0	24.234147224821175	0.0
18	2.5151158462358777E-4	0.0	0.0	25.121480095373194	0.0
19	2.5151158462358777E-4	0.0	0.0	26.391110574553064	0.0
20	2.5151158462358777E-4	0.0	0.0	27.110433706576526	0.0
21	2.5151158462358777E-4	0.0	0.0	27.855411020231593	0.0
22	2.5151158462358777E-4	0.0	0.0	28.680620529381585	0.0
23	2.5151158462358777E-4	0.0	0.0	29.43943098019095	0.0
24	2.5151158462358777E-4	0.0	0.0	30.01237436996348	0.0
25	2.5151158462358777E-4	0.0	0.0	30.554633346411936	0.0
26	2.5151158462358777E-4	0.0	0.0	31.088089417398564	0.0
27	2.5151158462358777E-4	0.0	0.0	31.63588164870874	0.0
28	2.5151158462358777E-4	0.0	0.0	32.13613819052505	0.0
29	2.5151158462358777E-4	0.0	0.0	32.642431010372334	0.0
30	2.5151158462358777E-4	0.0	0.0	33.19072626485176	0.0
31	2.5151158462358777E-4	0.0	0.0	33.710600710268714	0.0
32	2.5151158462358777E-4	0.0	0.0	34.239781084316746	0.0
33	2.5151158462358777E-4	0.0	0.0	34.73601344077908	0.0
34	2.5151158462358777E-4	0.0	0.0	35.221179287517984	0.0
35	2.5151158462358777E-4	0.0	0.0	35.74331733719655	0.0
36	2.5151158462358777E-4	0.0	0.0	36.22848318393545	0.0
37	2.5151158462358777E-4	0.0	0.0	36.74986669886015	0.0
38	2.5151158462358777E-4	0.0	0.0	37.21616917675228	0.0
39	2.5151158462358777E-4	0.0	0.0	37.716425718568594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	45	3.8380676E-10	45.000004	2
CTATGCG	25	3.8861865E-5	45.0	1
TTTACGG	40	6.7957444E-9	45.0	2
CACGAAT	20	7.0277136E-4	45.0	44
CTACGCG	20	7.0277136E-4	45.0	1
TGTTAAC	20	7.0277136E-4	45.0	44
TTACGAG	25	3.8861865E-5	45.0	1
AATAGCG	20	7.0277136E-4	45.0	1
CAATCAC	40	6.7957444E-9	45.0	42
CGTTTTT	2025	0.0	43.11111	1
TACGGCT	1100	0.0	42.954544	7
CTCGAAT	70	0.0	41.785717	43
AACGGGC	65	0.0	41.53846	4
CCGATGA	490	0.0	41.32653	18
CGATGAA	490	0.0	41.32653	19
CGAATAT	60	3.6379788E-12	41.249996	14
ACGGCTG	1160	0.0	40.926723	8
TACGAAT	55	6.002665E-11	40.909092	12
TGATACC	1010	0.0	40.767326	4
TATGCGG	50	1.0786607E-9	40.5	2
>>END_MODULE