SRR3551911_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237767
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	6855	2.8830746066527317	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	6190	2.60338903211968	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	5905	2.4835237858912294	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2706	1.1380889694532883	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2695	1.1334625915286813	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT	1846	0.7763903317112972	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT	1418	0.5963821724629574	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA	1278	0.5375009988770518	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1254	0.5274070834051824	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	1219	0.512686790008706	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC	1090	0.45843199434740733	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	747	0.3141731190619388	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATG	414	0.1741200418897492	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGT	348	0.14636177434210804	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAGTGTG	346	0.14552061471945224	No Hit
TGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT	300	0.126173943398369	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTAT	289	0.12154756547376212	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	282	0.11860350679446685	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	264	0.11103307019056471	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT	261	0.10977133075658102	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	245	0.10304205377533468	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGG	20	7.0219894E-4	45.000004	1
AAGGTTG	30	2.1582164E-6	45.000004	9
ATAGAGT	20	7.0219894E-4	45.000004	17
CATTCTA	30	2.1582164E-6	45.000004	45
TACGGGA	40	6.7757355E-9	45.000004	4
CCGTCAC	20	7.0219894E-4	45.000004	11
CTACGAA	40	6.7757355E-9	45.000004	11
TACTGAA	40	6.7757355E-9	45.000004	3
TTTGGGC	20	7.0219894E-4	45.000004	4
CTTTGCG	20	7.0219894E-4	45.000004	1
CGAATAT	40	6.7757355E-9	45.000004	14
CCGACTT	20	7.0219894E-4	45.000004	44
GTTCAAT	20	7.0219894E-4	45.000004	12
CTCGAGT	30	2.1582164E-6	45.000004	13
GCTACGA	40	6.7757355E-9	45.000004	10
CGGTAGT	20	7.0219894E-4	45.000004	12
AGCTACG	40	6.7757355E-9	45.000004	9
TTCGCAG	20	7.0219894E-4	45.000004	1
TAAGCCG	20	7.0219894E-4	45.000004	45
CTCTACA	20	7.0219894E-4	45.000004	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content