##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551911_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237767
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03092102772883	33.0	31.0	34.0	30.0	34.0
2	32.14264805460808	33.0	31.0	34.0	30.0	34.0
3	32.14038533522314	33.0	31.0	34.0	30.0	34.0
4	35.76525758410545	37.0	35.0	37.0	35.0	37.0
5	35.79704500624561	37.0	35.0	37.0	35.0	37.0
6	35.69600070657408	37.0	35.0	37.0	35.0	37.0
7	35.81261907665908	37.0	35.0	37.0	35.0	37.0
8	35.09201865692043	37.0	35.0	37.0	32.0	37.0
9	36.80498555308348	39.0	37.0	39.0	32.0	39.0
10	36.758456808556275	38.0	35.0	39.0	32.0	39.0
11	37.10865258845845	39.0	37.0	39.0	34.0	39.0
12	37.0608747218916	39.0	37.0	39.0	34.0	39.0
13	37.133412121951324	39.0	37.0	39.0	33.0	39.0
14	38.18053808981061	40.0	37.0	41.0	33.0	41.0
15	38.137538009900446	40.0	37.0	41.0	33.0	41.0
16	38.36532824151375	40.0	37.0	41.0	34.0	41.0
17	38.2272182430699	40.0	37.0	41.0	34.0	41.0
18	38.08200885741083	40.0	37.0	41.0	34.0	41.0
19	37.991453818233815	40.0	37.0	41.0	34.0	41.0
20	37.8686529249223	40.0	36.0	41.0	33.0	41.0
21	37.92343765114587	40.0	36.0	41.0	34.0	41.0
22	38.01072478518886	40.0	36.0	41.0	34.0	41.0
23	37.99982756227735	40.0	36.0	41.0	34.0	41.0
24	37.87024692240723	40.0	35.0	41.0	34.0	41.0
25	37.67736481513414	39.0	35.0	41.0	33.0	41.0
26	37.76816799639984	40.0	35.0	41.0	33.0	41.0
27	37.7602064205714	40.0	35.0	41.0	33.0	41.0
28	37.60756959544428	40.0	35.0	41.0	33.0	41.0
29	37.71508241261403	40.0	36.0	41.0	33.0	41.0
30	37.316347516686506	39.0	35.0	41.0	33.0	41.0
31	37.19220917957496	39.0	35.0	41.0	33.0	41.0
32	37.087135725310915	39.0	35.0	41.0	32.0	41.0
33	36.84581123536908	39.0	35.0	41.0	31.0	41.0
34	36.680266815832304	39.0	35.0	41.0	31.0	41.0
35	36.58418115213634	40.0	35.0	41.0	31.0	41.0
36	36.4338827507602	39.0	35.0	41.0	30.0	41.0
37	36.49327282591781	39.0	35.0	41.0	30.0	41.0
38	36.14194568632316	39.0	35.0	41.0	30.0	41.0
39	36.16877447248777	39.0	35.0	41.0	30.0	41.0
40	35.9993354838981	39.0	35.0	41.0	29.0	41.0
41	35.861372688388215	39.0	35.0	41.0	28.0	41.0
42	36.03567357959683	39.0	35.0	41.0	30.0	41.0
43	35.983471213414816	39.0	35.0	41.0	30.0	41.0
44	36.10323131469043	39.0	35.0	41.0	30.0	41.0
45	36.10367712929044	39.0	35.0	41.0	30.0	41.0
46	36.061042953816134	39.0	35.0	41.0	30.0	41.0
47	35.888441205045275	39.0	35.0	41.0	29.0	41.0
48	35.8962724011322	39.0	35.0	41.0	29.0	41.0
49	35.936076074476276	39.0	35.0	41.0	29.0	41.0
50	35.81693001972519	39.0	35.0	41.0	29.0	41.0
51	34.90815798660033	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	7.0
12	7.0
13	4.0
14	6.0
15	8.0
16	16.0
17	27.0
18	43.0
19	127.0
20	333.0
21	656.0
22	949.0
23	1150.0
24	1535.0
25	2133.0
26	2792.0
27	3261.0
28	3218.0
29	3136.0
30	3504.0
31	4243.0
32	5526.0
33	7427.0
34	14074.0
35	18498.0
36	16869.0
37	25892.0
38	49641.0
39	72654.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.08362808968444	14.451542897037855	17.279521548406635	20.185307464871073
2	29.77789180163774	33.16818566075192	19.992681911282894	17.061240626327457
3	26.651301484226153	32.710174246215836	26.14870860968932	14.489815659868697
4	25.554008756471674	19.741175184108812	38.1507946855535	16.554021373866014
5	31.149402566378008	22.36475204717223	25.731493436852045	20.754351949597716
6	21.522751264893785	36.68212998439649	26.888087918003762	14.90703083270597
7	62.967106452956045	5.712314997455492	24.55429054494526	6.766288004643201
8	59.84640425290305	15.039092893462927	16.75253504481278	8.361967808821241
9	56.17390134038786	7.200746949744918	16.76431127952996	19.861040430337262
10	40.7710069101263	17.957496204267205	25.147728658728923	16.123768226877573
11	32.61217915017644	20.505789281102928	26.6685452564906	20.21348631223004
12	25.64064819760522	17.085634255384473	31.72433516846324	25.549382378547065
13	24.422228484188302	21.101750873754558	36.73344072137849	17.742579920678647
14	19.710472857881875	28.912759129736255	28.476617865389226	22.900150146992644
15	15.648933619888378	22.475364537551467	40.97540869843166	20.900293144128497
16	17.775385145962225	22.956087261899256	31.160337641472534	28.10818995066599
17	18.081146668797604	25.109876475709413	33.03906765867425	23.769909196818734
18	17.517569721618223	23.425874911152516	33.06388186754259	25.99267349968667
19	19.911510007696613	24.637565347588186	29.118843237286924	26.33208140742828
20	22.502281645476454	24.2624081558837	34.821484899081874	18.41382529955797
21	21.12530334318892	29.900280526734157	29.467924480689078	19.506491649387847
22	18.446630524841545	22.41900684283353	32.491893324136655	26.642469308188264
23	19.758418956373255	26.821215728002624	31.84083577620107	21.57952953942305
24	22.919917398125055	22.05983168395951	29.53269377163357	25.487557146281866
25	17.651314101620493	31.322681448645103	27.575315329713547	23.450689120020858
26	17.387610559917903	22.36475204717223	34.11869603435296	26.128941358556908
27	22.74327387736734	25.6343395004353	28.297871445574867	23.324515176622494
28	16.585564859715603	26.173943398368994	33.4785735615119	23.761918180403505
29	21.312881939041162	20.511256818650192	33.23547843056438	24.94038281174427
30	19.159933884853658	25.914866234591006	31.571664696951217	23.353535183604116
31	24.12109333927753	24.989170069858307	25.77817779590944	25.111558794954725
32	26.907014009513514	25.52793280816934	27.46512341914563	20.09992976317151
33	23.104551935298	21.953004411882223	27.788969873868112	27.153473778951664
34	26.11632396421707	21.959733688863466	30.084494484095774	21.83944786282369
35	22.06109342339349	19.837908540714228	30.91471903165704	27.186279004235235
36	24.81799408664785	25.053518780991475	29.57937813069097	20.549109001669702
37	23.46456825379468	22.6944866192533	31.11870024015107	22.722244886800944
38	21.816315973200652	26.235768630634194	27.92649947217234	24.021415923992816
39	30.34104816900579	21.40751239658994	28.255392884630755	19.99604654977352
40	22.260027674151587	19.286948987874684	36.36290990759862	22.090113430375116
41	25.599431376095083	22.74832083510327	26.501575071393425	25.150672717408217
42	22.01272674509078	20.38382113581784	35.11462902757742	22.48882309151396
43	27.775090740094292	20.362371565440114	27.529051550467475	24.333486143998115
44	22.995621764164078	20.662244970916905	30.560170250707625	25.78196301421139
45	21.04539317903662	20.155446298266792	29.304318934082524	29.49484158861406
46	28.600268329919626	22.48882309151396	28.172959241610485	20.73794933695593
47	18.776365096922618	20.77538094016411	38.94232589047261	21.505928072440668
48	23.423351432284548	21.524854163950423	29.228614568043504	25.823179835721525
49	21.57153852300782	17.8595011082278	37.35758116138909	23.211379207375288
50	23.949496776255746	18.735989435035137	32.03219959035526	25.282314198353852
51	21.134135519226806	19.003478195039683	28.526666862937244	31.335719422796267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	185.5
2	317.0
3	2407.5
4	4498.0
5	2971.5
6	1445.0
7	1422.0
8	1399.0
9	1374.0
10	1349.0
11	1341.0
12	1333.0
13	1237.5
14	1142.0
15	1084.5
16	1027.0
17	881.0
18	735.0
19	761.0
20	787.0
21	748.0
22	709.0
23	715.5
24	722.0
25	790.5
26	1049.0
27	1239.0
28	1465.0
29	1691.0
30	2056.5
31	2422.0
32	2705.0
33	2988.0
34	3287.5
35	3587.0
36	3892.0
37	4197.0
38	4501.5
39	4806.0
40	5778.0
41	6750.0
42	8075.5
43	9401.0
44	11141.0
45	12881.0
46	27630.5
47	42380.0
48	33489.5
49	24599.0
50	24363.0
51	24127.0
52	20138.0
53	16149.0
54	14298.5
55	12448.0
56	11349.5
57	10251.0
58	9298.5
59	8346.0
60	8098.0
61	7850.0
62	7290.0
63	6730.0
64	5984.0
65	5238.0
66	4524.5
67	3811.0
68	3347.0
69	2883.0
70	2554.0
71	2225.0
72	1947.0
73	1669.0
74	1401.5
75	924.5
76	715.0
77	597.5
78	480.0
79	354.5
80	229.0
81	168.5
82	108.0
83	71.5
84	35.0
85	25.5
86	16.0
87	10.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	237767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.45487809494169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06405693950178	26.259321100068554
2	10.05693950177936	7.1313512808758155
3	3.767497034400949	4.0072844423322
4	2.01423487544484	2.856578078539074
5	1.3226571767497035	2.3447324481530236
6	0.9584816132858838	2.038970925317643
7	0.8659549228944247	2.149162835885552
8	0.7034400948991697	1.9952306249395417
9	0.6666666666666667	2.127292685696501
>10	5.479240806642942	31.69489458167029
>50	0.05575326215895611	1.2907594409653147
>100	0.03084223013048636	2.139910080036338
>500	0.0011862396204033213	0.3141731190619388
>1k	0.00948991696322657	5.680350931794572
>5k	0.0035587188612099642	7.969987424663641
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	6855	2.8830746066527317	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	6190	2.60338903211968	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	5905	2.4835237858912294	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	2706	1.1380889694532883	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2695	1.1334625915286813	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT	1846	0.7763903317112972	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT	1418	0.5963821724629574	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA	1278	0.5375009988770518	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1254	0.5274070834051824	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	1219	0.512686790008706	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC	1090	0.45843199434740733	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	747	0.3141731190619388	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATG	414	0.1741200418897492	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGT	348	0.14636177434210804	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAGTGTG	346	0.14552061471945224	No Hit
TGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCTT	300	0.126173943398369	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTAT	289	0.12154756547376212	No Hit
TCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	282	0.11860350679446685	TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	264	0.11103307019056471	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT	261	0.10977133075658102	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	245	0.10304205377533468	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3221641354771688	0.0
2	0.0	0.0	0.0	2.250101990604247	0.0
3	0.0	0.0	0.0	2.7312452947633608	0.0
4	0.0	0.0	0.0	3.7709185883659213	0.0
5	0.0	0.0	0.0	7.782829408622727	0.0
6	0.0	0.0	0.0	8.743854277506971	0.0
7	0.0	0.0	0.0	10.238594926966316	0.0
8	0.0	0.0	0.0	12.014703470204024	0.0
9	0.0	0.0	0.0	12.5013143119104	0.0
10	0.0	0.0	0.0	16.051428499329177	0.0
11	0.0	0.0	0.0	17.72575672822553	0.0
12	0.0	0.0	0.0	22.078337195657934	0.0
13	0.0	0.0	0.0	22.90267362586061	0.0
14	0.0	0.0	0.0	23.278671977187752	0.0
15	0.0	0.0	0.0	24.283437146450098	0.0
16	0.0	0.0	0.0	25.27432318193862	0.0
17	0.0	0.0	0.0	26.23997442874747	0.0
18	0.0	0.0	0.0	27.236748581594586	0.0
19	0.0	0.0	0.0	28.702048644260977	0.0
20	0.0	0.0	0.0	29.48348593370821	0.0
21	0.0	0.0	0.0	30.222865242022653	0.0
22	0.0	0.0	0.0	31.04720167222533	0.0
23	0.0	0.0	0.0	31.77101952752064	0.0
24	0.0	0.0	0.0	32.35226082677579	0.0
25	0.0	0.0	0.0	32.857797759991925	0.0
26	0.0	0.0	0.0	33.309500477358085	0.0
27	0.0	0.0	0.0	33.821346107744134	0.0
28	0.0	0.0	0.0	34.25748737209116	0.0
29	0.0	0.0	0.0	34.74662169266551	0.0
30	0.0	0.0	0.0	35.252999785504294	0.0
31	0.0	0.0	0.0	35.75264860136184	0.0
32	0.0	0.0	0.0	36.239259443068214	0.0
33	0.0	0.0	0.0	36.69853259703828	0.0
34	0.0	0.0	0.0	37.153599952895064	0.0
35	0.0	0.0	0.0	37.67301601988501	0.0
36	0.0	0.0	0.0	38.14869178649686	0.0
37	0.0	0.0	0.0	38.61427363763685	0.0
38	0.0	0.0	0.0	39.04789142311591	0.0
39	0.0	0.0	0.0	39.52987588689768	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGG	20	7.0219894E-4	45.000004	1
AAGGTTG	30	2.1582164E-6	45.000004	9
ATAGAGT	20	7.0219894E-4	45.000004	17
CATTCTA	30	2.1582164E-6	45.000004	45
TACGGGA	40	6.7757355E-9	45.000004	4
CCGTCAC	20	7.0219894E-4	45.000004	11
CTACGAA	40	6.7757355E-9	45.000004	11
TACTGAA	40	6.7757355E-9	45.000004	3
TTTGGGC	20	7.0219894E-4	45.000004	4
CTTTGCG	20	7.0219894E-4	45.000004	1
CGAATAT	40	6.7757355E-9	45.000004	14
CCGACTT	20	7.0219894E-4	45.000004	44
GTTCAAT	20	7.0219894E-4	45.000004	12
CTCGAGT	30	2.1582164E-6	45.000004	13
GCTACGA	40	6.7757355E-9	45.000004	10
CGGTAGT	20	7.0219894E-4	45.000004	12
AGCTACG	40	6.7757355E-9	45.000004	9
TTCGCAG	20	7.0219894E-4	45.000004	1
TAAGCCG	20	7.0219894E-4	45.000004	45
CTCTACA	20	7.0219894E-4	45.000004	21
>>END_MODULE