FastQCFastQC Report
Sat 18 Jun 2016
SRR3551907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319328
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC72012.2550481010121257No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC66932.0959640244513476No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG61731.9331220563182685No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34721.0872832949193305No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC27040.8467782342920132TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACT19010.5953126565788156No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT15020.47036276179977954No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT13150.4118022847980759No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC11830.37046547750275577No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTA11440.35825232989277483No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC10520.32944182783846077TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT6390.20010772622507264TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATG4110.1287077863513378No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC3560.11148411664495442TruSeq Adapter, Index 23 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3460.10835254033470289No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGTTCTAC3230.10114991482112436No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTT207.0256257E-445.00000442
GCGATAA207.0256257E-445.0000049
TCATCCG207.0256257E-445.0000041
GCTACGA207.0256257E-445.00000410
ATTGACG207.0256257E-445.0000041
TCGGTCG207.0256257E-445.00000415
GGCCATA253.8844577E-545.01
CTCGAAT650.044.99999643
CGTTTTT17150.043.688051
TCGAATG650.041.5384644
ATAAGGG1150.041.086963
CGAATGC556.002665E-1140.90909245
ACGGCTG9050.040.7734838
GTGTAAT501.0768417E-940.58
TATAGCG501.0768417E-940.51
TACGGCT9150.040.327877
GCATATC451.9208528E-840.00000428
AGGGTCC850.039.7058836
TGATACC8000.039.656254
ACCGGGA403.447767E-739.3750044