##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319328
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02851613388115	33.0	31.0	34.0	30.0	34.0
2	32.1335210191402	33.0	31.0	34.0	30.0	34.0
3	32.13214938871631	33.0	31.0	34.0	30.0	34.0
4	35.74818368574005	37.0	35.0	37.0	35.0	37.0
5	35.78871880949995	37.0	35.0	37.0	35.0	37.0
6	35.69036226074757	37.0	35.0	37.0	35.0	37.0
7	35.82593446237098	37.0	35.0	37.0	35.0	37.0
8	35.18035687443631	37.0	35.0	37.0	32.0	37.0
9	36.92522422086381	39.0	37.0	39.0	32.0	39.0
10	36.75215452450146	39.0	35.0	39.0	32.0	39.0
11	37.100648862611486	39.0	37.0	39.0	34.0	39.0
12	37.115370402845976	39.0	37.0	39.0	34.0	39.0
13	37.209596402445136	39.0	37.0	39.0	34.0	39.0
14	38.289686466579816	40.0	38.0	41.0	34.0	41.0
15	38.25875901893977	40.0	38.0	41.0	33.0	41.0
16	38.438887288305445	40.0	38.0	41.0	34.0	41.0
17	38.319737072852995	40.0	38.0	41.0	34.0	41.0
18	38.1866450796673	40.0	37.0	41.0	34.0	41.0
19	38.08695134783044	40.0	37.0	41.0	34.0	41.0
20	38.027100661388914	40.0	36.0	41.0	34.0	41.0
21	38.0446907255236	40.0	36.0	41.0	34.0	41.0
22	38.14498258843572	40.0	37.0	41.0	34.0	41.0
23	38.13819333099509	40.0	36.0	41.0	34.0	41.0
24	37.96201084778034	40.0	36.0	41.0	34.0	41.0
25	37.74824944884257	39.0	36.0	41.0	33.0	41.0
26	37.85095575708989	40.0	36.0	41.0	34.0	41.0
27	37.871974897284296	40.0	36.0	41.0	34.0	41.0
28	37.65614978955807	40.0	36.0	41.0	33.0	41.0
29	37.76036551758693	40.0	36.0	41.0	33.0	41.0
30	37.42979005912416	40.0	35.0	41.0	33.0	41.0
31	37.25909096602866	39.0	35.0	41.0	33.0	41.0
32	37.198773674716904	40.0	35.0	41.0	33.0	41.0
33	37.070394703878144	40.0	35.0	41.0	32.0	41.0
34	36.93251139893777	40.0	35.0	41.0	31.0	41.0
35	36.876712972241705	40.0	35.0	41.0	31.0	41.0
36	36.63570999098106	40.0	35.0	41.0	30.0	41.0
37	36.636185990580216	40.0	35.0	41.0	31.0	41.0
38	36.41875751578314	39.0	35.0	41.0	30.0	41.0
39	36.41945585730033	39.0	35.0	41.0	30.0	41.0
40	36.37571086782243	39.0	35.0	41.0	30.0	41.0
41	36.1704235143802	39.0	35.0	41.0	29.0	41.0
42	36.31734454855196	39.0	35.0	41.0	30.0	41.0
43	36.263246567792365	39.0	35.0	41.0	30.0	41.0
44	36.365708613087484	39.0	35.0	41.0	30.0	41.0
45	36.35046409960918	39.0	35.0	41.0	30.0	41.0
46	36.27076235093697	39.0	35.0	41.0	30.0	41.0
47	36.12445197414571	39.0	35.0	41.0	30.0	41.0
48	36.14667050806694	39.0	35.0	41.0	30.0	41.0
49	36.135146307245215	39.0	35.0	41.0	30.0	41.0
50	36.016359354644756	39.0	35.0	41.0	30.0	41.0
51	35.2576065988576	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	4.0
12	6.0
13	7.0
14	5.0
15	23.0
16	24.0
17	37.0
18	75.0
19	175.0
20	387.0
21	695.0
22	1039.0
23	1401.0
24	1995.0
25	2831.0
26	3738.0
27	4251.0
28	4238.0
29	4167.0
30	4607.0
31	5372.0
32	6785.0
33	9493.0
34	17243.0
35	22708.0
36	22420.0
37	34589.0
38	67047.0
39	103922.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.006613889167255	16.162065337208137	18.139029461869928	19.692291311754683
2	30.646232087383506	32.150954504459364	19.577988776430505	17.624824631726625
3	28.09650265557671	30.544455857300328	25.993962320873838	15.365079166249124
4	26.768714300030062	20.393138089988977	35.388378093997396	17.449769515983565
5	30.64779787553863	23.51845124762	24.984342118448744	20.849408758392624
6	22.84015181881952	36.50791662491232	26.066928048902692	14.585003507365467
7	66.62491231586331	5.941226575809199	21.418729331596353	6.015131776731136
8	64.53239302535324	12.785912917125962	15.100774125663893	7.5809199318569
9	59.41946838360557	7.345425393325984	16.163631125363263	17.07147509770518
10	39.27278534923339	19.532894077562883	24.14132177572903	17.052998797474697
11	30.854168754384208	21.72499749473895	26.97978254334102	20.441051207535825
12	26.306493636636937	18.32567140996092	31.648962821926048	23.718872131476097
13	24.328276881451046	20.72289307545846	35.915422387012725	19.033407656077763
14	19.65377292313859	28.31226826335304	29.23420432909109	22.799754484417274
15	16.962809399739452	22.889004409259446	39.21673013327989	20.931456057721213
16	18.620979056017635	22.741820322677622	31.522447138991883	27.11475348231286
17	18.655113237799377	25.62036526706083	32.399601663493335	23.324919831646458
18	19.158044393225772	24.297274275979557	31.958049403747868	24.5866319270468
19	20.281340815712998	25.48445485519591	29.53671460066139	24.6974897284297
20	24.139755987573906	24.828389618198216	32.81390920933961	18.217945184888265
21	22.500062631526205	28.519578615091696	29.392035775127766	19.588322978254332
22	19.48654674817116	22.977941176470587	31.89291261649464	25.642599458863614
23	21.38584778033871	26.828527407555868	30.547587433610584	21.23803737849484
24	22.691401944082575	23.024288505862312	29.087959715402345	25.19634983465277
25	18.665760597254234	30.825045094698865	27.464863713798977	23.04433059424792
26	19.084765507565887	24.276605872331896	31.393113037378495	25.24551558272372
27	25.042902595450446	25.465352239703375	27.60797675117747	21.883768413668705
28	17.80019290510071	25.522033770918927	33.354106122858	23.323667201122355
29	21.88909209339613	21.414345124762	30.432658583024352	26.263904198817517
30	22.402357450646356	25.543328489828642	29.674503958312453	22.379810101212545
31	21.580005511574306	22.925330694458363	29.473769916825333	26.020893877141997
32	23.530351237598957	26.530088185188898	28.58659434813108	21.35296622908107
33	21.511737148010823	22.41770217456659	26.52288555967532	29.547675117747268
34	20.80713247820423	22.96541487122958	30.91554765006514	25.31190500050105
35	22.148699769515982	24.103742860006015	26.40889618198216	27.338661188495838
36	19.81003858102014	24.09121655476501	27.9120528109029	28.18669205331196
37	22.024063032367973	23.233790961018137	33.345024551558275	21.39712145505562
38	19.050944483415172	24.093721815813208	28.56216805291111	28.29316564786051
39	21.295658382603467	21.78606323278886	32.82800130273574	24.090277081871932
40	20.09031466078765	23.487761799779534	30.90114239903798	25.520781140394828
41	18.82108678224271	24.680579216354346	29.37919631225574	27.11913768914721
42	20.381551257641046	24.92891321775729	30.79122407054815	23.898311454053513
43	22.538267862511276	22.248597053813008	27.369976951598357	27.843158132077363
44	21.80140795670909	22.133041887964726	30.205306142900092	25.860244012426094
45	20.530927447640043	21.05515332197615	28.978980859805592	29.434938370578212
46	24.300092694658783	24.06397184086582	28.35704980458964	23.27888565988576
47	17.999048000801686	23.062180579216353	35.47261749674316	23.466153923238803
48	20.50149063032368	23.25884357150015	29.247043791963122	26.992622006213047
49	19.446775729030964	21.077387513778937	35.15914670808698	24.31669004910312
50	20.754521996192	20.302009219360656	31.615454955406353	27.328013829040987
51	19.550117747269265	21.0084928349534	28.430015532618498	31.011373885158832
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	251.5
2	385.0
3	3083.5
4	5782.0
5	3857.0
6	1932.0
7	1846.0
8	1760.0
9	1719.0
10	1678.0
11	1630.5
12	1583.0
13	1500.5
14	1418.0
15	1333.0
16	1248.0
17	1171.5
18	1095.0
19	1023.0
20	951.0
21	964.5
22	978.0
23	1056.5
24	1135.0
25	1345.5
26	1888.5
27	2221.0
28	2506.5
29	2792.0
30	3228.0
31	3664.0
32	4115.5
33	4567.0
34	5057.0
35	5547.0
36	5921.0
37	6295.0
38	7043.0
39	7791.0
40	8941.0
41	10091.0
42	11458.5
43	12826.0
44	15220.5
45	17615.0
46	33162.5
47	48710.0
48	40203.5
49	31697.0
50	31445.0
51	31193.0
52	26773.5
53	22354.0
54	19806.0
55	17258.0
56	15971.5
57	14685.0
58	13749.5
59	12814.0
60	12134.5
61	11455.0
62	10581.0
63	9707.0
64	8498.0
65	7289.0
66	6311.0
67	5333.0
68	4448.0
69	3563.0
70	3331.0
71	3099.0
72	2560.5
73	2022.0
74	1672.5
75	1046.5
76	770.0
77	692.0
78	614.0
79	438.5
80	263.0
81	187.0
82	111.0
83	67.5
84	24.0
85	17.0
86	10.0
87	6.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	319328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.79994253531645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03137988893454	22.970252360259682
2	11.038081143873635	7.2409685443843035
3	4.086962917530948	4.021564465169529
4	2.235884821117671	2.9334757459298357
5	1.5606327812347192	2.559433277161493
6	1.1935644451455905	2.348930712778333
7	1.060932902199574	2.4358976758180764
8	0.9422740853874298	2.4725228682580447
9	0.92332860838513	2.725661276662137
>10	6.819269978534588	35.24815545415629
>50	0.059826904253439527	1.346477901973248
>100	0.03589614255206371	2.2566668748154997
>500	9.971150708906588E-4	0.20898697579813102
>1k	0.00797692056712527	4.668029899165453
>5k	0.0029913452126719762	6.562975967669947
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	7201	2.2550481010121257	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	6693	2.0959640244513476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	6173	1.9331220563182685	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3472	1.0872832949193305	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	2704	0.8467782342920132	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACT	1901	0.5953126565788156	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	1502	0.47036276179977954	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	1315	0.4118022847980759	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC	1183	0.37046547750275577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTA	1144	0.35825232989277483	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1052	0.32944182783846077	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	639	0.20010772622507264	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATG	411	0.1287077863513378	No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	356	0.11148411664495442	TruSeq Adapter, Index 23 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	346	0.10835254033470289	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGTTCTAC	323	0.10114991482112436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20417877542839963	0.0
2	0.0	0.0	0.0	1.6093170658382603	0.0
3	0.0	0.0	0.0	1.9882377993786953	0.0
4	0.0	0.0	0.0	2.8569370678424693	0.0
5	0.0	0.0	0.0	6.265657881551258	0.0
6	0.0	0.0	0.0	7.256801783745867	0.0
7	0.0	0.0	0.0	8.580519090089187	0.0
8	0.0	0.0	0.0	10.193280889868724	0.0
9	0.0	0.0	0.0	10.68399889768514	0.0
10	0.0	0.0	0.0	13.583212245716004	0.0
11	0.0	0.0	0.0	15.087308347529813	0.0
12	0.0	0.0	0.0	18.589663292915123	0.0
13	0.0	0.0	0.0	19.22756538731336	0.0
14	0.0	0.0	0.0	19.542915121755687	0.0
15	0.0	0.0	0.0	20.29104870227478	0.0
16	0.0	0.0	0.0	21.147221665497543	0.0
17	0.0	0.0	0.0	22.022497244212847	0.0
18	0.0	0.0	0.0	22.848920232488226	0.0
19	0.0	0.0	0.0	24.027958713297927	0.0
20	3.131576310251528E-4	0.0	0.0	24.728179176270167	0.0
21	3.131576310251528E-4	0.0	0.0	25.42401543240806	0.0
22	3.131576310251528E-4	0.0	0.0	26.15774376189999	0.0
23	3.131576310251528E-4	0.0	0.0	26.83416424491432	0.0
24	3.131576310251528E-4	0.0	0.0	27.393463773925244	0.0
25	3.131576310251528E-4	0.0	0.0	27.911426495640846	0.0
26	3.131576310251528E-4	0.0	0.0	28.35892875037579	0.0
27	3.131576310251528E-4	0.0	0.0	28.878770417877544	0.0
28	3.131576310251528E-4	0.0	0.0	29.312806894478406	0.0
29	3.131576310251528E-4	0.0	0.0	29.784422286802286	0.0
30	3.131576310251528E-4	0.0	0.0	30.271069245415372	0.0
31	6.263152620503056E-4	0.0	0.0	30.784021445034572	0.0
32	6.263152620503056E-4	0.0	0.0	31.27129471890971	0.0
33	6.263152620503056E-4	0.0	0.0	31.739465377292312	0.0
34	6.263152620503056E-4	0.0	0.0	32.189159735444434	0.0
35	6.263152620503056E-4	0.0	0.0	32.64417777332398	0.0
36	6.263152620503056E-4	0.0	0.0	33.15149313558473	0.0
37	6.263152620503056E-4	0.0	0.0	33.61684537528811	0.0
38	6.263152620503056E-4	0.0	0.0	34.08877392524301	0.0
39	6.263152620503056E-4	0.0	0.0	34.57479456859405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGTT	20	7.0256257E-4	45.000004	42
GCGATAA	20	7.0256257E-4	45.000004	9
TCATCCG	20	7.0256257E-4	45.000004	1
GCTACGA	20	7.0256257E-4	45.000004	10
ATTGACG	20	7.0256257E-4	45.000004	1
TCGGTCG	20	7.0256257E-4	45.000004	15
GGCCATA	25	3.8844577E-5	45.0	1
CTCGAAT	65	0.0	44.999996	43
CGTTTTT	1715	0.0	43.68805	1
TCGAATG	65	0.0	41.53846	44
ATAAGGG	115	0.0	41.08696	3
CGAATGC	55	6.002665E-11	40.909092	45
ACGGCTG	905	0.0	40.773483	8
GTGTAAT	50	1.0768417E-9	40.5	8
TATAGCG	50	1.0768417E-9	40.5	1
TACGGCT	915	0.0	40.32787	7
GCATATC	45	1.9208528E-8	40.000004	28
AGGGTCC	85	0.0	39.705883	6
TGATACC	800	0.0	39.65625	4
ACCGGGA	40	3.447767E-7	39.375004	4
>>END_MODULE