FastQCFastQC Report
Sat 18 Jun 2016
SRR3551906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338301
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC82722.44515978374288No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC82262.4315624251775785No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG66611.9689566392059143No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37461.1072979388177984No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC27750.8202754351893727No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT18360.5427119636063742No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT16420.485366581830973No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT15380.4546247276833353No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC13180.3895938823710246No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA12660.3742229552972058No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC12360.36535511275461796No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT6580.19450134643409273No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG4710.13922512791862868No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT4400.13006169062462128No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3780.11173481603660645No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT3490.10316256824543822Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACTTTGCG3440.10168459448834026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAC302.1609449E-645.00000418
ACAACAT207.026223E-445.036
CCCTCGT351.2085366E-745.014
CAGCACG207.026223E-445.01
ACGATAG207.026223E-445.01
ATATCAC207.026223E-445.030
CGAATGC900.045.045
GCGATTA351.2085366E-745.09
GTATATG207.026223E-445.01
GTAGTAG406.7902874E-945.01
CATACGG253.8849517E-544.9999962
TTACGGG650.044.9999963
TTTGCGG253.8849517E-544.9999962
CGTTTTT19250.044.181821
CGTCGAA1050.042.85714742
TACGGCT9900.042.0454527
CTTGCGG650.041.538462
GATGAAT5050.041.43564620
CCGATGA4800.041.25000418
ACGGCTG10200.041.258