##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338301
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03062361624707	33.0	31.0	34.0	30.0	34.0
2	32.141498251557046	33.0	31.0	34.0	30.0	34.0
3	32.14651745043615	33.0	31.0	34.0	30.0	34.0
4	35.759708070623496	37.0	35.0	37.0	35.0	37.0
5	35.79162343593427	37.0	35.0	37.0	35.0	37.0
6	35.69611972769812	37.0	35.0	37.0	35.0	37.0
7	35.83108533524879	37.0	35.0	37.0	35.0	37.0
8	35.166378461784035	37.0	35.0	37.0	32.0	37.0
9	36.9366156174531	39.0	37.0	39.0	32.0	39.0
10	36.77766840771975	39.0	35.0	39.0	33.0	39.0
11	37.084546010801034	39.0	37.0	39.0	34.0	39.0
12	37.07138613246783	39.0	37.0	39.0	33.0	39.0
13	37.16196818809285	39.0	37.0	39.0	34.0	39.0
14	38.25549436744201	40.0	38.0	41.0	34.0	41.0
15	38.21507769708041	40.0	37.0	41.0	33.0	41.0
16	38.392975486327266	40.0	38.0	41.0	34.0	41.0
17	38.28265952509747	40.0	37.0	41.0	34.0	41.0
18	38.133295497205154	40.0	37.0	41.0	34.0	41.0
19	38.04509002338155	40.0	37.0	41.0	34.0	41.0
20	37.959896660074904	40.0	36.0	41.0	34.0	41.0
21	37.965799687260755	40.0	36.0	41.0	34.0	41.0
22	38.0517024779708	40.0	36.0	41.0	34.0	41.0
23	38.05605067676419	40.0	36.0	41.0	34.0	41.0
24	37.90847499711795	40.0	36.0	41.0	34.0	41.0
25	37.72348588978454	39.0	35.0	41.0	33.0	41.0
26	37.79258116292887	40.0	36.0	41.0	33.0	41.0
27	37.830609427699	40.0	36.0	41.0	34.0	41.0
28	37.65468916733914	40.0	35.0	41.0	33.0	41.0
29	37.69976736693063	40.0	36.0	41.0	33.0	41.0
30	37.402629610908626	40.0	35.0	41.0	33.0	41.0
31	37.30921280161749	39.0	35.0	41.0	33.0	41.0
32	37.223082402948855	40.0	35.0	41.0	33.0	41.0
33	37.108152207649404	40.0	35.0	41.0	32.0	41.0
34	36.887195130963256	40.0	35.0	41.0	31.0	41.0
35	36.81017200658585	40.0	35.0	41.0	31.0	41.0
36	36.649752734990436	40.0	35.0	41.0	31.0	41.0
37	36.66171545458039	40.0	35.0	41.0	31.0	41.0
38	36.43324140336564	39.0	35.0	41.0	30.0	41.0
39	36.35919491813503	39.0	35.0	41.0	30.0	41.0
40	36.23798333436792	39.0	35.0	41.0	30.0	41.0
41	36.055654579797284	39.0	35.0	41.0	29.0	41.0
42	36.23323608266011	39.0	35.0	41.0	30.0	41.0
43	36.14112580217026	39.0	35.0	41.0	30.0	41.0
44	36.23091861980899	39.0	35.0	41.0	30.0	41.0
45	36.24763154705425	39.0	35.0	41.0	30.0	41.0
46	36.16686914907139	39.0	35.0	41.0	30.0	41.0
47	35.99724801286428	39.0	35.0	41.0	29.0	41.0
48	35.97746385615177	39.0	35.0	41.0	29.0	41.0
49	35.983236821647	39.0	35.0	41.0	30.0	41.0
50	35.868619956784045	39.0	35.0	41.0	29.0	41.0
51	35.00405851593699	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	4.0
12	4.0
13	4.0
14	14.0
15	14.0
16	24.0
17	59.0
18	91.0
19	186.0
20	419.0
21	744.0
22	1195.0
23	1606.0
24	2051.0
25	2811.0
26	4024.0
27	4720.0
28	4512.0
29	4467.0
30	4837.0
31	5752.0
32	7300.0
33	10319.0
34	18951.0
35	24857.0
36	24641.0
37	37653.0
38	69442.0
39	107565.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.279496661257284	15.992858430805704	17.74100579070118	19.986639117235836
2	30.39482590947115	32.643710778271426	20.082707411447203	16.878755900810223
3	27.475236549699822	30.92187135125229	26.28132934871608	15.321562750331804
4	26.234625377991787	20.589356815380384	35.78056228033615	17.395455526291677
5	31.257962583616365	23.067327616530843	25.048403640544958	20.626306159307838
6	22.284001525268916	36.51659321137094	26.534358455931255	14.665046807428888
7	66.9356578904585	5.335189668372249	21.93460852909096	5.794543912078296
8	64.05479144312314	13.240575700337864	15.381568484869982	7.323064371669016
9	59.5856943964103	7.191229112535878	15.893834189080138	17.329242301973686
10	40.573926769356284	19.780609575496378	23.771434314412314	15.874029340735026
11	31.574544562386752	21.333664399454925	26.428239940171622	20.663551097986705
12	26.22043682992365	18.10990804047283	31.28574849024981	24.38390663935371
13	24.57279168551083	20.3466735244649	35.96146626820494	19.119068521819326
14	20.333371760651016	27.410796893890353	28.869556992146045	23.386274353312583
15	17.273966083458223	22.908593235018518	38.571567923239954	21.245872758283305
16	19.041327102195975	23.805427710825565	30.144752749770177	27.008492437208286
17	18.938755723453372	25.202999695537404	32.09006180886252	23.768182772146698
18	19.090987020434465	24.044563864724015	31.769637098323685	25.094812016517835
19	21.308243250832838	24.726205361497602	28.38832873683495	25.577222650834614
20	23.64196381329053	24.457214137705773	33.53522454855292	18.365597500450782
21	22.55033239629797	28.462523019441267	29.862459762164463	19.1246848220963
22	20.14241755123396	21.65083756772815	32.0655274444947	26.141217436543197
23	21.241438837012012	27.13382461181019	30.26594659785221	21.358789953325587
24	23.422041318234356	22.957366369002752	28.706093094611013	24.914499218151885
25	18.638431456011066	30.64194312165793	27.63604009447208	23.08358532785892
26	19.59674964011339	24.481452907322176	30.614452809775912	25.30734464278852
27	23.94701759675555	25.440953470430177	27.344583669572366	23.267445263241907
28	18.093945923896175	25.262709835324166	33.26091261923553	23.38243162154413
29	20.823467858504703	24.689551612321573	29.93606285526794	24.55091767390578
30	19.599705587627586	25.55416626022388	30.935468709817588	23.910659442330942
31	23.006139502986986	23.052252284208443	29.021492694375716	24.920115518428855
32	22.344598449309935	26.19590246555582	29.388916970390273	22.07058211474397
33	22.112852164196973	21.71527722353762	29.176384344119587	26.995486268145825
34	24.64609918386289	24.259461249006062	28.268908457261432	22.825531109869612
35	21.129408426223982	21.535555614674507	26.512188849574787	30.822847109526723
36	23.966231255597826	26.148607305328685	27.517506599152824	22.36765483992066
37	20.98131545576277	21.975105010035442	32.42053674094963	24.62304279325216
38	20.87697050851165	21.78799353238684	31.412854233360232	25.92218172574128
39	25.90562841966178	21.881401473835428	31.215988128914784	20.996981977588007
40	21.380663964930637	21.12704366821263	32.69987378104114	24.79241858581559
41	24.568948953742378	24.05845681804074	25.94198657408639	25.430607654130494
42	21.265382011876994	21.314746335364067	30.951726421145665	26.468145231613267
43	26.60618798052622	21.97569619953828	27.35492948587205	24.063186334063452
44	22.708179993556037	21.398104055264394	30.136771691481844	25.756944259697722
45	20.953233954377907	19.64049766332349	29.48114253283319	29.925125849465417
46	26.279851374958984	23.411695501934666	27.45779645936607	22.850656663740278
47	18.944076428978924	22.053733213913056	36.344261471293315	22.657928885814705
48	21.75902524674772	22.77114167560841	28.681558730243182	26.788274347400687
49	20.6514317131785	19.527580468281204	35.484671934165135	24.336315884375157
50	22.967120995799597	19.680698549516553	31.5978965477489	25.75428390693495
51	20.69458854688576	20.189712711461095	28.076771868838694	31.03892687281445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	234.5
2	387.0
3	3406.0
4	6425.0
5	4253.0
6	2081.0
7	2054.5
8	2028.0
9	1911.0
10	1794.0
11	1737.5
12	1681.0
13	1578.5
14	1476.0
15	1387.5
16	1299.0
17	1212.5
18	1126.0
19	1080.0
20	1034.0
21	1032.5
22	1031.0
23	1032.5
24	1034.0
25	1313.0
26	1839.5
27	2087.0
28	2387.5
29	2688.0
30	3099.0
31	3510.0
32	4024.5
33	4539.0
34	4957.0
35	5375.0
36	5590.5
37	5806.0
38	6556.0
39	7306.0
40	8467.0
41	9628.0
42	11322.0
43	13016.0
44	15329.0
45	17642.0
46	35458.5
47	53275.0
48	42955.5
49	32636.0
50	32565.0
51	32494.0
52	27882.0
53	23270.0
54	21201.0
55	19132.0
56	17327.0
57	15522.0
58	14621.0
59	13720.0
60	13275.5
61	12831.0
62	11892.0
63	10953.0
64	10029.0
65	9105.0
66	7646.0
67	6187.0
68	5389.5
69	4592.0
70	3988.5
71	3385.0
72	3009.0
73	2633.0
74	2154.0
75	1294.5
76	914.0
77	795.5
78	677.0
79	524.0
80	371.0
81	252.5
82	134.0
83	116.5
84	99.0
85	57.0
86	15.0
87	8.5
88	2.0
89	5.5
90	9.0
91	6.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	338301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.32404511045571
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20638978376192	25.859458150952104
2	9.661853863448336	6.825915234461596
3	3.664085948042902	3.8829101195176303
4	2.0403690563328643	2.882963543515202
5	1.449511481920801	2.560130448774694
6	1.1771605425962082	2.4949243265350183
7	1.0117962167493395	2.5018514642129466
8	0.9496974702593811	2.683772502458244
9	0.830562997155394	2.6404960300733187
>10	5.903703670749691	32.20602118145774
>50	0.06543823664547994	1.5908159002834175
>100	0.02936331131527946	2.261459786710648
>500	8.389517518651276E-4	0.19499941549673783
>1k	0.0067116140149210204	4.551072984473256
>5k	0.0025168552555953827	6.863208911077434
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTC	8272	2.44515978374288	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGC	8226	2.4315624251775785	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCG	6661	1.9689566392059143	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3746	1.1072979388177984	No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	2775	0.8202754351893727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTTTGCGT	1836	0.5427119636063742	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCT	1642	0.485366581830973	No Hit
GAACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCT	1538	0.4546247276833353	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTC	1318	0.3895938823710246	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTTTGCGTCGTA	1266	0.3742229552972058	No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC	1236	0.36535511275461796	No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCT	658	0.19450134643409273	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATG	471	0.13922512791862868	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTT	440	0.13006169062462128	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	378	0.11173481603660645	No Hit
TGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGCTT	349	0.10316256824543822	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACTTTGCG	344	0.10168459448834026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19834407820254743	0.0
2	0.0	0.0	0.0	1.592960115400191	0.0
3	0.0	0.0	0.0	1.961862365171844	0.0
4	0.0	0.0	0.0	2.857514461973213	0.0
5	0.0	0.0	0.0	6.54210303841845	0.0
6	0.0	0.0	0.0	7.510767038820458	0.0
7	0.0	0.0	0.0	8.859270294796646	0.0
8	0.0	0.0	0.0	10.42710485632617	0.0
9	0.0	0.0	0.0	10.88705028953506	0.0
10	0.0	0.0	0.0	13.840041856216802	0.0
11	0.0	0.0	0.0	15.327179050608777	0.0
12	0.0	0.0	0.0	19.070886577337934	0.0
13	0.0	0.0	0.0	19.751641289857258	0.0
14	0.0	0.0	0.0	20.066154105367705	0.0
15	0.0	0.0	0.0	20.79686433087694	0.0
16	0.0	0.0	0.0	21.596152538715522	0.0
17	0.0	0.0	0.0	22.404012994345273	0.0
18	0.0	0.0	0.0	23.231087108817295	0.0
19	0.0	0.0	0.0	24.342227779403547	0.0
20	0.0	0.0	0.0	24.979234468712775	0.0
21	0.0	0.0	0.0	25.648756580678153	0.0
22	0.0	0.0	0.0	26.324781777174763	0.0
23	0.0	0.0	0.0	26.983662478089038	0.0
24	0.0	0.0	0.0	27.54085858451497	0.0
25	0.0	0.0	0.0	28.05933177850494	0.0
26	2.955947514195938E-4	0.0	0.0	28.507453421657043	0.0
27	2.955947514195938E-4	0.0	0.0	29.001983440782027	0.0
28	2.955947514195938E-4	0.0	0.0	29.4571993579682	0.0
29	2.955947514195938E-4	0.0	0.0	29.92305668620548	0.0
30	2.955947514195938E-4	0.0	0.0	30.42527216886737	0.0
31	2.955947514195938E-4	0.0	0.0	30.922166946003706	0.0
32	2.955947514195938E-4	0.0	0.0	31.402804011811966	0.0
33	2.955947514195938E-4	0.0	0.0	31.866592176789307	0.0
34	2.955947514195938E-4	0.0	0.0	32.2934309978392	0.0
35	2.955947514195938E-4	0.0	0.0	32.764904626353456	0.0
36	5.911895028391876E-4	0.0	0.0	33.236969444370544	0.0
37	5.911895028391876E-4	0.0	0.0	33.70134879885073	0.0
38	5.911895028391876E-4	0.0	0.0	34.13764665194605	0.0
39	5.911895028391876E-4	0.0	0.0	34.581334373826856	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGTAC	30	2.1609449E-6	45.000004	18
ACAACAT	20	7.026223E-4	45.0	36
CCCTCGT	35	1.2085366E-7	45.0	14
CAGCACG	20	7.026223E-4	45.0	1
ACGATAG	20	7.026223E-4	45.0	1
ATATCAC	20	7.026223E-4	45.0	30
CGAATGC	90	0.0	45.0	45
GCGATTA	35	1.2085366E-7	45.0	9
GTATATG	20	7.026223E-4	45.0	1
GTAGTAG	40	6.7902874E-9	45.0	1
CATACGG	25	3.8849517E-5	44.999996	2
TTACGGG	65	0.0	44.999996	3
TTTGCGG	25	3.8849517E-5	44.999996	2
CGTTTTT	1925	0.0	44.18182	1
CGTCGAA	105	0.0	42.857147	42
TACGGCT	990	0.0	42.045452	7
CTTGCGG	65	0.0	41.53846	2
GATGAAT	505	0.0	41.435646	20
CCGATGA	480	0.0	41.250004	18
ACGGCTG	1020	0.0	41.25	8
>>END_MODULE