SRR3551905_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	224827
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGC	7949	3.5356073781173967	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTC	6908	3.0725846984570357	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCG	6143	2.7323230750755023	No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	2786	1.2391750101188914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	1.2062608138702202	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACGAGTCTT	1772	0.7881615642249373	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCT	1579	0.7023177821169166	No Hit
GAACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCT	1423	0.6329310981332313	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTA	1263	0.5617652684063746	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTC	1219	0.5421946652314891	No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	926	0.41187223954418284	No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCT	576	0.256196987016684	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATG	458	0.20371218759312718	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTT	344	0.1530065339127418	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACGAGTCT	316	0.14055251371054187	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTAT	297	0.13210157143047765	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACGAGTCTTCGT	291	0.12943285281572053	No Hit
TCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	285	0.1267641342009634	No Hit
TGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGCTT	251	0.11164139538400637	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCC	237	0.1054143852829064	No Hit
ACTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC	228	0.10141130736077072	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATAAG	20	7.021168E-4	45.000004	12
CCTTAGG	30	2.1576889E-6	45.000004	23
CAGTTAT	20	7.021168E-4	45.000004	36
TATATGG	30	2.1576889E-6	45.000004	2
TAACGAG	20	7.021168E-4	45.000004	1
ACGGGTA	20	7.021168E-4	45.000004	5
ACGGGAC	20	7.021168E-4	45.000004	5
TCAATGG	20	7.021168E-4	45.000004	2
CCATCGA	30	2.1576889E-6	45.000004	41
ATGAGTC	20	7.021168E-4	45.000004	38
CACTAGG	20	7.021168E-4	45.000004	2
GTGTAAG	20	7.021168E-4	45.000004	1
TTGCGCG	20	7.021168E-4	45.000004	1
ACTAATC	20	7.021168E-4	45.000004	10
GCGATGT	20	7.021168E-4	45.000004	9
GTATCCA	20	7.021168E-4	45.000004	24
TTAGTCA	20	7.021168E-4	45.000004	32
TTTGCGT	20	7.021168E-4	45.000004	18
ATTGCGG	40	6.7720975E-9	45.000004	2
ACGAGAG	20	7.021168E-4	45.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content